scholarly journals Molecular S-genotyping of sweet cherry (Prunus avium L.) genetic resources

2019 ◽  
Vol 46 (No. 3) ◽  
pp. 146-152
Author(s):  
Josef Patzak ◽  
Alena Henychová ◽  
František Paprštein ◽  
Jiří Sedlák
Keyword(s):  
Genetic Resources ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Self Incompatibility ◽  
S Locus ◽  
Specific Pcr ◽  
Sweet Cherries ◽  
Allele Specific ◽  
S Allele ◽  
Specific Pcr Primer

Sweet cherries are self-incompatible, which is determined by a gametophytic self-incompatibility system (GSI). The self-incompatibility is controlled by a multi-allelic S-locus. Knowledge about the S-allele constitution of the cultivars is essential for fruit growers and breeders. Recently, molecular PCR-based methods have been developed to distinguish all S-alleles in sweet cherries. In our work, we analysed S-locus genotypes by 13 universal and allele-specific PCR primer combinations within 117 registered, old and local sweet cherry cultivars from the Czech genetic resources of the Research and Breeding Institute of Pomology in Holovousy, the Czech Republic. We confirmed the previous S-genotyping for 66 accessions except for Drogans Gelbe, Hedelfinger, Erika, Meckenheimer Frühe, Badeborner, Bing, Alfa, Gamma, Huldra, Rivan, Valerij Tschkalov, Viola and Winkler’s Frühe. It could be due to either mislabelling or mistakes in the previous analyses. Newly, S-genotyping was determined for 51 accessions in which we found 4 new S-loci combinations. We detected the S-locus combinations in 19 incompatibility groups. The most frequent incompatibility groups were III (S<sub>3</sub>S<sub>4</sub>), II (S<sub>1</sub>S<sub>3</sub>), IV (S<sub>2</sub>S<sub>3</sub>), and VI (S<sub>3</sub>S<sub>6</sub>) with 22, 20, 12 and 12 genotypes, respectively.  

scholarly journals Wild and Rare Self-Incompatibility Allele S17 Found in 24 Sweet Cherry (Prunus avium L.) Cultivars

2021 ◽  
Author(s):  
Agnes Kivistik ◽  
Liina Jakobson ◽  
Kersti Kahu ◽  
Kristiina Laanemets
Keyword(s):  
Sweet Cherry ◽  
Prunus Avium ◽  
Eastern European ◽  
S Locus ◽  
Sweet Cherries ◽  
S Alleles ◽  
Allele Specific ◽  
S Allele ◽  
Sweet Cherry Cultivars ◽  
Consensus Primers

AbstractThe pollination of self-incompatible diploid sweet cherry is determined by the S-locus alleles. We resolved the S-alleles of 50 sweet cherry cultivars grown in Estonia and determined their incompatibility groups, which were previously unknown for most of the tested cultivars. We used consensus primers SI-19/20, SI-31/32, PaConsI, and PaConsII followed by allele-specific primers and sequencing to identify sweet cherry S-genotypes. Surprisingly, 48% (24/50) of the tested cultivars, including 17 Estonian cultivars, carry the rare S-allele S17, which had initially been described in wild sweet cherries in Belgium and Germany. The S17-allele in Estonian cultivars could originate from ‘Leningradskaya tchernaya’ (S6|S17), which has been extensively used in Estonian sweet cherry breeding. Four studied cultivars carrying S17 are partly self-compatible, whereas the other 20 cultivars with S17 have not been reported to be self-compatible. The recommended pollinator of seven self-incompatible sweet cherries is of the same S-genotype, including four with S17-allele, suggesting heritable reduced effectiveness of self-infertility. We classified the newly genotyped sweet cherry cultivars into 15 known incompatibility groups, and we proposed four new incompatibility groups, 64–67, for S-locus genotypes S3|S17, S4|S17, S5|S17, and S6|S17, respectively, which makes them excellent pollinators all across Europe. Alternatively, the frequency of S17 might be underestimated in Eastern European populations and some currently unidentified sweet cherry S-alleles might potentially be S17.

scholarly journals Allele-specific PCR detection of sweet cherry self-incompatibility alleles S3, S4 and S9 using consensus and allele-specific primers in the Czech Republic

2014 ◽  
Vol 41 (No. 4) ◽  
pp. 153-159 ◽  
Author(s):  
K. Sharma ◽  
P. Sedlák ◽  
D. Zeka ◽  
P. Vejl ◽  
J. Soukup
Keyword(s):  
Sweet Cherry ◽  
Prunus Avium ◽  
Management Strategies ◽  
Self Incompatibility ◽  
Specific Primers ◽  
Specific Pcr ◽  
Allele Specific ◽  
Considerable Length ◽  
S Allele ◽  
Consensus Primers

&nbsp; Prunus avium species of the Rosaceae family exhibit gametophytic self-incompatibility. Determination of the self-incompatibility genotype of individuals is essential for genetic studies and the development of informed management strategies. The PCR-based detection of S-allele helps to promote and speed up traditional breeding activity and hence molecular analysis of the perspective genotypes has become more intensive in all cherry growing countries. The alleles S<sub>3</sub>, S<sub>4</sub> and S<sub>9</sub> from 34 accessions of Czech collections were determined using the polymerase chain reaction (PCR) method. Initially, DNA extracts were amplified with consensus primers that amplify across the first, second, or both introns of the S-ribonuclease gene which shows a considerable length polymorphism. The new allele specific primers were designed with the goal to overcome some occurring difficulties in the detection of expected alleles by previously published allele specific primers. S-alleles fragments of standard cultivars used in this study were PCR amplified, sequenced to validate the designed primers. The study demonstrates the advantage of newly designed primers application in testing of sweet cherry genotypes. &nbsp; &nbsp;

scholarly journals Identification of Self-incompatibility Genotypes in Apple and Crabapple Cultivars by S-allele Specific PCR Analysis

2014 ◽  
Vol 46 (4) ◽  
pp. 364-371
Author(s):  
Kang Hee Cho ◽  
Jeong-Hee Kim2 ◽  
Jung Woo Lee ◽  
Soon-Il Kwon ◽  
Jong Taek Park ◽  
...  
Keyword(s):  
Pcr Analysis ◽  
Self Incompatibility ◽  
Specific Pcr ◽  
Allele Specific ◽  
S Allele ◽  
Allele Specific Pcr

scholarly journals Variable fruit set in self-fertile sweet cherry

2001 ◽  
Vol 81 (4) ◽  
pp. 753-760 ◽  
Author(s):  
Cheol Choi, and Robert L. Andersen
Keyword(s):  
Sweet Cherry ◽  
Prunus Avium ◽  
Fruit Set ◽  
Spring Frost ◽  
Self Pollination ◽  
Frost Injury ◽  
Sweet Cherries ◽  
Fruit Setting ◽  
S Allele ◽  
Sweet Cherry Cultivars

Fruit set on seven self-fertile (SF) sweet cherry genotypes from the Cornell Sweet Cherry Breeding Program was investigated. The S-alleles of all tested genotypes, based on controlled pollination and PCR-based S-allele typing analysis were determined to be: S1S4’ for Lapins, and S3S4’ for Stella, Starkrimson, NY 13688, NY 13696, NY 13788 and NY 13791. Higher fruit set of open-pollinated self-fertile genotypes indicated that the additional pollen donor was probably advantageous for fruit set in self-fertiles in the preliminary field study. However, no statistically significant differences were found between fully and self- (half compatible) pollination in caged trees in subsequent years’ studies. This inconsistency may have been due to shading by cages (for self-pollination only) or to differences in bee activity in caged trees as compared with open-pollinations made during preliminary studies. Additionally, the variation in fruit setting potential was dependent on genotype. Spring frost injury reduced fruit setting potential in self-fertile sweet cherries but pollen quality was not associated with variable fruit set. The results suggest that self-pollination would produce optimal fruit set in self-fertile sweet cherry cultivars regardless of partial pollen incompatibility. However, a more detailed study including fruit setting factors other than spring frost injury, and varying setting capacity depending on genotypes, would be required for a more complete determination of the commercial utility of growing self-fertile sweet cherries in solid blocks. Key words: Prunus avium L, sweet cherry, pollination, fruit set

scholarly journals Determination of (in)compatibility genotypes of Hungarian sweet cherry (Prunus avium L.) accessions by PCR based methods

2003 ◽  
Vol 9 (3-4) ◽  
Author(s):  
Zs. Békefi ◽  
K. R. Tobutt ◽  
T. Sonneveld
Keyword(s):  
Western Europe ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Traditional Test ◽  
Sweet Cherries ◽  
S Alleles ◽  
S Allele ◽  
Prunus Avium L

Sweet cherries (Prunus avium L.) are generally self-incompatible and pollinator cultivars are needed in orchards for reliable yield. In Hungary, choosing the appropriate cross-compatible cultivar pairs has so far been based on traditional test-crosses in the field. In recent years PCR-based methods that allow the identification of the S-alleles responsible for compatibility have been elaborated. We have determined the S-allele constitution of 24 cultivars and four selections important to Hungarian growers and breeders using PCR-based methods developed at Horticulture Research International, East Malling. The 28 accessions had various pairs of 9 alleles including one new allele, Sr. They could be assigned to 12 of the existing incompatibility groups or to a new group (S4S12) for which the designation 'Group XXVII' is proposed. The cultivars `Krupnoplodnaja' and 'Rita' had novel genotypes, S5S9 and S5Sx, respectively and can be placed into group 0 that holds universal pollen donors. The genotype of the cultivar ‘Hedelfingeni óriás' grown in Hungary was found to be S3S4 and therefore different from the cultivar `Hedelfingen' that is widespread in Western Europe.

scholarly journals Variability of Old Sweet Cherries Found in Slovak Regions and Their Preservation

2017 ◽  
Vol 71 (3) ◽  
pp. 184-189
Author(s):  
Michaela Benková ◽  
Iveta Čičová ◽  
Daniela Benedikova ◽  
Lubomir Mendel ◽  
Miroslav Glasa
Keyword(s):  
Sweet Cherry ◽  
Prunus Avium ◽  
Fruit Size ◽  
Morphological Characteristics ◽  
Quality Parameters ◽  
Fruit Weight ◽  
Sweet Cherries ◽  
Prunus Mahaleb ◽  
Breeding Programmes ◽  
Prunus Avium L

Abstract The work is focused on the evaluation of variability of morphological and pomological characteristics of several old sweet cherries (Prunus avium L.) that were found in different Slovak regions. The experimental work has been performed during two years, 2014 and 2015. The following characteristics according to the descriptor list of subgenus Cerasus were evaluated - period of flowering and ripening, morphological characteristics of the flowers, fruit size, fruit weight, and quality parameters. The results showed high variability of evaluated accessions. From the 13 surveyed localities, the most valuable accessions were found in the locality Hornį Streda - places Čachtice, Krakovany, Nitra, and Brdárka. During the collecting expeditions, 170 accessions of sweet cherry, with fruit of the different quality were found. The most interesting accessions were grafted onto rootstocks with different intensity of growth (Prunus avium L., Prunus mahaleb L., and ‘Gisela5’). Some of the selected cherry accessions can be used for commercial growing after tests, while some of them can be used only for collection of genetic resources and as potential genitors in breeding programmes.

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