scholarly journals Optimization of in vitro solid culture of Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH hybrid strain

2021 ◽  
Vol 45 (4) ◽  
pp. 441-449
Author(s):  
Tufan Can ULU ◽  
Alper SUSURLUK
Keyword(s):  
Heterorhabditis Bacteriophora ◽  
Solid Culture ◽  
Hybrid Strain

Induction of Neoplasia in vitro in Hamster Kidney Tissue by Adenovirus 7-SV40 "Hybrid" Strain (LLE46)

1966 ◽  
Vol 121 (2) ◽  
pp. 486-489 ◽  
Author(s):  
A. S. Rabson ◽  
R. A. Malmgren ◽  
R. L. Kirschstein
Keyword(s):  
Kidney Tissue ◽  
Hybrid Strain

scholarly journals Temporary immersion biorreators: efficient technique for the propagation of the ‘Pircinque’ strawberry

2019 ◽  
Vol 41 (1) ◽  
Author(s):  
Samila Silva Camargo ◽  
Leo Rufato ◽  
Maicon Magro ◽  
André Luiz Kulkamp de Souza
Keyword(s):  
Liquid Medium ◽  
Culture Medium ◽  
Culture Media ◽  
Time Factors ◽  
Efficient Technique ◽  
Control Treatment ◽  
Solid Culture ◽  
Temporary Immersion ◽  
Solid Culture Medium

Abstract The in vitro propagation technique via temporary immersion bioreactors is a tool that, through the culture in a liquid medium, allows an increase in the efficiency of seedling production. Several researches with the strawberry crop have shown greater efficiency of the system compared to the conventional process of micropropagation in solid medium. In this sense, the objective herein was to establish a protocol of multiplication and rooting of the ‘Pircinque’ strawberry, in temporary immersion bioreactors. Two distinct and independent studies were carried out, characterized by the multiplication and rooting stages of strawberry explants, newly introduced and registered in Brazil. Two culture media (MS and KNOP) were studied and, as a control treatment, the growth of the explants in solid culture medium was evaluated with the addition of 5 g L-1 of agar. Different immersion times of the culture medium were explored: five or eight times a day, for 15 minutes. The study was composed of the culture medium and immersion time factors, as well as the control (solid) treatment. It was verified that the use of temporary immersion bioreactors system is an efficient technique for the multiplication and rooting of explants of strawberry cv. Pircinque, when compared to the conventional method of micropropagation with the use of solid culture medium, making it possible to optimize the production of seedlings in biofactories. The MS liquid medium, in contact with explants of ‘Pircinque’ strawberry five times a day, increased the growth of the aerial part and the root system.

scholarly journals Static Liquid Culture of Doritaenopsis Seedlings

HortScience ◽  
2008 ◽  
Vol 43 (1) ◽  
pp. 206-210 ◽  
Author(s):  
Wei-Ting Tsai ◽  
Chien-Young Chu
Keyword(s):  
Liquid Medium ◽  
Liquid Culture ◽  
Petri Dish ◽  
Liquid Media ◽  
Solid Culture ◽  
Sealing Material ◽  
Phase Liquid ◽  
Sealing Materials ◽  
Growth Of Seedlings

Methods for static liquid culture are described to improve the growth of Doritaenopsis (commercially known as Phalaenopsis) seedlings in vitro. The results showed that seeds not only germinated, but also grew faster in liquid medium. No hyperhydric seedlings were observed in liquid culture when liquid level was accurately controlled by culture density, medium volume, and sealing materials. Although the germination percent was unaffected by medium phase (liquid or solid), sowing density, medium volume, or sealing material, the growth of seedlings decreased as density increased or medium volume decreased. Seeds of 1.5 mg mixed with 20 mL of liquid medium per 9-cm petri dish sealed with two layers of parafilm prompted optimal results. Shoot growth also was enhanced while 75-day-old seedlings were subcultured in liquid media with or without support. Seedling growth was enhanced by adding 20 mL liquid media to 36 seedlings without support after 45 days of culture. It was expected that by static liquid culture, the period from sowing to ex vitro would be 1.5 months shorter than the traditional solid culture.

Use of temporary immersion bioreactors and solid culture medium in the in vitro propagation of pear rootstocks

2021 ◽  
pp. 113-120
Author(s):  
A.L. Arruda ◽  
F.R. Nerbass ◽  
A.A. Kretzschmar ◽  
L. Rufato ◽  
A.J. Posser ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Culture Medium ◽  
Solid Culture ◽  
Temporary Immersion ◽  
Solid Culture Medium

Lipids of in Vivo and in Vitro Cultured Heterorhabditis bacteriophora

1999 ◽  
Vol 15 (2) ◽  
pp. 113-118 ◽  
Author(s):  
Moeen A Abu Hatab ◽  
Randy Gaugler
Keyword(s):  
Heterorhabditis Bacteriophora

Induction of Neoplasia in vitro in Hamster Thyroid Tissue by SV40 And Adenovirus 7-SV40 "Hybrid" (Strain LLE46)

1966 ◽  
Vol 123 (2) ◽  
pp. 507-510 ◽  
Author(s):  
S. A. Wells ◽  
S. K. Orme ◽  
A. S. Rabson
Keyword(s):  
Thyroid Tissue ◽  
Hybrid Strain

scholarly journals Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1010
Author(s):  
Eucario Mancilla-Álvarez ◽  
Juan Antonio Pérez-Sato ◽  
Rosalía Núñez-Pastrana ◽  
José L. Spinoso-Castillo ◽  
Jericó J. Bello-Bello
Keyword(s):  
Culture Medium ◽  
Solid Medium ◽  
Survival Rates ◽  
Shoot Multiplication ◽  
Control Treatment ◽  
Multiplication Rate ◽  
Solid Culture ◽  
Solid Culture Medium ◽  
Semi Solid

Taro is important for its nutritional content, medicinal use, and bioethanol production. The aim of the present study was to compare different semi-automated bioreactors (SABs) during in vitro multiplication of C. esculenta. The SABs used were temporary immersion bioreactors (TIBs), SETIS™ bioreactors and ebb-and-flow bioreactors; semi-solid culture medium was used as a control treatment. At 30 d of culture, different developmental variables, determination of chlorophyll, stomatal content, and survival percentage during acclimatization were evaluated. SABs increased the shoot multiplication rate relative to the semi-solid medium; however, the SETIS™ bioreactor showed the highest shoot production, with 36 shoots per explant, and the highest chlorophyll content. The stomatal index was higher in the semi-solid medium compared to the SABs, while the percentage of closed stomata was higher in the SABs than in the semi-solid culture medium. The survival rate during acclimatization showed no differences among the culture systems assessed, obtaining survival rates higher than 99%. In conclusion, the SETIS™ bioreactor showed the highest multiplication rate; however, other bioreactor alternatives are available for semi-automation and cost reduction for micropropagation of C. esculenta.

scholarly journals Molecular genetics of the virulence plasmids of pathogenic Escherichia coli O104:H4

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Rachel Whelan ◽  
Gareth McVicker
Keyword(s):  
Virulence Plasmid ◽  
Hybrid Strain ◽  
E Coli ◽  
Virulence Plasmids ◽  
Plasmid Loss ◽  
Pathogenic Escherichia Coli ◽  
Wide Range ◽  
Large Outbreak ◽  
Uremic Syndrome

In 2011 a large outbreak of enterohemorrhagic gastroenteritis and haemolytic uremic syndrome (HUS) throughout Europe resulted in almost 4,000 infections, 845 cases of HUS and 54 fatalities. This was due to a dangerous exchange of mobile genetic elements (MGE) resulting in a hybrid strain of E. coli O104:H4. This strain carried an unusual combination of EAEC- and STEC-associated virulence factors on a plasmid and phage respectively. In vitro the virulence plasmid has exhibited unusual stability under a wide range of environmental stresses, contrasting with rapid plasmid loss in the human gut. This project will characterise plasmid encoded maintenance systems responsible for its unique stability. Current investigations focus on toxin-antitoxin (TA) systems involved in postsegregationalkilling which contribute to plasmid maintenance therefore resulting in increased virulence. By inducing expression of putative TA genes cloned onto lab-made plasmid vectors, we have analysed their function in the cell. Once characterised we will further investigate the effects of various environmental conditions able to disrupt these TA systems, ultimately resulting in plasmid loss. This atypical strain displayed heightened pathogenicity and providedun foreseen treatment challenges. We aim to further our understanding of MGE carriage in O104:H4 as a model to predict and combat future outbreaks of hybrid pathovars.

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