SIMULTANEOUS RADIOIMMUNOASSAY OF LUTEINIZING HORMONE AND FOLLICLE-STIMULATING HORMONE

1978 ◽  
Vol 79 (3) ◽  
pp. 407-408 ◽  
Author(s):  
M. J. ELLIS ◽  
R. A. DONALD ◽  
J. H. LIVESEY
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Significant Proportion ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Operating Costs ◽  
Two Factors ◽  
And Performance ◽  
Time Required ◽  
Stimulating Hormone

The Medical Unit, The Princess Margaret Hospital, Christchurch 2, New Zealand (Revised manuscript received 21 August 1978) The frequent clinical and research requirement for measurement of both plasma luteinizing hormone (LH) and follicle-stimulating hormone (FSH) has prompted the development of a simultaneous radioimmunoassay for these two hormones. The considerable advantages of a simultaneous method include an economy of plasma volume, assay reagents, test-tubes and, more importantly, the time required for radioactive counting and performance of the assay by technical staff. The latter two factors comprise a significant proportion of radioimmunoassay operating costs. This report describes a simultaneous radioimmunoassay based on the use of 131I-labelled FSH, 125I-labelled LH, anti-FSH serum M93 6873 (a generous gift from Professor W. R. Butt, Birmingham), anti-human chorionic gonadotrophin (HCG) serum for LH measurement (Donald, 1972) and donkey anti-rabbit precipitating serum (Wellcome Reagents, U.K.) for separation of antibody-bound and free hormones. Pituitary gonadotrophin standard (LER 907)

ANTIGONADOTROPHIC PROFILE OF ANTISERA AGAINST HUMAN GONADOTROPHIN PREPARATIONS

1971 ◽  
Vol 67 (2) ◽  
pp. 262-276 ◽  
Author(s):  
P. Petrusz ◽  
C. Robyn ◽  
E. Diczfalusy
Keyword(s):  
Biological Activity ◽  
Luteinizing Hormone ◽  
Total Dose ◽  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Immunological Activity ◽  
Human Menopausal Gonadotrophin ◽  
Stimulating Hormone

ABSTRACT Forty-two antisera were prepared in rabbits against human chorionic gonadotrophin (HCG), human hypophysial gonadotrophin (HHG), human urinary luteinizing hormone (LH) and human menopausal gonadotrophin (HMG) preparations. The gonadotrophic profiles of the antigens were previously characterized by bioassay, immunoassay and bioimmunoassay methods. The 25 most potent antisera were tested in statistically valid bioassays for their HCG and follicle stimulating hormone (FSH) neutralizing activities as well as for their neutralizing potencies against the FSH-like activity present in HCG preparations. The anti-HCG/anti-FSH ratios of the anti-HCG sera tested varied between 6.2 and > 254, while those of the anti-HHG, anti-LH and anti-HMG sera were close to 2. It was found that the total dose of immunological activity (anti-HCG neutralizing and anti-FSH neutralizing potency) rather than that of the biological activity administered to the rabbits was decisive for obtaining antisera with high anti-HCG and anti-FSH titers. Immunization with a highly purified HCG preparation (> 17 000 IU/mg) resulted in antisera exhibiting lower anti-HCG/anti-FSH ratios than did immunization with partially purified preparations. A highly purified urinary LH preparation which did not contain any detectable FSH activity gave rise to antisera exhibiting anti-HCG/anti-FSH ratios of approximately 2.0. These highly purified HCG and LH preparations were shown previously to possess high anti-FSH neutralizing potencies (Petrusz et al. 1971b). Booster injections did not change significantly the quality or the titer of the antigonadotrophic sera studied. The HCG neutralizing potency of anti-HCG sera was approximately 3 times higher when assayed against a highly purified HCG preparation (> 17 000 IU/mg) as compared to potency estimates obtained against the laboratory standard of HCG (about 2000 IU/mg). It is suggested that consideration should be given to the establishment of standard preparations of antigonadotrophic sera. It is concluded that bioimmunoassays are more suitably than conventional bioassay methods for the assessment of the antigenic purity of human gonadotrophin preparations.

PRODUCTION OF ANTISERA AGAINST HIGHLY PURIFIED HUMAN FOLLICLE-STIMULATING HORMONE, LUTEINIZING HORMONE AND THYROID-STIMULATING HORMONE

1976 ◽  
Vol 70 (3) ◽  
pp. 335-344 ◽  
Author(s):  
J. I. THORELL ◽  
B. HOLMSTRÖM
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Thyroid Stimulating Hormone ◽  
High Titre ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Antigenic Determinants ◽  
High Avidity ◽  
Cross Reactions ◽  
Stimulating Hormone

SUMMARY Antisera were produced in rabbits against highly purified preparations of human LH (2000 or 10000 i.u./mg), human FSH (5500 i.u./mg), and human TSH (7·5 i.u./mg). Most rabbits produced antisera of high titre and high avidity. Cross-reactions were minimal between human TSH and human chorionic gonadotrophin (HCG) and between human FSH and HCG but marked between human LH and HCG. TSH and FSH also showed a constant but relatively weak cross-reaction. LH cross-reacted with FSH to a higher degree than did HCG. The avidity of the antisera was high. It was concluded that much of the lack of specificity recorded for glycoprotein antisera are effects of impure immunogens. Some of the true cross-reactions are probably explained by shared antigenic determinants of the β-subunits. Unadsorbed antisera could be used for assay of FSH and TSH in plasma from pregnant women.

SELECTIVE RELEASE OF FOLLICLE-STIMULATING HORMONE DURING THE PERIOD OF OVULATION INDUCED BY HUMAN CHORIONIC GONADOTROPHIN IN DIOESTROUS RATS

1977 ◽  
Vol 75 (1) ◽  
pp. 179-180 ◽  
Author(s):  
SHUJI SASAMOTO ◽  
SHIGEO HARADA ◽  
KAZUYOSHI TAYA
Keyword(s):  
Luteinizing Hormone ◽  
Wistar Rats ◽  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Present Communication ◽  
Follicular Maturation ◽  
Female Wistar Rats ◽  
Premature Ovulation ◽  
Stimulating Hormone

Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183, Japan (Received 2 May 1977) When an amount of human chorionic gonadotrophin (HCG) sufficient to cause ovulation is given to 4-day cyclic rats on the day of dioestrus, premature ovulation is induced the next morning (Eto & Imamichi, 1955). The pattern of release of follicle-stimulating hormone (FSH) responsible for the initiation of follicular maturation of the next set of follicles (Schwartz, 1969; Welschen & Dullaart, 1976) after HCG-induced ovulation has not been previously evaluated. The present communication is concerned with this problem and indicates that a large amount of FSH is released within 12 h of administration of HCG, with only a small concomitant rise in the concentration of luteinizing hormone (LH). Adult female Wistar rats were maintained under a 14 h light : 10 h darkness schedule (lights on 05.00 h), and those showing three or

CHANGES IN THE BINDING OF HUMAN CHORIONIC GONADOTROPHIN/LUTEINIZING HORMONE, FOLLICLE-STIMULATING HORMONE AND PROLACTIN TO HUMAN CORPORA LUTEA DURING THE MENSTRUAL CYCLE AND PREGNANCY

1980 ◽  
Vol 87 (3) ◽  
pp. 315-325 ◽  
Author(s):  
A. S. McNEILLY ◽  
J. KERIN ◽  
I. A. SWANSTON ◽  
T. A. BRAMLEY ◽  
D. T. BAIRD
Keyword(s):  
Menstrual Cycle ◽  
Luteinizing Hormone ◽  
Luteal Phase ◽  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Corpora Lutea ◽  
Maximum Serum ◽  
Human Menstrual Cycle ◽  
Stimulating Hormone

The changes in the binding of human chorionic gonadotrophin/luteinizing hormone (HCG/LH), follicle-stimulating hormone (FSH) and prolactin to 44 corpora lutea have been assessed during the luteal phase of the human menstrual cycle and early pregnancy. All corpora lutea bound HCG but out of 32 only ten bound FSH and only seven bound prolactin specifically. While binding of HCG increased to maximal levels in the mid-luteal phase, binding of FSH and prolactin was most often found in the early luteal phase. Maximum binding of HCG was associated with maximum serum levels of progesterone. Luteal regression was associated with a decrease in the binding of HCG but a causal relationship could not be established. Very low binding of HCG was found to corpora lutea of pregnancy. These results show that (1) the changes in binding of HCG during the luteal phase of the human menstrual cycle are similar to those in other species and (2) there are specific binding sites for prolactin and FSH in the human corpus luteum.

THE EFFECT OF 6 m UREA ON THE FOLLICLE-STIMULATING AND LUTEINIZING HORMONE ACTIVITIES OF VARIOUS GONADOTROPHIN PREPARATIONS

1962 ◽  
Vol 24 (2) ◽  
pp. 153-158 ◽  
Author(s):  
H. SCHMIDT-ELMENDORFF ◽  
J. A. LORAINE ◽  
E. T. BELL
Keyword(s):  
Ascorbic Acid ◽  
Biological Activity ◽  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Considerable Reduction ◽  
Mouse Uterus ◽  
Stimulating Hormone

SUMMARY The luteinizing hormone (LH), follicle-stimulating hormone (FSH) and 'total gonadotrophic' activities of various hormones have been studied following incubation with 6 m urea at 40° c for 24 hr. LH activity was estimated by the ovarian ascorbic acid depletion test in rats, FSH activity by the augmentation test in mice, and 'total gonadotrophic' activity by the mouse uterus test. Following incubation with 6 m urea the LH activities of NIH—LH, NIH—FSH, human chorionic gonadotrophin and Pergonal were almost completely destroyed, while the LH activity of pregnant mares' serum gonadotrophin (PMSG) was reduced to a smaller extent. The FSH activity of NIH—FSH was little affected by this form of treatment, but in the case of Pergonal a considerable reduction of FSH activity occurred. The 'total gonadotrophic' activity of NIH—FSH, PMSG and Pergonal was reduced after incubation with 6 m urea, the degree of inactivation being greatest in the case of Pergonal. After control incubations with water no appreciable loss of biological activity was observed with any hormone other than Pergonal.

scholarly journals Treatment of immature mice with gonadotrophins. The influence of mouse age on the response of ovarian alkaline phosphatase activities to gonadotrophins

1975 ◽  
Vol 152 (2) ◽  
pp. 365-372 ◽  
Author(s):  
Thomas A. Bramley
Keyword(s):  
Alkaline Phosphatase ◽  
Luteinizing Hormone ◽  
Alkaline Phosphatase Activity ◽  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Uterine Weight ◽  
Human Pituitary ◽  
Serum Luteinizing Hormone ◽  
Stimulating Hormone

Treatment of mice aged 23–25 days with chorionic gonadotrophin induced large amounts of an ovarian alkaline phosphatase activity (phosphatase Ib) kinetically distinct from that of untreated ovaries (phosphatase I). The activities of alkaline phosphatase I and Ib varied with age in untreated mice. Phosphatase Ib appeared when serum luteinizing hormone concentrations increased (days 4–10 and days 35–45), and disappeared when concentrations were low (days 11–35). Injection of human chorionic gonadotrophin induced progressively larger amounts of phosphatase Ib activity between day 19 and day 29. However, gonadotrophin treatment failed to induce this activity on days 10–18 and 30–35. Nevertheless, during the latter period, human chorionic gonadotrophin induced especially large increases in uterine weight. Treatment at different ages with sheep luteinizing hormone plus human pituitary follicle-stimulating hormone induced a pattern of response identical with that induced by human chorionic gonadotrophin, although sheep luteinizing hormone alone was ineffective before 35 days. In contrast, human luteinizing hormone induced a response in the absence of exogenous follicle-stimulating hormone.

RESPONSE OF MOUSE GRAAFIAN FOLLICLES IN ORGAN CULTURE TO VARYING DOSES OF FOLLICLE-STIMULATING HORMONE AND LUTEINIZING HORMONE

1975 ◽  
Vol 65 (1) ◽  
pp. 27-32 ◽  
Author(s):  
P. NEAL ◽  
T. G. BAKER
Keyword(s):  
Luteinizing Hormone ◽  
Organ Culture ◽  
Oocyte Maturation ◽  
Mode Of Action ◽  
Follicle Stimulating Hormone ◽  
Ovarian Response ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Stimulating Hormone

SUMMARY The response of mouse ovaries maintained in organ culture to follicle-stimulating hormone (FSH), luteinizing hormone (LH) and human chorionic gonadotrophin (HCG) was assessed using quantitative histological and radioimmunoassay techniques. In terms of the induction of preovulatory maturation in follicular oocytes, 1 μg FSH/ml medium was as effective as 10 μg LH/ml. The lowest doses of HCG and LH used (0·2 i.u./ml and 1 μg/ml respectively) had no effect on oocyte maturation, whereas the response to FSH was virtually unchanged irrespective of dose (1–10 μg/ml). When the level of progesterone in the medium at the end of organ culture was used as an index of ovarian response, LH was more effective than FSH and HCG, although all the hormones induced a significant increase, irrespective of dose. These results are discussed in terms of the mode of action of gonadotrophins in the processes culminating in ovulation.

Therapeutic use of gonadotrophins and clomiphene

1969 ◽  
Vol 7 (9) ◽  
pp. 33-35
Keyword(s):  
Pregnant Women ◽  
Follicle Stimulating Hormone ◽  
Therapeutic Use ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Human Pituitary ◽  
Synthetic Compound ◽  
Pituitary Glands ◽  
Post Menopausal ◽  
Stimulating Hormone

The three substances now used to stimulate the gonads in infertility are human follicle stimulating hormone (HFSH) obtained mainly from post-menopausal urine, but also from human pituitary glands, human chorionic gonadotrophin (HCG) extracted from the urine of pregnant women, and clomiphene (Clomid - Merrell), a synthetic compound which we reviewed in 1967.1

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