scholarly journals Molecular studies of Potato mop-top virus (PMTV) in transgenic N. benthamiana and S. tuberosum

2002 ◽  
Vol 38 (SI 1 - 6th Conf EFPP 2002) ◽  
pp. 183-185
Author(s):  
A. Germundsson ◽  
M. Sandgren ◽  
H. Barker ◽  
E.I. Savenkov ◽  
J.P.T. Valkonen
Keyword(s):  
Nicotiana Benthamiana ◽  
Transgenic Potato ◽  
Infested Soil ◽  
Systemic Movement ◽  
Potato Plants ◽  
Cp Gene ◽  
Molecular Studies ◽  
Infested Field ◽  
Transgenic Potato Plants ◽  
Potato Mop Top Virus

Resistance to Potato mop-top virus (PMTV) was studied in Nicotiana benthamiana and potato (Solanum tuberosum cv.<br />Saturna) transformed with the coat protein (CP) gene of PMTV. In N. benthamiana plants mechanically inoculated with<br />PMTV, RNA2 was detected in leaves and roots in several plants in the absence of RNA3. When N. benthamiana was<br />grown in infested soil, viral RNA was detected in roots, but no systemic movement of PMTV to above-ground parts was<br />observed. The incidence of PMTV infections was reduced in the CP-transgenic potato plants grown in an infested field<br />in Sweden. However, in infected tubers, all three virus RNAs were detected.

scholarly journals Initial infection of roots and leaves reveals different resistance phenotypes associated with coat protein gene-mediated resistance to Potato mop-top virus

2002 ◽  
Vol 83 (5) ◽  
pp. 1201-1209 ◽  
Author(s):  
Anna Germundsson ◽  
Maria Sandgren ◽  
Hugh Barker ◽  
Eugene I. Savenkov ◽  
Jari P. T. Valkonen
Keyword(s):  
Coat Protein ◽  
Nicotiana Benthamiana ◽  
Protein Gene ◽  
Resistance Mechanism ◽  
Spongospora Subterranea ◽  
Mechanical Inoculation ◽  
Systemic Movement ◽  
Cp Gene ◽  
Transgenic Potatoes ◽  
Potato Mop Top Virus

Resistance to the pomovirus Potato mop-top virus (PMTV) was studied in potato (Solanum tuberosum cv. Saturna) and Nicotiana benthamiana transformed with the coat protein (CP) gene of PMTV. The incidence of PMTV infections was reduced in tubers of the CP-transgenic potatoes grown in the field in soil infested with the viruliferous vector, Spongospora subterranea. However, in those tubers that were infected, all three virus RNAs were detected and virus titres were high. The CP-transgenic N. benthamiana plants were inoculated with PMTV using two methods. Following mechanical inoculation of leaves, no RNA 3 (the CP-encoding RNA homologous to the transgene) was detected in leaves, but in some plants low amounts of RNA 3 were detected in roots; RNA 2 was readily detected in leaves and roots of several plants. Inoculation of roots using viruliferous S. subterranea resulted in infection of roots in all plants and the three PMTV RNAs were detected. However, no systemic movement of PMTV from roots to the above-ground parts was observed, indicating a novel expression of resistance. These data indicate that the CP gene-mediated resistance to PMTV specifically restricts accumulation of PMTV RNA 3, and is more effective in leaves than roots. Furthermore, expression of resistance is different depending on whether leaves or roots are inoculated. Data do not exclude the possibility that both a protein-mediated and an RNA-mediated resistance mechanism are involved.

Sucrose synthase activity does not restrict glycolysis in roots of transgenic potato plants under hypoxic conditions

Planta ◽  
1999 ◽  
Vol 210 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Sophia Biemelt ◽  
Mohammad Reza Hajirezaei ◽  
Michael Melzer ◽  
Gerd Albrecht ◽  
Uwe Sonnewald
Keyword(s):  
Sucrose Synthase ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Hypoxic Conditions ◽  
Transgenic Potato Plants

Transgenic potato plants expressing the nptII-gus marker genes affect survival and development of the Colorado potato beetle

Plant Science ◽  
2002 ◽  
Vol 162 (3) ◽  
pp. 373-380 ◽  
Author(s):  
Sébastien De Turck ◽  
Philippe Giordanengo ◽  
Anas Cherqui ◽  
Corinne Ducrocq-Assaf ◽  
Brigitte S Sangwan-Norreel
Keyword(s):  
Colorado Potato Beetle ◽  
Transgenic Potato ◽  
Marker Genes ◽  
Potato Plants ◽  
Potato Beetle ◽  
Survival And Development ◽  
Transgenic Potato Plants

Thermodynamic and structural properties of tuber starches from transgenic potato plants grown in vitro and in vivo

2015 ◽  
Vol 125 ◽  
pp. 214-223 ◽  
Author(s):  
Luybov A. Wasserman ◽  
Andrey I. Sergeev ◽  
Viktor G. Vasil’ev ◽  
Irina G. Plashchina ◽  
Nina P. Aksenova ◽  
...  
Keyword(s):  
Structural Properties ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants

Enhancement of metabolizing herbicides in young tubers of transgenic potato plants with the rat CYP1A1 gene

2002 ◽  
Vol 105 (4) ◽  
pp. 515-520 ◽  
Author(s):  
T. Yamada ◽  
T. Ishige ◽  
N. Shiota ◽  
H. Inui ◽  
H. Ohkawa ◽  
...  
Keyword(s):  
Transgenic Potato ◽  
Potato Plants ◽  
Cyp1a1 Gene ◽  
Transgenic Potato Plants
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