scholarly journals A simple HPLC-UV Method for Therapeutic Drug Monitoring of Linezolid in human Plasma in low-resourced settings

2021 ◽  
Vol 7 (4) ◽  
pp. e21008-e21008
Author(s):  
Vijayakumar A ◽  
Sudha V ◽  
Alffenaar JW ◽  
Jeyakumar SM ◽  
Hemanth Kumar AK
Keyword(s):  
Human Plasma ◽  
High Performance ◽  
Routine Care ◽  
Therapeutic Drug ◽  
Chromatography Method ◽  
Resistant Tuberculosis ◽  
Multi Drug Resistant Tuberculosis ◽  
Relative Standard ◽  
Liquid Chromatography Method

OBJECTIVE: A high-performance liquid chromatography method for the estimation of Linezolid in human plasma was developed and validated. METHODS: Samples (100µµL) were deproteinized with acetonitrile and analyzed using LiChrospher 100, RP18e column with PDA detection at 254 nm. The flow rate of the isocratic mobile phase comprising of 0.1% formic acid in 1000 ml of water and acetonitrile in the ratio of 60:40 (v/v) was set at 1.0 ml/min. RESULTS: The calibration curve ranged from 0.50 to 20.0 µg/ml and was linear. The recovery ranged from 96% to 101%. The accuracy ranged from 98 to 101% and intra- and inter-day relative standard deviation was <4.58%. The method reliably eliminated interfering materials from plasma and R2 was 0.9973. The method described was applied to the determination of plasma LZD concentration in multi-drug-resistant tuberculosis patients who are treated with a dose of 600 mg LZD once daily. CONCLUSIONS: The developed method is suitable for determination of plasma LZD in routine care and considered feasible in less-resourced settings

scholarly journals A SIMPLE AND RAPID LIQUID CHROMATOGRAPHY METHOD FOR DETERMINATION OF LEVOFLOXACIN IN PLASMA

2017 ◽  
Vol 14 (1) ◽  
pp. 27-32 ◽  
Author(s):  
A. K. Hemanth Kumar ◽  
V. Sudha ◽  
G Ramachandran
Keyword(s):  
High Performance ◽  
Reversed Phase ◽  
Excitation Wavelength ◽  
Pharmacokinetic Studies ◽  
Chromatography Method ◽  
Resistant Tuberculosis ◽  
Multi Drug Resistant Tuberculosis ◽  
Relative Standard ◽  
Liquid Chromatography Method

Introduction: Levofloxacin (LFX) is one of the second line anti-tuberculosis drugs used in the treatment of multi drug resistant tuberculosis. Monitoring of LFX concentrations in plasma may be valuable to study its pharmacokinetics and drug-drug interaction when co-administered with other anti-tuberculosis drugs. We developed a high performance liquid chromatic method of determination of LFX in plasma.Methodology: The method involved deproteinisation of the sample with perchloric acid and analysis of the supernatant using a reversed-phase C18 column (150mm) and fluorescence detection at an excitation wavelength of 290 nm and an emission wavelength of 460 nm.Results: The assay was specific for LFX and linear from 0.25 to 10.0μg/ml. The relative standard deviation of intra- and inter-day assays was lower than 10%. The average recovery of LFX from plasma was 99%.Conclusion: A sensitive, specific and validated method for quantitative determination of LFX in plasma was developed .Due to its simplicity; the assay can be used for pharmacokinetic studies of LFX.SAARC J TUBER LUNG DIS HIV/AIDS, 2017; XIV(1), page: 27-32

scholarly journals Simple and Rapid Liquid Chromatography Method for Determination of Rifabutin in Plasma

2013 ◽  
Vol 9 (2) ◽  
pp. 26-29 ◽  
Author(s):  
AK Hemanth Kumar ◽  
V Sudha ◽  
Geetha Ramachandran
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Pharmacokinetic Studies ◽  
Chromatography Method ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatography Method ◽  
Liquid Chromatographic

A high performance liquid chromatographic method for determination of rifabutin in human plasma was  developed. The method involved deproteinisation of the sample with acetonitrile and analysis of the  supernatant using a reversed-phase C18 column (250mm) and UV detection at a wavelength of 265nm.  The assay was specific for rifabutin and linear from 0.025 to 10.0μg/ml. The relative standard deviation  of intra- and inter-day assays was lower than 10%. The method was able to remove interfering materials  in plasma, yielding an average recovery of rifabutin from plasma of 101%. Due to its simplicity, the assay  can be used for pharmacokinetic studies of rifabutin. SAARC Journal of Tuberculosis, Lung Diseases & HIV/AIDS; 2012; IX(2) 26-29 DOI: http://dx.doi.org/10.3126/saarctb.v9i2.7975

Rapid Determination of Formaldehyde in Dried Bean Milk Cream by HPLC with Pre-Column Derivatization

2012 ◽  
Vol 554-556 ◽  
pp. 1493-1497
Author(s):  
Qi Tong ◽  
Jian Zheng Song ◽  
Qiu Rong Li
Keyword(s):  
High Performance ◽  
Rapid Determination ◽  
Rapid Test ◽  
Chromatography Method ◽  
System A ◽  
Controlling System ◽  
Relative Standard ◽  
Liquid Chromatography Method ◽  
Set Up

A high-performance liquid chromatography method was set up for rapid determination of formaldehyde in dried bean milk cream. This thesis studies the Nash derivatization, derivative solubility, reaction time, amounts of Nash and derivative stability. The derivative is chromatographic separated by Agilent Zorbax Eclipse XDB-C18 column (4.6mm× 250mm, 5μm) and detected by index detector with VWD (412nm). The heater does not need the temperature-controlling system. A mobile phase was composed of acetonitrile and water (50:50, V/V) at a flow rate of 0.8 mL/min. Under the conditions of the above-mentioned test, the developed calibration curves displayed good linearity over a concentration range of 0.00 to 0.80mg/L, with a correlation coefficient exceeding 0.9998. Average spike recovery was found in a range of 88% to 91%, with a relative standard deviation (RSD) between 1.1% and 4.0%.The minimum detection limit of source is 0.013mg/Kg. The method can be used for rapid test on formaldehyde preservative in dried bean milk cream.

Sign in / Sign up

Export Citation Format

Share Document