scholarly journals Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1010
Author(s):  
Eucario Mancilla-Álvarez ◽  
Juan Antonio Pérez-Sato ◽  
Rosalía Núñez-Pastrana ◽  
José L. Spinoso-Castillo ◽  
Jericó J. Bello-Bello
Keyword(s):  
Culture Medium ◽  
Solid Medium ◽  
Survival Rates ◽  
Shoot Multiplication ◽  
Control Treatment ◽  
Multiplication Rate ◽  
Solid Culture ◽  
Solid Culture Medium ◽  
Semi Solid

Taro is important for its nutritional content, medicinal use, and bioethanol production. The aim of the present study was to compare different semi-automated bioreactors (SABs) during in vitro multiplication of C. esculenta. The SABs used were temporary immersion bioreactors (TIBs), SETIS™ bioreactors and ebb-and-flow bioreactors; semi-solid culture medium was used as a control treatment. At 30 d of culture, different developmental variables, determination of chlorophyll, stomatal content, and survival percentage during acclimatization were evaluated. SABs increased the shoot multiplication rate relative to the semi-solid medium; however, the SETIS™ bioreactor showed the highest shoot production, with 36 shoots per explant, and the highest chlorophyll content. The stomatal index was higher in the semi-solid medium compared to the SABs, while the percentage of closed stomata was higher in the SABs than in the semi-solid culture medium. The survival rate during acclimatization showed no differences among the culture systems assessed, obtaining survival rates higher than 99%. In conclusion, the SETIS™ bioreactor showed the highest multiplication rate; however, other bioreactor alternatives are available for semi-automation and cost reduction for micropropagation of C. esculenta.

scholarly journals Temporary immersion biorreators: efficient technique for the propagation of the ‘Pircinque’ strawberry

2019 ◽  
Vol 41 (1) ◽  
Author(s):  
Samila Silva Camargo ◽  
Leo Rufato ◽  
Maicon Magro ◽  
André Luiz Kulkamp de Souza
Keyword(s):  
Liquid Medium ◽  
Culture Medium ◽  
Culture Media ◽  
Time Factors ◽  
Efficient Technique ◽  
Control Treatment ◽  
Solid Culture ◽  
Temporary Immersion ◽  
Solid Culture Medium

Abstract The in vitro propagation technique via temporary immersion bioreactors is a tool that, through the culture in a liquid medium, allows an increase in the efficiency of seedling production. Several researches with the strawberry crop have shown greater efficiency of the system compared to the conventional process of micropropagation in solid medium. In this sense, the objective herein was to establish a protocol of multiplication and rooting of the ‘Pircinque’ strawberry, in temporary immersion bioreactors. Two distinct and independent studies were carried out, characterized by the multiplication and rooting stages of strawberry explants, newly introduced and registered in Brazil. Two culture media (MS and KNOP) were studied and, as a control treatment, the growth of the explants in solid culture medium was evaluated with the addition of 5 g L-1 of agar. Different immersion times of the culture medium were explored: five or eight times a day, for 15 minutes. The study was composed of the culture medium and immersion time factors, as well as the control (solid) treatment. It was verified that the use of temporary immersion bioreactors system is an efficient technique for the multiplication and rooting of explants of strawberry cv. Pircinque, when compared to the conventional method of micropropagation with the use of solid culture medium, making it possible to optimize the production of seedlings in biofactories. The MS liquid medium, in contact with explants of ‘Pircinque’ strawberry five times a day, increased the growth of the aerial part and the root system.

Use of temporary immersion bioreactors and solid culture medium in the in vitro propagation of pear rootstocks

2021 ◽  
pp. 113-120
Author(s):  
A.L. Arruda ◽  
F.R. Nerbass ◽  
A.A. Kretzschmar ◽  
L. Rufato ◽  
A.J. Posser ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Culture Medium ◽  
Solid Culture ◽  
Temporary Immersion ◽  
Solid Culture Medium

scholarly journals Elicitation of Phenylpropanoids in Maqui (Aristotelia chilensis [Mol.] Stuntz) Plants Micropropagated in Photomixotrophic Temporary Immersion Bioreactors (TIBs).

2021 ◽  
Author(s):  
Giulia E Trentini ◽  
Makarena Rojas ◽  
Daniela Gajardo ◽  
Débora Alburquenque ◽  
Evelyn Villagra ◽  
...  
Keyword(s):  
Culture Medium ◽  
Wild Plants ◽  
Control Treatment ◽  
Multiplication Rate ◽  
Fresh Weight ◽  
Temporary Immersion ◽  
Air Treatment ◽  
The Third ◽  
Aristotelia Chilensis

Abstract A biotechnological system for the production of plants biomass and phenylpropanoids of maqui was developed in photomixotrophic TIBs. The in vitro maqui multiplication was evaluated using combinations of TDZ and BAP in TIBs 1L capacity. Treatment of MS basal supplemented with TDZ 1 mg/l shows the best results for the variables fresh weight and multiplication rate. Photomixotrophic conditions were standardized in media with 3%, 2%, 1%, 0% sucrose at a light intensity of 100 µM m− 2s− 1. The treatments reduced in sucrose (1% and 2%) and air supplemented with 0.4 MPa CO2 do not differ significantly in biomass production (fresh weight per cluster of plants) compared to the control treatment with sucrose 3% and standard air. Treatment with ABA (1m/l) induced the production and accumulation of phenylpropanoids metabolites in maqui cultures bioreactors. Phenylpropanoids in in vitro biomass of maqui and culture medium from TIBs were determined in parallel with control samples from wild plants and mature fruits. After the third day of analysis, not significant differences in polyphenols and anthocyanin contents were verified between the treatments of maqui in TIBs + ABA and controls. The non-significant differences in the contents of polyphenols and anthocyanin were maintained until the 15 days of analysis. The antioxidant capacity comparing samples of maqui in bioreactors and wild plants showed no significant differences by the ORAC test from day 5 to day 15 of the study.

scholarly journals In vitro multiplication of Swietenia macrophylla King (Meliaceae) from juvenile shoots.

2007 ◽  
Vol 17 (2) ◽  
pp. 109 ◽  
Author(s):  
Eliane De Souza Schottz ◽  
Antonio Nascim Kalil Filho ◽  
Anderson Luiz Tracz ◽  
Henrique Koehler ◽  
Luciana L. F. Ribas ◽  
...  
Keyword(s):  
Culture Medium ◽  
Shoot Formation ◽  
Nodal Segments ◽  
Multiplication Rate ◽  
Maximum Point ◽  
Swietenia Macrophylla ◽  
Important Species ◽  
Solid Culture Medium ◽  
Hypsipyla Grandella

Big-leaf mahogany (Swietenia macrophylla King) is an important species for timber production that is considered the most valuable in the world. For this reason its exploitation is indiscriminate and leads this species to the risk of extinction. Moreover, mahogany is difficult to regenerate naturally and, when used in reforestation programs, plants are severely damaged by the shoot-borer (Hypsipyla grandella Zellar). This work aimed at developing the multiplication stage of micropropagation of Swietenia macrophylla King using juvenile material. After desinfestation, seeds were germinated in MS solid culture medium. Shoot formation from seeds occurred during five months, giving 5.54 nodal segments per seed. These explants were excised, each containing one axillary bud, and transferred on multiplication media. Four experiments with cytokinins were conducted, using media supplemented with 6-benzylaminopurine (BAP) (2.5 to 50.0 μM), 2-isopentenyladenine (2-iP) (0; 1.1 to 8.8 μM), combinations of BAP (0; 2.5 to 50.0 μM) and 2-iP (2.2 μM). For the first treatments the basal culture medium was MS medium and in the last one MS and QL media were used in separate experiments. When BAP was tested alone, the maximum point of multiplication rate average was obtained on medium containing 23.61 µM, while 2-iP did not induce bud multiplication. On QL culture medium supplemented with the combinations of BAP (0; 2.5; 5.0; 10.0 e 20.0 μM ) and 2-iP (2.2 μM), there was no multiplication. The maximum point of multiplication rate average was 5.7 µM, obtained when the MS culture medium was supplemented with 18.51 µM BAP and 2.2 µM 2-iP.

scholarly journals Effects of Preharvest Methyl Jasmonate Elicitation and Electrical Stimulation on Camptothecin Production by In Vitro Plants of Ophiorrhiza ridleyana Craib

2021 ◽  
Vol 11 (10) ◽  
pp. 4555
Author(s):  
Supakit Pisitpaibool ◽  
Suchada Sukrong ◽  
Kijchai Kanjanaprapakul ◽  
Muenduen Phisalaphong
Keyword(s):  
Tissue Culture ◽  
Electrical Stimulation ◽  
Methyl Jasmonate ◽  
Culture Medium ◽  
Solid Culture ◽  
Direct Electric Current ◽  
Solid Culture Medium ◽  
The Mean ◽  
Stems And Leaves

To enhance plant camptothecin (CPT) production in vitro, 5-month-old Ophiorrhiza ridleyana Craib plant cultures were treated with solutions of methyl jasmonate (MeJA) dissolved in ethanol, which were applied to the surface of the solid culture medium. It was demonstrated that the maximum CPT content in the tissue-cultured plants was achieved after 12 h elicitation with 50 µM MeJA. The mean CPT contents in roots and stems were 50.8 and 67.0 µg/g DW, respectively, which were approximately 1.8- and 2.6-fold higher, respectively, than those of the control. However, MeJA elicitation showed no significant effect on CPT accumulation in O. ridleyana leaves. Moreover, it was found that direct electric current (DC) stimulation also significantly increased CPT accumulation in O. ridleyana. The treatment with DC at 20 mA for 3 min of stimulation enhanced 3-fold the CPT content in roots, stems, and leaves to 41.9, 36.0 and 19.6 µg/g DW, respectively, which were approximately 1.5-, 1.7- and 1.4-fold higher, respectively, as compared to those of the control. The results demonstrate that preharvest treatment by MeJA elicitation and electrical stimulation can be beneficial for secondary metabolite production of CPT in tissue-culture plants of O. ridleyana.

scholarly journals Investigation of the spore production of Paecilomyces spp. isolated from several agricultural soils with the biocontrol activily against Spodoptera litura

2018 ◽  
Vol 1 (T5) ◽  
pp. 58-68
Author(s):  
Linh Quoc Nguyen ◽  
Nhut Nhu Nguyen
Keyword(s):  
Spodoptera Litura ◽  
Solid Medium ◽  
Agricultural Soils ◽  
Management Strategies ◽  
Solid Culture ◽  
Biocontrol Activity ◽  
Semi Solid ◽  
Main Components ◽  
Native Strains

Paecilomyces is a fungus that parasites on various insect species. However, Paecilomyces has not been widely studied and applied in Vietnam. In this study, Paecilomyces spp. were isolated from several agricultural soils and identified based on the morphology and 28S rDNA gene sequencing. Biocontrol activities of Paecilomyces were measured in vitro against Spodoptera litura. The Paecilomyces strains with high biocontrol were studied for the spore acquisition on semi-solid culture. There were five isolated strains belonged to Paecilomyces (strain F01 belonged to P. javanicus, strain F02 belonged to Paecilomyces sp., strain F03 belonged to P. lilacinum and strains F04 and F05 belonged to P. lilacinus) from 33 different samples. In particular, both of F03 and F04 performed high biocontrol activity against S. litura after 10 days of inoculation. Optimization of spores production medium showed that F03 and F04 grew well on a defined semi-solid medium whose the main components were unpolished rice, wheat bran and husk of 55% humidity. The results indicated that the native strains of Paecilomyces were potential for applications to produce bioproducts for pest management strategies.

scholarly journals An Improved liquid Culture System for Efficient Shoot Multiplication in Aerva lanata (L.) Juss. Ex Schult.

2021 ◽  
Vol 31 (1) ◽  
pp. 35-42
Author(s):  
Kandhan Varutharaju ◽  
Chandrasekaran Thilip ◽  
Palusamy Raja ◽  
Ganesan Thiagu ◽  
Abubakker Aslam ◽  
...  
Keyword(s):  
Liquid Medium ◽  
Solid Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiplication Rate ◽  
Mass Propagation ◽  
Liquid Culture System ◽  
Aerva Lanata ◽  
Better Than

An improved in vitro mass propagation protocol was developed for Aerva lanata using MS liquid medium. The influence of MS medium (solid and liquid) with cytokinin (TDZ and BAP, respectively) were studied for shoot proliferation and growth. The liquid medium perfomed better than solid medium in shoot multiplication. The maximum shoot multiplication rate was (29.37 ± 0.64 shoots per explant), obtained in MS liquid medium which is containing 0.6 mg/l TDZ, 0.3 mg/l NAA and 0.2 mg/l IBA. Different volumes of liquid medium have been used, 30 ml of medium flask showed the maximum number of shoots. Liquid medium is better suited for in vitro propagation of A. lanata since the enhanced multiplication rate was observed with shorter subculture intervals. Plant Tissue Cult. & Biotech. 31(1): 35-42, 2021 (June)

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