scholarly journals Analysis of the retinoic acid receptor-α (RARα/Rara) dependent transcriptome in F9 embryonal carcinoma cells

2015 ◽  
Author(s):  
KB Laursen
Keyword(s):  
Retinoic Acid ◽  
Embryonal Carcinoma ◽  
Retinoic Acid Receptor ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Acid Receptor ◽  
F9 Embryonal Carcinoma Cells ◽  
Retinoic Acid Receptor Α

scholarly journals Specific and Redundant Functions of Retinoid X Receptor/Retinoic Acid Receptor Heterodimers in Differentiation, Proliferation, and Apoptosis of F9 Embryonal Carcinoma Cells

1997 ◽  
Vol 139 (3) ◽  
pp. 735-747 ◽  
Author(s):  
Hideki Chiba ◽  
John Clifford ◽  
Daniel Metzger ◽  
Pierre Chambon
Keyword(s):  
Retinoic Acid ◽  
Embryonal Carcinoma ◽  
Retinoic Acid Receptor ◽  
Retinoid X Receptor ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Acid Receptor ◽  
Proliferation And Apoptosis ◽  
Redundant Functions

We have generated F9 murine embryonal carcinoma cells in which either the retinoid X receptor (RXR)α and retinoic acid receptor (RAR)α genes or the RXRα and RARγ genes are knocked out, and compared their phenotypes with those of wild-type (WT), RXRα−/−, RARα−/−, and RARγ−/− cells. RXRα−/−/ RARα−/− cells were resistant to retinoic acid treatment for the induction of primitive and parietal endodermal differentiation, as well as for antiproliferative and apoptotic responses, whereas they could differentiate into visceral endodermlike cells, as previously observed for RXRα−/− cells. In contrast, RXRα−/−/RARγ−/− cells were defective for all three types of differentiation, as well as antiproliferative and apoptotic responses, indicating that RXRα and RARγ represent an essential receptor pair for these responses. Taken together with results obtained by treatment of WT and mutant F9 cells with RAR isotype– and panRXR-selective retinoids, our observations support the conclusion that RXR/ RAR heterodimers are the functional units mediating the retinoid signal in vivo. Our results also indicate that the various heterodimers can exert both specific and redundant functions in differentiation, proliferation, and apoptosis. We also show that the functional redundancy exhibited between RXR isotypes and between RAR isotypes in cellular processes can be artifactually generated by gene knockouts. The present approach for multiple gene targeting should allow inactivation of any set of genes in a given cell.

Aggregation and cell cycle dependent retinoic acid receptor mRNA expression in P19 embryonal carcinoma cells

1992 ◽  
Vol 36 (3) ◽  
pp. 165-172 ◽  
Author(s):  
Luigi J.C. Jonk ◽  
Marjolijn E.J. de Jonge ◽  
Frank A.E. Kruyt ◽  
Christine L. Mummery ◽  
Paul T. van der Saag ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Retinoic Acid ◽  
Mrna Expression ◽  
Embryonal Carcinoma ◽  
Retinoic Acid Receptor ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Receptor Mrna ◽  
P19 Embryonal Carcinoma Cells ◽  
Cycle Dependent
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