INHIBITION BY NORMAL IMMUNOGLOBULINS OF THYROTROPHIN-STIMULATED PRODUCTION OF CYCLIC AMP IN SLICES OF NORMAL HUMAN THYROID

1980 ◽  
Vol 87 (2) ◽  
pp. 271-277 ◽  
Author(s):  
S. P. BIDEY ◽  
N. J. MARSHALL ◽  
R. P. EKINS
Keyword(s):  
Cyclic Amp ◽  
Immunoglobulin G ◽  
Thyroid Tissue ◽  
Human Thyroid ◽  
Human Thyroid Tissue ◽  
Thyroid Stimulating Antibodies ◽  
Cyclic Amp Accumulation ◽  
Normal Human ◽  
Stimulation Of

Slice preparations of normal human thyroid tissue have been used to investigate the effect of normal immunoglobulin G (IgG) on thyrotrophin (TSH)-induced accumulation of cyclic AMP. Incubation of slices in the presence of both TSH and normal IgG for 20 min reduced the stimulation of cyclic AMP accumulation elicited by TSH alone by approximately 30%. However, preincubation of slices with IgG for 100 min before addition of TSH virtually abolished the response to TSH. The latter effect of normal IgG was reversible, and removal of IgG before exposure to TSH allowed an unimpaired cyclic AMP response to TSH. The implications of these observations with respect to the application of this system to the functional bio-detection of thyroid-stimulating antibodies in IgG fractions from thyrotoxic sera are discussed.

Cyclic AMP release from normal human thyroid slices in response to thyrotrophin

1980 ◽  
Vol 95 (3) ◽  
pp. 335-340 ◽  
Author(s):  
Stephen P. Bidey ◽  
Nicholas J. Marshall ◽  
Roger P. Ekins
Keyword(s):  
Cyclic Amp ◽  
Incubation Time ◽  
Thyroid Tissue ◽  
Human Thyroid ◽  
Dose Dependency ◽  
Incubation Periods ◽  
In Vitro Exposure ◽  
Normal Human ◽  
Stimulation Of

Abstract. Slice preparations of normal human thyroid tissue were incubated in vitro with TSH. The cyclic AMP contents of slices were determined at intervals up to 120 min, and cyclic AMP in the incubation medium was also estimated for each incubation period. Slice cyclic AMP levels were related both to incubation time and TSH dose. In response to 10 mU TSH/ml, slice cyclic AMP levels were maximal within 60 min, and were not significantly changed at 120 min. Cyclic AMP was detectable in the medium within 10 min of slice exposure to TSH, and increased throughout the initial 60 min of incubation. Cyclic AMP release during this period was dependent on both TSH dose and incubation time. Between 60–120 min, however, cyclic AMP release partially lost its TSH dose-dependency, and 0.5–5.0 mU TSH/ml were equipotent with respect to the final medium cyclic AMP level attained. Slices incubated without TSH released only small amounts of cyclic AMP, and maximal levels were attained within 20 min. In contrast to the adenylate cyclase response of thyroid membrane preparations, which was stimulated by NaF, suggesting that cyclic AMP release was not a result of the stimulation of damaged cells. These findings demonstrate the importance of cyclic AMP release from human thyroid slices, following in vitro exposure to TSH, and suggest that, after incubation periods such as are used for the functional biodetection of thyroid stimulators, the magnitude of cyclic AMP release may be of quantitative significance.

Characterisation of the cyclic AMP response to thyrotrophin in monolayer cultures of normal human thyroid cells

1981 ◽  
Vol 98 (3) ◽  
pp. 370-376 ◽  
Author(s):  
Stephen P. Bidey ◽  
Nicholas J. Marshall ◽  
Roger P. Ekins
Keyword(s):  
Cyclic Amp ◽  
Dose Range ◽  
Thyroid Tissue ◽  
Human Thyroid ◽  
Thyroid Cells ◽  
Monolayer Cultures ◽  
Normal Human ◽  
Stimulation Of ◽  
In Cells

Abstract. The cyclic AMP response to thyrotrophin (TSH) has been investigated in cells prepared from human thyroid tissue obtained during surgery for sub-total laryngectomy, and maintained under in vitro conditions as primary monolayer cultures. When cells were incubated with 1.0 mU TSH/ml, a maximal level of intracellular cyclic AMP was reached after 20 min of incubation in the presence of 0.5 mm 3-isobutyl-1-methyl xanthine (MIX). This level of cyclic AMP was sustained for at least 2 h. Half-maximal stimulation of cyclic AMP was produced by TSH doses of between 1 and 5 mU/ml. In a study of a series of eight groups of monolayer cultures, each derived from a single, different thyroid gland, the mean stimulation of cyclic AMP given by 50 mU TSH/ml was 37.8-fold greater than in non-stimulated cell monolayers. Significant stimulation to 50 μU TSH/ml was observed in some monolayers and the precision of measurement of TSH was better than 15% over the TSH dose range 0.2–1.0 mU/ml. The magnitude of the cyclic AMP response to TSH was unaffected by the presence in the incubation medium of 20% (v/v) normal human serum. A cyclic AMP response to TSH was still demonstrable in cells that had been maintained for a period of 22 days in monolayer culture, although the response was reduced in comparison with that given by 4–5 day old cultures.

IN-VITRO STUDIES OF NORMAL HUMAN THYROID CELLS: RESPONSES TO THYROTROPHIN AND DIBUTYRYL CYCLIC AMP

1977 ◽  
Vol 72 (1) ◽  
pp. 87-96 ◽  
Author(s):  
S. P. BIDEY ◽  
P. MARSDEN ◽  
J. ANDERSON ◽  
C. G. McKERRON ◽  
H. BERRY
Keyword(s):  
Cyclic Amp ◽  
Thyroid Tissue ◽  
Three Dimensional ◽  
Human Thyroid ◽  
Dibutyryl Cyclic Amp ◽  
Iodide Uptake ◽  
Thyroid Cells ◽  
Normal Human

SUMMARY Follicular cells isolated from normal human thyroid tissue have been cultured for up to 140 h with bovine thyrotrophin (TSH) or dibutyryl cyclic AMP (DBcAMP). Both compounds induced marked reorganization of the cells into three-dimensional follicular structures, whilst non-supplemented cells assumed a monolayer form. Cultures treated initially with TSH or DBcAMP showed a greater iodide uptake capacity, in comparison with unsupplemented cultures, in which iodide uptake was markedly diminished after 24 h. The release of tri-iodothyronine (T3) and thyroxine (T4) into the medium was determined by radioimmunoassay. Both TSH- and DBcAMP-treated cells showed a significant increase in iodothyronine output compared with unsupplemented control cells. In contrast to the 'classical' TSH-induced depression of the T4:T3 ratio in vivo, an increase in the ratio was observed for both TSH- and DBcAMP-supplemented cells in vitro. The ratio was also significantly greater after TSH than after DBcAMP, and possible implications of this finding are discussed.

Regulation of Cyclic Nucleotide and Prostaglandin Formation in Normal Human Thyroid Tissue and in Autonomous Nodules*

1980 ◽  
Vol 50 (4) ◽  
pp. 776-785 ◽  
Author(s):  
J. VAN SANDE ◽  
J. MOCKEL ◽  
J. M. BOEYNAEMS ◽  
P. DOR ◽  
G. ANDRY ◽  
...  
Keyword(s):  
Cyclic Nucleotide ◽  
Thyroid Tissue ◽  
Human Thyroid ◽  
Human Thyroid Tissue ◽  
Normal Human

Guanyl nucleotide regulatory proteins in neoplastic and normal human thyroid tissue

1988 ◽  
Vol 12 (4) ◽  
pp. 538-544 ◽  
Author(s):  
O. H. Clark ◽  
E. T. Gum ◽  
A. E. Siperstein ◽  
P. L. Gerend
Keyword(s):  
Thyroid Tissue ◽  
Regulatory Proteins ◽  
Human Thyroid ◽  
Human Thyroid Tissue ◽  
Normal Human
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