Isolation and Characterization of Thermophilic Bacteria from Microbial Mats Present Carpet Comanjilla Geothermal Hot Spring, México

Cellulase enzymes can be isolated from thermophile bacteria obtained from the hot spring Ie Seuum, Aceh Besar. This research aimed to recover and characterize the isolates morphologically and biochemically followed by determination of the thermophile bacterial isolates potential as cellulolytic enzyme producers,. The sampling method in this research was conducted by a purposive sampling at temperature of 70 oC, 60 oC and 50 oC. Isolation of thermophilic bacteria was carried out on nutrient agar (NA) media. There were four isolates of thermophilic bacteria isolated recovered at 70 oC, five isolates at 60 oC, and seven isolates at 50 oC. Of the 18 isolates obtained, 15 of them were able to produce cellulase enzymes. Cellulase enzyme production can be determined by the presence of clear zones around bacterial colonies on CMC media after addition of 1% congo red drops and wash with 1 M NaCl. The highest five Cellulolytic Index (CI) values were obtained from isolates ISB75; ISB64; ISB52; ISB54; ISB56 that were 1.23; 2.22; 1.39; 1.59; 1.10, respectively. Biochemical tests carried out on 5 isolates with the highest cellulolytic index values showed that the bacterial isolate were suspected to be from the genera of Bacillus sp.

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Short Communication: Isolation and characterization of thermophilic actinobacteria as proteolytic enzyme producer from Ie Seuum Hot Spring, Aceh Besar, Indonesia

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d201004 ◽

2019 ◽

Vol 20 (10) ◽

Cited By ~ 1

Author(s):

Lenni Fitri ◽

KARTINI AMELIA PUTRI ◽

SUHARTONO ◽

YULIA SARI ISMAIL

Keyword(s):

Proteolytic Enzyme ◽

Sampling Method ◽

Short Communication ◽

Hot Spring ◽

Thermophilic Bacteria ◽

Skim Milk ◽

Morphological Characterization ◽

Peptide Bonds ◽

Isolation And Characterization

Abstract. Fitri L, Putri KA, Suhartono, Ismail YS. 2019. Short Communication: Isolation and characterization of thermophilic actinobacteria as proteolytic enzyme producer from Ie Seuum Hot Spring, Aceh Besar, Indonesia. Biodiversitas 20: 2802-2808. Proteases are enzymes who catalyze the breakdown of peptide bonds in proteins. This enzyme could be produced from thermophilic bacteria that are able to grow at temperatures of 45-80ºC and are stable to heat. The samples were collected at Ie Seu'um hot spring, Aceh Besar. This study aimed to isolate, to characterize and to determine actinobacteria that were capable of producing protease enzymes. The sampling method in this study was conducted by purposive sampling at temperatures of 50, 60 and 70ºC. Isolation of thermophilic actinobacteria was carried out in Humic Acid Vitamin B (HV) Agar medium and morphological characterization was carried out in Yeast Malt Agar (YMA), Yeast Starch Agar (YSA), Oatmeal Agar (OA) media. Microscopic characterization and measurement of clear zone diameter formed were carried out in skim milk medium. The results showed that one actinobacteria isolate was obtained at a temperature of 50ºC and five isolates at a temperature of 60ºC, meanwhile, no actinobacteria could be obtained at a temperature of 70ºC. A total of 4 isolates obtained were able to produce protease enzymes. The highest Proteolytic Index (IP) value was obtained from IS01 which was 3.8.

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PRODUCTION AND CHARACTERIZATION OF CHITINASE ENZYMES FROM SULILI HOT SPRING IN SOUTH SULAWESI, Bacillus sp. HSA,3-1a

Indonesian Journal of Chemistry ◽

10.22146/ijc.21470 ◽

2010 ◽

Vol 10 (2) ◽

pp. 256-260 ◽

Cited By ~ 6

Author(s):

Hasnah Natsir ◽

Abd. Rauf Patong ◽

Maggy Thenawidjaja Suhartono ◽

Ahyar Ahmad

Keyword(s):

Hot Spring ◽

Thermophilic Bacteria ◽

Extracellular Enzyme ◽

Colloidal Chitin ◽

Bacillus Sp ◽

Optimum Temperature ◽

South Sulawesi ◽

Sds Page ◽

Physiological Analysis

Chitinase is an extracellular enzyme which is capable in hydrolyzing insoluble chitin to its oligomeric and monomeric components. The enzyme produced by thermophilic bacteria was screened and isolated from Sulili hot spring in Pinrang, South Sulawesi, Indonesia. The gram positive spore forming rod shape bacteria was identified as Bacillus sp. HSA,3-1a through morphological and physiological analysis. The production of chitinase enzyme was conducted at various concentration of colloidal chitin at a pH of 7.0 and a temperature of 55 °C. The bacteria optimally was produced the enzyme at a colloidal chitin concentration of 0.5% after 72 h of incubation. The optimum temperature, pH and substrate concentration of chitinase were 60 °C, 7.0 and 0.3%, respectively. The enzyme was stable at a pH of 7.0 and a temperature of 60 °C after 2 h of incubation. The chitinase activities was increased by addition of 1 mM Mg2+, Ca2+ and Mn2+ ions, whereas the activities were decreased by 1 mM Co2+, Fe2+ and Zn2 ions. The molecular weight of the crude enzyme was determined using SDS-PAGE analysis. Five protein fractions were obtained from SDS-PAGE, with MWs of 79, 71, 48, 43 and 22 kDa. Keywords: colloidal chitin, thermophilic bacteria, chitinase

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Isolation and characterization of a thermophilic sulfur- and iron-reducing thaumarchaeote from a terrestrial acidic hot spring

The ISME Journal ◽

10.1038/s41396-019-0447-3 ◽

2019 ◽

Vol 13 (10) ◽

pp. 2465-2474 ◽

Cited By ~ 9

Author(s):

Shingo Kato ◽

Takashi Itoh ◽

Masahiro Yuki ◽

Mai Nagamori ◽

Masafumi Ohnishi ◽

...

Keyword(s):

Hot Spring ◽

Isolation And Characterization

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Cultivation and Genomic, Nutritional, and Lipid Biomarker Characterization of Roseiflexus Strains Closely Related to Predominant In Situ Populations Inhabiting Yellowstone Hot Spring Microbial Mats

Journal of Bacteriology ◽

10.1128/jb.01610-09 ◽

2010 ◽

Vol 192 (12) ◽

pp. 3033-3042 ◽

Cited By ~ 46

Author(s):

Marcel T. J. van der Meer ◽

Christian G. Klatt ◽

Jason Wood ◽

Donald A. Bryant ◽

Mary M. Bateson ◽

...

Keyword(s):

National Park ◽

Microbial Mats ◽

Hot Spring ◽

Small Subunit ◽

Small Subunit Rrna ◽

Anoxygenic Phototrophs ◽

Lipid Biomarker ◽

Genes Encoding

ABSTRACT Roseiflexus sp. strains were cultivated from a microbial mat of an alkaline siliceous hot spring in Yellowstone National Park. These strains are closely related to predominant filamentous anoxygenic phototrophs found in the mat, as judged by the similarity of small-subunit rRNA, lipid distributions, and genomic and metagenomic sequences. Like a Japanese isolate, R. castenholzii, the Yellowstone isolates contain bacteriochlorophyll a, but not bacteriochlorophyll c or chlorosomes, and grow photoheterotrophically or chemoheterotrophically under dark aerobic conditions. The genome of one isolate, Roseiflexus sp. strain RS1, contains genes necessary to support these metabolisms. This genome also contains genes encoding the 3-hydroxypropionate pathway for CO2 fixation and a hydrogenase, which might enable photoautotrophic metabolism, even though neither isolate could be grown photoautotrophically with H2 or H2S as a possible electron donor. The isolates exhibit temperature, pH, and sulfide preferences typical of their habitat. Lipids produced by these isolates matched much better with mat lipids than do lipids produced by R. castenholzii or Chloroflexus isolates.

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