NEUTRAL STEROID SULPHATES IN AMNIOTIC FLUID

1970 ◽  
Vol 46 (3) ◽  
pp. 391-399 ◽  
Author(s):  
T. LUUKKAINEN ◽  
A. SIEGEL ◽  
R. VIHKO

SUMMARY Steroid monosulphate and disulphate fractions were obtained by chromatography on Sephadex LH-20 from human amniotic fluid collected at term. Only 16α-hydroxydehydroepiandrosterone could be identified in the monosulphate fraction, the detection limit being 5–8 μg./1000 ml. amniotic fluid. In the disulphate fraction 5-androstene-3β,17α-diol, 5-androstene-3β,17β-diol, 5-pregnene-3β,20α-diol, 5α-pregnane-3β,20α-diol, 16α-hydroxydehy-droepiandrosterone, 16β-hydroxydehydroepiandrosterone, 3β, 17β-dihy-droxy-5-androsten-16-one and 5-pregnene-3β,17α,20α-triol were identified by gas—liquid chromatography and gas chromatography—mass spectrometry. The concentrations of these steroids were determined in a pool of human amniotic fluid taken at term. The main compound was 5-androstene-3β,17α-diol.

1971 ◽  
Vol 51 (1) ◽  
pp. 109-115 ◽  
Author(s):  
T. LUUKKAINEN ◽  
E. A. MICHIE ◽  
R. VIHKO

SUMMARY Steroid disulphate fractions were obtained by chromatography on Sephadex LH-20 from samples of amniotic fluid from three pregnancies complicated by anencephaly. Several of the steroids with a 3β-hydroxy-5-ene structure found in normal amniotic fluid could not be detected. 5-Androstene-3β,17α-diol, the main steroid disulphate in normal amniotic fluid was present in concentrations of <5% of the values at normal term. The disulphates of neutral steroids found in highest concentrations in the amniotic fluid of anencephalic pregnancies were those of isomeric pregnanediols; 5α-pregnane-3α,20α-diol, 5α-pregnane-3β,20α-diol and 5β-pregnane-3α,20α-diol. The quantities of these metabolites of progesterone in the amniotic fluid of anencephalic pregnancies were similar to those in normal pregnancies. A saturated 18-hydroxylated C19 steroid, most probably 3α,18-dihydroxy-5α-androstan-17-one, was identified by gas—liquid chromatography and gas chromatography—mass spectrometry. Although this compound has been previously identified in human bile and in normal pregnancy urine, this appears to be the first identification of a saturated C19 steroid in amniotic fluid.


1976 ◽  
Vol 22 (6) ◽  
pp. 915-921 ◽  
Author(s):  
G F Johnson ◽  
C J Least ◽  
J W Serum ◽  
E B Solow ◽  
H M Solomon

Abstract We describe a case of fatal overdosage with primidone and methsuximide. During the early phase of the patient's hospital course we found concentrations of methsuximide, N-desmethylmethsuximide, and primidone in serum that far exceeded the usual therapeutic concentrations, as determined by gas-liquid chromatography. Determination of N-desmethylmethsuximide in peritoneal fluid demonstrated concentrations comparable to those in serum. This led to the therapeutic decision to manage the patient by dialysis. Subsequently, serum samples collected during the course of hospitalization were analyzed quantitatively by gas-liquid chromatography for methsuximide, N-desmethylmethsuximide, primidone, phenobarbital, and diphenylhydantoin. Selected serum specimens were also analyzed by gas chromatography-mass spectrometry, and N-methyl-2-hydroxymethyl-2-phenylsuccinimide, a metabolite of methsuximide not previously described in human serum, was identified by analysis of its mass spectrum.


1972 ◽  
Vol 180 (1059) ◽  
pp. 179-186 ◽  

Gas—liquid chromatography and gas chromatography—mass spectrometry have been used to study the pattern of sterols in meconium and faeces from newborn infants. Meconium contained metabolites from various steps in the biosynthesis of cholesterol, e. g. lanosterol, 24, 25-dihydro-∆ 8 -lanosterol, 24, 25-dihydro-∆ 9 -lanosterol, 4 α ,14 α -dimethyl-5 α -cholest-7 (and 8)-en-3 β -ol, 4, 4-dimethyl-5 α -cholest-8-en-3 β -ol, 4, 4-dimethyl-5 α -cholesta-8, 14-dien-3 β -ol and ∆ 7 - and ∆ 8 -methostenol. Meconium and faeces from newborns also contained the sulphate esters of 20, 22-dihydroxycholesterol and of 22-, 23- and 24-hydroxycholesterol.


1981 ◽  
Vol 64 (1) ◽  
pp. 186-190
Author(s):  
Glenn E Martin ◽  
James M Burggraff ◽  
Randolph H Dyer ◽  
Peter C Buscemi

Abstract A procedure is described for the separation and determination of the following compounds which are found as congeners in alcoholic products: acetaldehyde, ethyl acetate, methanol, ethanol, n-propanol, isobutanol, n-butanol, acetic acid, and the amyl alcohols (2-methyl-l-butanol and 3-methyl-l-butanol). These compounds were separated by gas-liquid chromatography (GLC) using 5% Carbowax 20M on Carbopack B as the column packing. The results obtained by using this method were compared with those obtained using the present AOAC methods for these compounds. GLC chromatograms show better resolution by the method presented.


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