scholarly journals Six single nucleotide polymorphisms in adipocyte fatty acid-binding protein (A-FABP) gene in Beijing ducks

2010 ◽  
Vol 55 (No. 9) ◽  
pp. 398-400 ◽  
Author(s):  
N. Zhao ◽  
S.S. Hou ◽  
X.L. Liu ◽  
X.G. Yang ◽  
W. Huang
Keyword(s):  
Linkage Disequilibrium ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Protein A ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Exon 4 ◽  
Fabp Gene

PCR-SSCP was applied to analyze the polymorphisms of A-FABP gene in 4 lines of Beijing ducks (n = 400). The results showed that six SNPs were found in intron 3. There were no polymorphisms found in exon 3 or exon 4. The discovered SNPs were deposited in GenBank (Acc. No.: EU306611 and EU306610). The frequencies of haplotypes A/B in the Z4, Z2, Cherry Valley, Z4 × Z2 populations were 0.745/0.255, 0.764/0.236, 0.552/0.448, 0.672/0.328, respectively. The linkage disequilibrium was stated. The above described SNPs of A-FABP gene allow the incoming association analysis.

scholarly journals Up-regulation of the expression of the gene for liver fatty acid-binding protein by long-chain fatty acids

1996 ◽  
Vol 319 (2) ◽  
pp. 483-487 ◽  
Author(s):  
Claire MEUNIER-DURMORT ◽  
Hélène POIRIER ◽  
Isabelle NIOT ◽  
Claude FOREST ◽  
Philippe BESNARD
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Fold Increase ◽  
Long Chain Fatty Acids ◽  
Liver Fatty Acid ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Fabp Gene ◽  
Long Chain

The role of fatty acids in the expression of the gene for liver fatty acid-binding protein (L-FABP) was investigated in the well-differentiated FAO rat hepatoma cell line. Cells were maintained in serum-free medium containing 40 µM BSA/320 µM oleate. Western blot analysis showed that oleate triggered an approx. 4-fold increase in the cytosolic L-FABP level in 16 h. Oleate specifically stimulated L-FABP mRNA in time-dependent and dose-dependent manners with a maximum 7-fold increase at 16 h in FAO cells. Preincubation of FAO cells with cycloheximide prevented the oleate-mediated induction of L-FABP mRNA, showing that protein synthesis was required for the action of fatty acids. Run-on transcription assays demonstrated that the control of L-FABP gene expression by oleate was, at least in part, transcriptional. Palmitic acid, oleic acid, linoleic acid, linolenic acid and arachidonic acid were similarly potent whereas octanoic acid was inefficient. This regulation was also found in normal hepatocytes. Therefore long-chain fatty acids are strong inducers of L-FABP gene expression. FAO cells constitute a useful tool for studying the underlying mechanism of fatty acid action.

scholarly journals Association of the heart fatty acid-binding protein gene with quality of carcass and meat traits in pigs

2008 ◽  
Vol 60 (2) ◽  
pp. 408-413 ◽  
Author(s):  
F.C. Figueiredo ◽  
P.S. Lopes ◽  
A.P.G. Pinto ◽  
D.A.F. Paiva ◽  
P.T. Mendonça ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Large White ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Genetic Groups ◽  
Pcr Products ◽  
Fabp Gene

The heart fatty acid-binding protein (HFABP) gene was sequenced in parental animals of a F2 crossing of boars of the Brazilian native Piau breed with commercial sows (Landrace x Large White Pietrain). Primers used for PCR were designed to amplify four exons of the gene. The PCR products were sequenced and compared with the GenBank sequences. Differences between the generated sequences and the GenBank sequences were observed for both genetic groups. A total of 246 F2 animals were genotyped using the Hinf I restriction enzyme. Two genotypes were identified, 198 being animals HH and 48 Hh. The Hinf I SNP was significantly associated with weights of loin (bone-in) (P<0.05), jowl (P<0.05), sirloin (P<0.10), and kidneys (P<0.01). These results showed the potential of the H-FABP gene in marker-assisted selection programs for carcass traits in pigs.

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