Transfer of Borrelia Burgdorferi s.s. Infection via Blood Transfusion in a Murine Model

2006 ◽  
Vol 92 (4) ◽  
pp. 869-870 ◽  
Author(s):  
Elizabeth S. Gabitzsch ◽  
Joseph Piesman ◽  
Marc C. Dolan ◽  
Christine M. Sykes ◽  
Nordin S. Zeidner
Keyword(s):  
Blood Transfusion ◽  
Borrelia Burgdorferi ◽  
Murine Model

scholarly journals Efficacy of an Evernimicin (SCH27899) In Vitro and in an Animal Model of Lyme Disease

2001 ◽  
Vol 45 (3) ◽  
pp. 936-937 ◽  
Author(s):  
Charles S. Pavia ◽  
Gary P. Wormser ◽  
John Nowakowski ◽  
Anthony Cacciapuoti
Keyword(s):  
Animal Model ◽  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Antibiotic Treatment ◽  
Murine Model ◽  
C3h Mice

ABSTRACT The MICs of evernimicin at which 90% of Borrelia burgdorferi patient isolates were inhibited ranged from 0.1 to 0.5 μg/ml. Evernimicin was as effective as ceftriaxone againstB. burgdorferi in a murine model of experimental Lyme disease. As assessed by culturing the urinary bladders of infected C3H mice, no live Borrelia isolates were recoverable following antibiotic treatment.

Lack of transmission of Borrelia burgdorferi by blood transfusion

The Lancet ◽  
1990 ◽  
Vol 335 (8688) ◽  
pp. 550 ◽  
Author(s):  
Lars Halkier-Sørensen ◽  
Knud Kragballe ◽  
SteenT. Nedergaard ◽  
Jan Jørgensen ◽  
Klaus Hansen
Keyword(s):  
Blood Transfusion ◽  
Borrelia Burgdorferi

scholarly journals Analysis of Mechanisms Associated with Loss of Infectivity of Clonal Populations of Borrelia burgdorferi B31MI

2001 ◽  
Vol 69 (6) ◽  
pp. 3670-3677 ◽  
Author(s):  
John V. McDowell ◽  
Shian Ying Sung ◽  
Maria Labandeira-Rey ◽  
Jon T. Skare ◽  
Richard T. Marconi
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Murine Model ◽  
Southern Hybridization ◽  
Clonal Population ◽  
Suggestive Evidence

ABSTRACT Numerous studies have provided suggestive evidence that the loss of plasmids correlates with the loss of infectivity of the Lyme disease spirochetes. In this study we have further investigated this correlation. Clonal populations were obtained from the skin of a mouse infected for 3 months with a clonal population of Borrelia burgdorferi B31MI. The complete plasmid compositions of these populations were determined using a combination of PCR and Southern hybridization. The infectivities of clones differing in plasmid composition were tested using the C3H-HeJ murine model for Lyme disease. While several clones were found to be noninfectious, a correlation between the loss of a specific plasmid and loss of infectivity in the clones analyzed in this report was not observed. While it is clear from recent studies that the loss of some specific plasmids results in attenuated virulence, this study demonstrates that additional mechanisms also contribute to the loss of infectivity.

scholarly journals Babesia microti—Borrelia Burgdorferi Coinfection

Pathogens ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 117 ◽  
Author(s):  
Nikhat Parveen ◽  
Purnima Bhanot
Keyword(s):  
Lyme Disease ◽  
Blood Transfusion ◽  
Borrelia Burgdorferi ◽  
Geographic Distribution ◽  
Causative Agent ◽  
Ixodes Scapularis ◽  
Protozoan Parasite ◽  
Babesia Microti ◽  
Human Babesiosis ◽  
The U.S

The incidence and geographic distribution of human babesiosis is growing in the U.S. Its major causative agent is the protozoan parasite, Babesia microti. B. microti is transmitted to humans primarily through the bite of Ixodes scapularis ticks, which are vectors for a number of other pathogens. Other routes of B. microti transmission are blood transfusion and in rare cases of mother-to-foetus transmission, through the placenta. This review discusses the current literature on mammalian coinfection with B. microti and Borrelia burgdorferi, the causative agent Lyme disease.

scholarly journals DETERMINING AN APPROPRIATE MURINE MODEL FOR STUDYING THE PATHOLOGICAL RESPONSE TO BORRELIA BURGDORFERI IN DEVELOPING LYME CARDITIS

2021 ◽  
Vol 77 (18) ◽  
pp. 652
Author(s):  
Veronica N. Harrison ◽  
Catherine Brissette ◽  
Timothy Casselli ◽  
Derick Thompson ◽  
Heidi Pecoraro ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Murine Model ◽  
Pathological Response ◽  
Lyme Carditis

An rfuABCD -like operon and its relationship to riboflavin utilization and mammalian

2021 ◽  
Author(s):  
Matthew K. Muramatsu ◽  
Jianli Zhou ◽  
Bryna L. Fitzgerald ◽  
Ranjit K. Deka ◽  
John T. Belisle ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Lyme Borreliosis ◽  
Murine Model ◽  
Flavin Adenine Dinucleotide ◽  
Antimicrobial Effect ◽  
Flavin Mononucleotide

Riboflavin is an essential micronutrient, but its transport and utilization has remained largely understudied among pathogenic spirochetes. Here we show that Borrelia burgdorferi , the zoonotic spirochete that causes Lyme disease, is able to import riboflavin via products of its rfuABCD -like operon as well as synthesize flavin mononucleotide and flavin adenine dinucleotide despite lacking canonical genes for their synthesis. Additionally, a mutant deficient in the rfuABCD -like operon is resistant to the antimicrobial effect of roseoflavin, a natural riboflavin analog, and is attenuated in a murine model of Lyme borreliosis. Our combined results indicate that not only are riboflavin and the maintenance of flavin pools essential for B. burgdorferi growth, but that flavin utilization and its downstream products (e.g., flavoproteins) may play a more prominent role in B. burgdorferi pathogenesis than previously appreciated.

scholarly journals Decorin-Binding Protein A (DbpA) of Borrelia burgdorferi Is Not Protective When Immunized Mice Are Challenged via Tick Infestation and Correlates with the Lack of DbpA Expression by B. burgdorferi in Ticks

2000 ◽  
Vol 68 (8) ◽  
pp. 4759-4764 ◽  
Author(s):  
Kayla E. Hagman ◽  
Xiaofeng Yang ◽  
Stephen K. Wikel ◽  
George B. Schoeler ◽  
Melissa J. Caimano ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Murine Model ◽  
Blood Meal ◽  
Tick Infestation ◽  
Ixodes Scapularis ◽  
Binding Protein ◽  
Protein A ◽  
Lyme Disease Vaccine

ABSTRACT Previous studies showed that decorin-binding protein A (DbpA) ofBorrelia burgdorferi was a protective immunogen in the murine model of Lyme borreliosis when mice were challenged (needle inoculated) intradermally with in vitro-cultivated spirochetes. In the present study, DbpA-immunized C3H/HeJ mice were not protected from infection when infested with Ixodes scapularis nymphs harboring virulent B. burgdorferi 297. This lack of protection correlated with the failure to detect DbpA on B. burgdorferi in ticks, suggesting that DbpA is not available as a target for bactericidal antibodies in serum when B. burgdorferi-infected ticks take their blood meal from an immunized host. The failure of DbpA immunization to protect tick-challenged mice contradicts the results of earlier needle inoculation vaccination experiments and suggests that DbpA may not be suitable as a Lyme disease vaccine.

scholarly journals Variations in Barbour-Stoenner-Kelly Culture Medium Modulate Infectivity and Pathogenicity of Borrelia burgdorferi Clinical Isolates

2004 ◽  
Vol 72 (11) ◽  
pp. 6702-6706 ◽  
Author(s):  
Guiqing Wang ◽  
Radha Iyer ◽  
Susan Bittker ◽  
Denise Cooper ◽  
Jennifer Small ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Prospective Study ◽  
Clinical Isolate ◽  
Murine Model ◽  
Prospective Studies ◽  
Culture Medium ◽  
Clinical Isolates ◽  
In Vitro Cultivation ◽  
A Prospective Study

ABSTRACT The effects of variations in Barbour-Stoenner-Kelly (BSK) medium on the infectivity and pathogenicity of Borrelia burgdorferi clinical isolates were assessed by retrospective and prospective studies using a murine model of Lyme borreliosis. Thirty of 35 (86%) mice infected with any of six virulent B. burgdorferi clinical isolates grown in a BSK-H medium developed clinically apparent arthritis. By contrast, arthritis was observed in only 25 of 60 (42%) mice inoculated with two of these B. burgdorferi strains grown in a different lot of BSK-H medium (P < 0.001). In a prospective study, mice inoculated with a B. burgdorferi clinical isolate grown in a BSK medium prepared in-house produced significantly greater disease than those injected with the same isolate cultured in BSK-H medium (P < 0.05). The attenuated pathogenicity is not due to the loss of plasmids during in vitro cultivation. The data suggest that variations in BSK medium have a significant impact on the infectivity and pathogenicity of B. burgdorferi clinical isolates.

scholarly journals Efficacy of an Experimental Azithromycin Cream for Prophylaxis of Tick-Transmitted Lyme Disease Spirochete Infection in a Murine Model

2013 ◽  
Vol 58 (1) ◽  
pp. 348-351 ◽  
Author(s):  
Joseph Piesman ◽  
Andrias Hojgaard ◽  
Amy J. Ullmann ◽  
Marc C. Dolan
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Murine Model ◽  
Systemic Effect ◽  
Tick Bite ◽  
Single Application ◽  
Transmitted Infection ◽  
Content Type ◽  
Tick Transmission ◽  
A Site

ABSTRACTAs an alternative to oral prophylaxis for the prevention of tick transmission ofBorrelia burgdorferi, we tested antibiotic cream prophylactic formulations in a murine model of spirochete infection. A 4% preparation of doxycycline cream afforded no protection, but a single application of 4% azithromycin cream was 100% protective when applied directly to the tick bite site at the time of tick removal. Indeed, the azithromycin cream was 100% effective when applied at up to 3 days after tick removal and protected 74% of mice exposed to tick bite when applied at up to 2 weeks after tick removal. Azithromycin cream was also protective when applied at a site distal to the tick bite site, suggesting that it was having a systemic effect in addition to a local transdermal effect. Mice that were protected from tick-transmitted infection did not seroconvert and did not infect larval ticks on xenodiagnosis. Azithromycin cream formulations appear to hold promise for Lyme disease prophylaxis.

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