Time-resolved fluoroimmunoassay of plasma daidzein and genistein11Abbreviations: Daidzein (Da), genistein (Gen), gas-liquid chromatography (GC), high performance liquid chromatography (HPLC), mass spectrometry (MS), radioimmunoassay (RIA), time-resolved fluoroimmunoassay (TR-FIA), enzymeimmunoassay (EIA), fluoroimmunoassay (FIA), bovine serum albumin (BSA), N,N′-dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS), isotope dilution gas chromatography-mass spectrometry in the selected ion monitoring mode (ID-GC-MS-SIM), coefficients of variation (CVs). B = Binding of tracer to antibody in the presence of unlabeled analyte. B0 = Binding of tracer to antibody in the absence of unlabeled analyte. T = Total amount of tracer added. Estradiol = estradiol-17β.

Steroids ◽  
2000 ◽  
Vol 65 (6) ◽  
pp. 339-348 ◽  
Author(s):  
Guojie J Wang ◽  
Oldrich Lapcı́k ◽  
Richard Hampl ◽  
Mariko Uehara ◽  
Nawaf Al–Maharik ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Gas Chromatography Mass Spectrometry ◽  
Gas Liquid Chromatography ◽  
Selected Ion Monitoring ◽  
Time Resolved ◽  
Coefficients Of Variation ◽  
Estradiol 17Β

Monitoring drug concentrations in a case of combined overdosage with primidone and methsuximide.

1976 ◽  
Vol 22 (6) ◽  
pp. 915-921 ◽  
Author(s):  
G F Johnson ◽  
C J Least ◽  
J W Serum ◽  
E B Solow ◽  
H M Solomon
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Gas Liquid Chromatography ◽  
Therapeutic Decision ◽  
Serum Samples ◽  
Monitoring Drug ◽  
Drug Concentrations

Abstract We describe a case of fatal overdosage with primidone and methsuximide. During the early phase of the patient's hospital course we found concentrations of methsuximide, N-desmethylmethsuximide, and primidone in serum that far exceeded the usual therapeutic concentrations, as determined by gas-liquid chromatography. Determination of N-desmethylmethsuximide in peritoneal fluid demonstrated concentrations comparable to those in serum. This led to the therapeutic decision to manage the patient by dialysis. Subsequently, serum samples collected during the course of hospitalization were analyzed quantitatively by gas-liquid chromatography for methsuximide, N-desmethylmethsuximide, primidone, phenobarbital, and diphenylhydantoin. Selected serum specimens were also analyzed by gas chromatography-mass spectrometry, and N-methyl-2-hydroxymethyl-2-phenylsuccinimide, a metabolite of methsuximide not previously described in human serum, was identified by analysis of its mass spectrum.

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