scholarly journals Uremic plasma contains factors inhibiting 1 alpha-hydroxylase activity.

1992 ◽  
Vol 3 (4) ◽  
pp. 947-952
Author(s):  
C H Hsu ◽  
S Patel
Keyword(s):  
Enzyme Activity ◽  
Kinetic Analysis ◽  
Normal Saline ◽  
Saline Solution ◽  
Uremic Toxin ◽  
Enzyme Kinetic ◽  
Hydroxylase Activity ◽  
Normal Plasma ◽  
Guanidinosuccinic Acid ◽  
Plasma Ultrafiltrate

The effect of uremic plasma ultrafiltrate on calcitriol synthesis was investigated. Renal 1 alpha-hydroxylase activity was measured in normal rats infused for 20 h with 20 mL of normal or uremic plasma ultrafiltrate. Renal 1 alpha-hydroxylase activity was determined by the generation of calcitriol measured 5, 10, 20, and 30 min after the reaction was initiated by the addition of cold 25(OH)D3. The activity was significantly lower in rats infused with uremic plasma ultrafiltrate. Kidney homogenates preincubated for 3 h with uremic plasma ultrafiltrate also had significantly lower renal 1 alpha-hydroxylase activity than did those preincubated with normal plasma ultrafiltrate. In addition, the effect of the putative uremic toxin, guanidinosuccinic acid (GSA), on renal 1 alpha-hydroxylase activity was studied. Normal rats infused for 20 h with 20 mL of saline solution containing 1.5 mg/dL of GSA had significantly lower renal 1 alpha-hydroxylase activity than did rats infused with normal saline. The enzyme activity was also lower in kidney homogenates preincubated for 3 h with 4 mg/dL of GSA. Enzyme kinetic analysis revealed that the inhibition of renal 1 alpha-hydroxylase by GSA was noncompetitive. It was concluded that uremic plasma contains substances that directly inhibit renal 1 alpha-hydroxylase activity.

Kinetic Aspects of the Interaction of Blood Clotting Enzymes

1965 ◽  
Vol 13 (01) ◽  
pp. 155-175 ◽  
Author(s):  
H. C Hemker ◽  
P.W Hemker ◽  
E. A Loeliger
Keyword(s):  
Kinetic Analysis ◽  
Enzyme Kinetics ◽  
Blood Clotting ◽  
Enzyme System ◽  
Enzyme Kinetic ◽  
Clotting Time ◽  
Standard Methods

SummaryApplication of the methods of enzyme-kinetic analysis to the results of clotting tests is feasible and can yield useful results. However, the standard methods of enzyme kinetics are not applicable without modifications imposed by the peculiarities of the blood-clotting enzyme system. The influence of the following complicating circumstances is calculated :1. Substrate is not present in excess.2. Only relative measures exist for concentrations of substrate or enzymes.3. Enzymes and substrates are often added together.4. Reagents are not pure.5. Clotting-time is our only measure for clotting-velocity.Formulas are deduced, which makes it possible to recognize the effect of these complications.

Alteplase: Stability and Bioactivity after Dilution in Normal Saline Solution

2003 ◽  
Vol 14 (1) ◽  
pp. 99-102 ◽  
Author(s):  
Charles P. Semba ◽  
Suzanne Weck ◽  
Thomas Patapoff
Keyword(s):  
Normal Saline ◽  
Saline Solution

scholarly journals CASE OF TRANSFUSION OF THE NORMAL SALINE SOLUTION.

The Lancet ◽  
1889 ◽  
Vol 133 (3420) ◽  
pp. 527 ◽  
Author(s):  
WalterHenry Brown
Keyword(s):  
Normal Saline ◽  
Saline Solution

Are Lactated Ringer’s Solution and Normal Saline Solution Equal with Regard to Coagulation?: Retracted

2002 ◽  
Vol 94 (2) ◽  
pp. 378-384 ◽  
Author(s):  
Joachim Boldt ◽  
G. Haisch ◽  
S. Suttner ◽  
B. Kumle ◽  
F. Schellhase
Keyword(s):  
Normal Saline ◽  
Saline Solution ◽  
Ringer’S Solution ◽  
Lactated Ringer's Solution

scholarly journals Comparative results of gastric submucosal injection with hydroxypropyl methylcellulose, carboxymethylcellulose and normal saline solution in a porcine model

2010 ◽  
Vol 47 (2) ◽  
pp. 184-187 ◽  
Author(s):  
Luciano Lenz ◽  
Veruska Di Sena ◽  
Frank S. Nakao ◽  
Gustavo Paulo de Andrade ◽  
Maria Rachel da Silveira Rohr ◽  
...  
Keyword(s):  
Median Time ◽  
Normal Saline ◽  
Thermal Injury ◽  
Porcine Model ◽  
Saline Solution ◽  
Hydroxypropyl Methylcellulose ◽  
Three Solutions ◽  
Submucosal Injection ◽  
Significant Difference ◽  
Comparative Results

CONTEXT: Endoscopic mucosal resection is an established modality for excision of sessile lesions in the gastrointestinal tract. Submucosal fluid injection creates a cushion and may prevent thermal injury and perforation. OBJECTIVES: This blind study investigated the performance of three different solutions to create submucosal fluid cushions in porcine stomach. METHODS: Three solutions were injected in the stomach of nine pigs BR1: normal saline solution, carboxymethylcellulose 0.5% and hydroxypropyl methylcellulose 0.25%. In each pig, submucosal injections with 6 mL per test-solution were performed. One drop of methylene blue was added to all injections for better visualization. The time for the bleb to disappear was recorded. RESULTS: The overall median time of visible submucosal cushion was 37 minutes (range 12-60 min) for hydroxypropyl methylcellulose, 31 minutes for carboxymethylcellulose (range 10-43 min) and 19 minutes for normal saline solution (range 8-37 min). There was no statistically significant difference neither between normal saline solution and carboxymethylcellulose (P = 0.146) nor carboxymethylcellulose and hydroxypropyl methylcellulose (P = 0.119) but the median duration of hydroxypropyl methylcellulose was significantly longer than normal saline solution (P = 0.039). CONCLUSIONS: The length of hydroxypropyl methylcellulose submucosal fluid cushion is longer in comparison with normal saline solution. The median time for carboxymethylcellulose was not longer than normal saline solution. Hydroxypropyl methylcellulose, in the concentration of 0.25%, may be a durable alternative for submucosal injection.

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