scholarly journals Protein sequence analysis based on hydropathy profile of amino acids

2012 ◽  
Vol 13 (2) ◽  
pp. 152-158 ◽  
Author(s):  
Xiao-li Xie ◽  
Li-fei Zheng ◽  
Ying Yu ◽  
Li-ping Liang ◽  
Man-cai Guo ◽  
...  
Keyword(s):  
Amino Acids ◽  
Sequence Analysis ◽  
Protein Sequence ◽  
Protein Sequence Analysis

Bioinformatics Analysis and Characteristics of US2 Protein Encoded by the Newly Identified US2 Gene of Duck Enteritis Virus

2012 ◽  
Vol 424-425 ◽  
pp. 493-497
Author(s):  
Jie Gao ◽  
An Chun Cheng ◽  
Ming Shu Wang
Keyword(s):  
Amino Acids ◽  
Sequence Analysis ◽  
Protein Sequence ◽  
Bioinformatics Analysis ◽  
Functional Study ◽  
Protein Sequence Analysis ◽  
Tegument Proteins ◽  
The Us ◽  
New Type ◽  
Enteritis Virus

In this article,we intend to provide readers with some bioinformation of DEV Us2 protein,for little information of this protein has been cited online.Us2 protein encoded by the DEV-US2 gene is one of the DEV tegument proteins. Like most members of the alphaherpesvirinae,the genes in the US region of its genome are well conserved,which means that both the major DEV protein Us2 and alphaherpesvirinae protein Us2 possess the similar functions.Us2 protein sequence analysis indicates that the protein possesses typical characteristics of tegument protein.The precursor Us2 consists of 239 amino acids and exhibits a molecular mass of 34 kDa. In conclusion,all the datas and consequences will provide a basis for further functional study of the DEV-Us2 protein and provide necessary datas for the new type clinical diagnosis of DEV and the development of new DEV vaccine.

scholarly journals A novel manual method for protein-sequence analysis

1976 ◽  
Vol 157 (1) ◽  
pp. 77-85 ◽  
Author(s):  
J Y Chang ◽  
E H Creaser
Keyword(s):  
Amino Acids ◽  
Sequence Analysis ◽  
Protein Sequence ◽  
Detection Sensitivity ◽  
Phenyl Isothiocyanate ◽  
Protein Sequence Analysis ◽  
Manual Method ◽  
Terminal Residue ◽  
A Chain

A novel manual method for protein-sequence analysis is described. Three peptides, the hexapeptide (Leu-TRP-Met-Arg-Phe-Ala), insulin A chain and glucagon were used to test this technique. Peptides (1 or 2 nmol) were hydrolysed with acid and their qualitative amino acid compositions were confirmed by reacting with 4-NN-dimethylaminoazobenzene-4'-sulphonylchloride and 4-NN-dimethylaminoazobenzene 4'-isothiocyanate. Sequence determination of 20-200 nmol of peptide was then performed by the combined use of phenyl isothiocyanate and 4-NN-dimethylaminoazobenzene 4'-isothiocyanate, a new procedure that is analogous to the dansyl-Edman method with the replacement of dansyl chloride by 4-NN-dimethylaminoazobenzene 4'-isothiocyanate as the N-terminal residue determination reagent. On t.l.c. this new N-terminal reagent gave brightly coloured 4-NN-dimethylaminoazobenzene-4-thiohydantoins of amino acids and showed the following advantages: (1) the detection sensitivity is in the pmol range; (2) u.v. observation is not required; (3) there is no destruction of acid-labile amino acids; (4) two-dimensional t.l.c. separation is adequate to identify 24 amino acids, except leucine and isoleucine (this pair of amino acids can be resolved by using 4-NN-dimethylaminoazobenzene-4'-sulphonyl chloride); (5) the determination of a new N-terminal residue (from coupling to t.l.c. identification) takes only 3 h; (6) the colour difference beteen isothiocyanate, thiocarbamoyl and thiohydantoin derivatives facilitates the identifications.

Chemical Methods for Protein Sequence Analysis

2007 ◽  
pp. 141-158
Author(s):  
Friedrich Lottspeich ◽  
Tony Houthaeve ◽  
Roland Kellner
Keyword(s):  
Sequence Analysis ◽  
Protein Sequence ◽  
Protein Sequence Analysis ◽  
Chemical Methods
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