scholarly journals Transformation of HBsAg (hepatitis B surface antigen) gene into tomato mediated by Agrobacterium tumefaciens

2011 ◽  
Vol 47 (No. 2) ◽  
pp. 69-77 ◽  
Author(s):  
T. Li ◽  
J.K. Sun ◽  
Z.H. Lu ◽  
Q. Liu
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Marker Gene ◽  
Selection Marker ◽  
35S Promoter ◽  
Antigen Gene ◽  
Plant Expression ◽  
Plant Expression Vector

The plant expression vector pBRSAg was constructed as suitable for transformation via Agrobacterium-mediated approach. It contains all elements for plant expression, such as CaMV 35S promoter, both left and right border sequence for transferred DNA (T-DNA) in Agrobacterium, plant reporter gene gus, and plant selection marker gene hpt. The recombinant binary vector pBRSAg was transformed into Agrobacterium tumefaciens strains by using the freeze-thaw method. Tomato cotyledon explants were transformed by A. tumefaciens and plants were regenerated on selection medium. GUS staining, PCR and PCR-Southern analysis of the plants were positive. It was for the first time shown that the HBsAg (hepatitis B surface antigen) gene was introduced into tomato plants. The expression of transgene is under investigation.

Enhanced production of hepatitis B surface antigen in NIH 3T3 mouse fibroblasts by using extrachromosomally replicating bovine papillomavirus vector

1983 ◽  
Vol 3 (6) ◽  
pp. 1032-1039
Author(s):  
Y Wang ◽  
C Stratowa ◽  
M Schaefer-Ridder ◽  
J Doehmer ◽  
P H Hofschneider
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Bovine Papillomavirus ◽  
Mouse Fibroblasts ◽  
Antigen Gene ◽  
B Virus ◽  
Nih 3T3 ◽  
Papillomavirus Dna

We have constructed a recombinant pBR322 plasmid composed of a subgenomic transforming fragment of bovine papillomavirus DNA and the hepatitis B surface antigen gene from cloned hepatitis B virus DNA and used it for transfection of NIH 3T3 mouse fibroblasts. The transformed cells retain the plasmids in extrachromosomal form with a copy number of about 50 to 100 per cell. Expression of the hepatitis B surface antigen gene linked to bovine papillomavirus DNA is independent of its orientation relative to the bovine papillomavirus vector. Cell lines continuously secreting high amounts of hepatitis B surface antigen into the medium could be established. The antigen is released into the culture medium as 22-nm particles, having the same physical properties and constituent polypeptides as those found in the serum of hepatitis B virus-infected patients.

scholarly journals Hepatitis B surface antigen gene expression is regulated by sex steroids and glucocorticoids in transgenic mice.

1987 ◽  
Vol 84 (5) ◽  
pp. 1187-1191 ◽  
Author(s):  
H. Farza ◽  
A. M. Salmon ◽  
M. Hadchouel ◽  
J. L. Moreau ◽  
C. Babinet ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Sex Steroids ◽  
Hepatitis B Surface Antigen ◽  
Antigen Gene

scholarly journals Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis.

2013 ◽  
Vol 60 (3) ◽  
Author(s):  
Jindřich Bříza ◽  
Daniela Pavingerová ◽  
Josef Vlasák ◽  
Hana Niedermeierová
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Bacillus Thuringiensis ◽  
Picea Abies ◽  
Norway Spruce ◽  
Somatic Embryos ◽  
Marker Gene ◽  
Selection Marker ◽  
Embryogenic Tissue ◽  
35S Promoter ◽  
Rt Pcr

Modified versions of the Cry3A gene of Bacillus thuringiensis (Bt) were transferred into Norway spruce (Picea abies). Both the biolistic approach and Agrobacterium tumefaciens mediated procedure were employed for transformation of embryogenic tissue (ET) cultures. The latter method proved to be more efficient yielding 70 transgenic embryogenic tissue lines compared with 18 lines obtained by biolistics. The modified Cry3A genes were driven by a 35S promoter and the nptII screenable selection marker gene was used in all vectors. The transgenic ETs were molecularly characterized and converted into mature somatic embryos. Germinating embryos formed plantlets which were finally planted into perlite and their Cry3A gene transcription activities were demonstrated by RT-PCR.

scholarly journals Deep sequencing of hepatitis B surface antigen gene in the preserved umbilical cords in immunoprophylaxis failure against mother-to-child HBV transmission

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Haruki Komatsu ◽  
Ayano Inui ◽  
Yasuto Suzuki ◽  
Masaya Sugiyama ◽  
Tomoo Fujisawa
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Deep Sequencing ◽  
Hepatitis B Surface Antigen ◽  
Child Transmission ◽  
Antigen Gene ◽  
Born Child ◽  
A Minor ◽  
Mother To Child ◽  
Umbilical Cords

Abstract Background Vaccine escape mutants (VEMs) are one of the causes of breakthrough infections in the mother-to-child transmission of hepatitis B virus (HBV). We hypothesized that VEMs existing as minor populations in the maternal blood are associated with breakthrough infections in children. We sought to determine whether VEMs exist as minor populations in the preserved umbilical cords of children with breakthrough infections. Case presentation Two families (Family 1: three children, Family 2: two children) were enrolled. Despite immunoprophylaxis, a breakthrough infection occurred in two Family 1 children and two Family 2 children. Preserved umbilical cords, serum, and nails were used for the HBV DNA analysis. To detect VEMs, we performed direct and deep sequencing of hepatitis B surface antigen gene. The direct sequencing showed that there were no VEMs in the serum of the children or mother of Family 1 and family 2, but it identified a G145A mutant in the nails of the mother of Family 2. In Family 1, deep sequencing detected a T143S mutant as a minor population (1.7–2.0%) in the umbilical cords and serum of all three children and in the serum of the mother. A T126A mutant was also detected in the umbilical cord (9.2%) and serum (7.0%) of the first-born child of Family 1. In Family 2, the deep sequencing showed no VEMs in the umbilical cords, but it detected D144A (2.5%) and G145A (11.2%) mutants in the serum of the 2nd-born child. Conclusions VEMs were present as minor populations in the preserved umbilical cords of children with breakthrough infections. The VEMs did not become major populations after the breakthrough infections. The evolution of VEMs from a minor form to a major form might not be a prerequisite for breakthrough infections in mother-to-child transmission.

scholarly journals Enhanced production of hepatitis B surface antigen in NIH 3T3 mouse fibroblasts by using extrachromosomally replicating bovine papillomavirus vector.

1983 ◽  
Vol 3 (6) ◽  
pp. 1032-1039 ◽  
Author(s):  
Y Wang ◽  
C Stratowa ◽  
M Schaefer-Ridder ◽  
J Doehmer ◽  
P H Hofschneider
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Bovine Papillomavirus ◽  
Mouse Fibroblasts ◽  
Antigen Gene ◽  
B Virus ◽  
Nih 3T3 ◽  
Papillomavirus Dna

We have constructed a recombinant pBR322 plasmid composed of a subgenomic transforming fragment of bovine papillomavirus DNA and the hepatitis B surface antigen gene from cloned hepatitis B virus DNA and used it for transfection of NIH 3T3 mouse fibroblasts. The transformed cells retain the plasmids in extrachromosomal form with a copy number of about 50 to 100 per cell. Expression of the hepatitis B surface antigen gene linked to bovine papillomavirus DNA is independent of its orientation relative to the bovine papillomavirus vector. Cell lines continuously secreting high amounts of hepatitis B surface antigen into the medium could be established. The antigen is released into the culture medium as 22-nm particles, having the same physical properties and constituent polypeptides as those found in the serum of hepatitis B virus-infected patients.

scholarly journals Expression of hepatitis B surface antigen gene in yeast.

1983 ◽  
Vol 80 (1) ◽  
pp. 1-5 ◽  
Author(s):  
A. Miyanohara ◽  
A. Toh-e ◽  
C. Nozaki ◽  
F. Hamada ◽  
N. Ohtomo ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Antigen Gene
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