scholarly journals Effect of long-term glucocorticoid treatment on oestradiol-induced proliferation in the uterus of ovariectomized rats

1998 ◽  
Vol 157 (3) ◽  
pp. 481-488 ◽  
Author(s):  
AG Gunin
Keyword(s):  
Proliferating Cell Nuclear Antigen ◽  
Term Treatment ◽  
Labelling Index ◽  
Ovariectomized Rats ◽  
Nuclear Antigen ◽  
Glucocorticoid Treatment ◽  
Initial Reduction ◽  
Long Term Treatment ◽  
Proliferating Cell

The aim of this study was to examine the effect of long-term treatment with glucocorticoids on the uterine response to oestradiol. Ovariectomized rats were treated with crystal triamcinolone acetonide (0.1 mg/100 g, i.m.) or saline (0.1 ml/100 g i.m.) for 29 days. Over this period five injections were administered, one per week. On the second day after the last triamcinolone injection, rats were treated with a single injection of oestradiol dipropionate (5 micrograms/100 g, s.c.) or vehicle (olive oil, 0.1 ml/100 g, s.c.). The effects of oestradiol in the uterus were determined by measuring mitotic index, bromodeoxyuridine (BrdU)-labelling index (BrdU was injected 2 h before the rats were killed; 2 mg/100 g, i.p.), and proliferating cell nuclear antigen (PCNA)-labelling index 24, 36 and 48 h after the injection of oestradiol or vehicle. Long-term treatment with glucocorticoids resulted in dissimilar changes in oestradiol-induced proliferation in epithelial and connective-tissue (stroma) components of the uterus. In luminal and glandular epithelia, there was an initial reduction in proliferation at 24 h, followed by an increase at 36 h and a further reduction at 48 h after the oestradiol injection. In stromal cells of the endometrium, triamcinolone treatment caused a large constant increase in oestradiol-induced proliferation throughout the experiment. The glucocorticoid treatment had no effect on the parameters without oestradiol administration.

scholarly journals Reducing the Toxicity of Long-Term Glucocorticoid Treatment in Large Vessel Vasculitis

2020 ◽  
Vol 22 (12) ◽  
Author(s):  
Andriko Palmowski ◽  
Frank Buttgereit
Keyword(s):  
Adverse Events ◽  
Takayasu Arteritis ◽  
Term Treatment ◽  
Large Vessel Vasculitis ◽  
Large Vessel ◽  
Serious Adverse Events ◽  
Glucocorticoid Treatment ◽  
Long Term Treatment ◽  
Higher Doses

Abstract Purpose While glucocorticoids (GCs) are effective in large vessel vasculitis (LVV), they may cause serious adverse events (AEs), especially if taken for longer durations and at higher doses. Unfortunately, patients suffering from LVV often need long-term treatment with GCs; therefore, toxicity needs to be expected and countered. Recent Findings GCs remain the mainstay of therapy for both giant cell arteritis and Takayasu arteritis. In order to minimize their toxicity, the following strategies should be considered: GC tapering, administration of conventional synthetic (e.g., methotrexate) or biologic (e.g., tocilizumab) GC-sparing agents, as well as monitoring, prophylaxis, and treatment of GC-related AEs. Several drugs are currently under investigation to expand the armamentarium for the treatment of LVV. Summary GC treatment in LVV is effective but associated with toxicity. Strategies to minimize this toxicity should be applied when treating patients suffering from LVV.

scholarly journals Effects of Long-Term Treatment with Estradiol and Estrogen Receptor Subtype Agonists on Serotonergic Function in Ovariectomized Rats

2015 ◽  
Vol 103 (3-4) ◽  
pp. 269-281 ◽  
Author(s):  
Saloua Benmansour ◽  
Opeyemi S. Adeniji ◽  
Anthony A. Privratsky ◽  
Alan Frazer
Keyword(s):  
Estrogen Receptor ◽  
Term Treatment ◽  
Ovariectomized Rats ◽  
Receptor Subtype ◽  
Long Term Treatment ◽  
Serotonergic Function

Effects of preventive long-term treatment with strontium ranelate and zoledronic acid to ovariectomized rats on bone microstructure

2014 ◽  
Author(s):  
Marta Martin-Fernandez ◽  
Marina Gomez-Chinchon ◽  
David Guede ◽  
Jose Ramon Caeiro ◽  
Manuel Diaz-Curiel ◽  
...  
Keyword(s):  
Zoledronic Acid ◽  
Strontium Ranelate ◽  
Bone Microstructure ◽  
Term Treatment ◽  
Ovariectomized Rats ◽  
Long Term Treatment

scholarly journals Long-Term Treatment of Indomethacin Reduces Vertebral Bone Mass and Strength in Ovariectomized Rats

1997 ◽  
Vol 12 (11) ◽  
pp. 1844-1850 ◽  
Author(s):  
Hitoshi Saino ◽  
Toshikatsu Matsuyama ◽  
Junichi Takada ◽  
Tohru Kaku ◽  
Seiichi Ishii
Keyword(s):  
Bone Mass ◽  
Term Treatment ◽  
Ovariectomized Rats ◽  
Vertebral Bone ◽  
Long Term Treatment ◽  
Vertebral Bone Mass

scholarly journals Expression of proliferating cell nuclear antigen in luminal epithelium during the growth and regression of rat uterus

2000 ◽  
Vol 166 (1) ◽  
pp. 87-93 ◽  
Author(s):  
MD Lai ◽  
LR Lee ◽  
KS Cheng ◽  
LY Wing
Keyword(s):  
Cell Proliferation ◽  
Proliferating Cell Nuclear Antigen ◽  
Uterine Epithelium ◽  
Ovariectomized Rats ◽  
Nuclear Antigen ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Luminal Epithelium ◽  
Proliferating Cells ◽  
Uterine Growth ◽  
Proliferating Cell

Proliferating cell nuclear antigen (PCNA), a processivity factor of DNA synthesis, has often been used as a marker that reveals proliferating cells. However, it also plays a role other than in DNA replication. The aim of this study was to examine the relationship between the expression of PCNA and cell proliferation, and also its relation to cell death in the uterine epithelium under various hormonal conditions. Rats with regular estrous cycles were killed at various stages of the cycle, and their uteri were removed for the detection of PCNA and apoptosis by immunohistochemical and terminal deoxynucleotidyl transferase-mediated nick end-label staining respectively. There was an inverse relationship between the expression of PCNA and apoptosis in the uterine epithelium during the estrous cycle. From diestrus to proestrus, the expression of PCNA increased, and few apoptotic cells were detected in the luminal epithelium. However, at estrus, apoptosis occurred markedly, and the expression of PCNA disappeared. To study further the effects of estrogen on PCNA expression and cell growth in the uterus, rats were ovariectomized and then implanted s.c. with estrogen capsules 2 weeks later. In ovariectomized rats, only a few PCNA-positive cells were observed in the uterine epithelium. After estrogen treatment, PCNA was expressed strongly in the luminal and glandular epithelia. In these rats, the removal of estrogen capsules resulted in apoptotic death and surprisingly strong PCNA expression in the cells of luminal epithelium. Our results demonstrate that PCNA is expressed not only in the estrogen-stimulated uterine growth, but also in the processes of regression induced by the withdrawal of estrogen. Although the expression of PCNA has been reported to represent cell proliferation, our results implicate functions other than cell replication for PCNA in the uterus.

Sign in / Sign up

Export Citation Format

Share Document