Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants
2011 ◽
Vol 57
(No. 7)
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pp. 277-280
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Keyword(s):
In conifers and other plants with long reproductive cycles, transformed embryogenic tissues can serve as a convenient source of plant material for the testing of insecticidal or fungicidal transgene efficiency. In this report, transgenic embryogenic tissue was obtained after the transformation of somatic embryos of Norway spruce (Picea abies (L.) Karst.) by Agrobacterium tumefaciens with the gus-intron chimeric gene. The stable integration of transgenes was confirmed by PCR and Southern hybridization. The transformation was successful only in a suitable embryogenic cell line sensitive to Agrobacterium. Out of the nine embryogenic lines tested only one gave transgenic callus.
Keyword(s):
2017 ◽
Vol 59
(1)
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pp. 93-103
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2000 ◽
Vol 30
(12)
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pp. 1867-1876
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2009 ◽
Vol 55
(No. 4)
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pp. 156-161
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Keyword(s):