Molecular identification of wheat leaf rust resistance genes in sixty Chinese wheat cultivars

Common wheat (Triticum aestivum L.) is the major crop cultivated in Xinjiang and Anhui provinces of China. The climate in these two provinces is favourable for wheat leaf rust (Puccinia triticina) (Pt) infection. Here, we demonstrate a detailed investigation on the leaf rust resistance of 60 major wheat cultivars cultivated in these two regions. A mixture of high virulent Pt races (THTT, THTS, THTQ and PHPS) were used to phenotype all the collected wheat cultivars at an adult plant stage. Phenotypic disease severity (FDS) and the area under the disease progress curve (AUDPC) for each of these wheat cultivars were calculated. Among all the tested wheat cultivars, three cultivars (Xindong20, Xindong 29 and 99AR142-1) with the lowest FDS and AUDPC may carry major resistance genes. Twenty-seven cultivars (45% of the total tested ones) showed a relatively lower resistance with an average of 12.52% FDS and 126.3 AUDPC. Minor resistance or slow rusting genes may be present in this group of cultivars. Molecular markers for leaf rust resistance genes Lr1, Lr9, Lr19, Lr24, Lr26 and Lr34 were further used for the genotypic screening. Lr1, Lr19, Lr26 and Lr34 were detected in 19 (31.7%), 1 (1.7%), 12 (20%) and 6 (10%) wheat cultivars, respectively. Neither Lr9 nor Lr24 could be detected in any of the tested cultivars. These results will greatly improve wheat molecular breeding for leaf rust resistance in these areas.

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Analysis of Wheat Leaf Rust Resistance Genes in 14 Wheat Cultivars or Lines

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2011.02158 ◽

2012 ◽

Vol 37 (12) ◽

pp. 2158-2166 ◽

Cited By ~ 1

Author(s):

Ya-Ya HU ◽

Na ZHANG ◽

Lin-Mao LI ◽

Wen-Xiang YANG ◽

Da-Qun LIU

Keyword(s):

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Wheat Cultivars ◽

Wheat Leaf Rust ◽

Wheat Leaf ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

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Performance of Four New Leaf Rust Resistance Genes Transferred to Common Wheat from Aegilops tauschii and Triticum monococcum

Plant Disease ◽

10.1094/pdis.1997.81.6.582 ◽

1997 ◽

Vol 81 (6) ◽

pp. 582-586 ◽

Cited By ~ 18

Author(s):

T. Hussien ◽

R. L. Bowden ◽

B. S. Gill ◽

T. S. Cox ◽

D. S. Marshall

Keyword(s):

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Aegilops Tauschii ◽

Leaf Rust Resistance ◽

Wheat Leaf Rust ◽

Wheat Leaf ◽

Moderately Resistant ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

The objective of this study was to test the performance of four new wheat leaf rust resistance genes previously transferred from wild relatives of common wheat. Leaf rust resistance gene Lr43, in wheat germplasm line KS92WGRC16, was originally from Aegilops tauschii. A second resistance gene, in line KS92WGRC23, was transferred from Triticum monococcum var. monococcum. Two other genes, in lines KS93U3 and KS96WGRC34, were obtained from T. monococcum var. boeoticum. In greenhouse tests, the typical low infection types produced by these lines were fleck (;), immune (0), fleck with chlorosis (;C), and heterogeneous (X-) for KS92WGRC16, KS92WGRC23, KS96WGRC34, and KS93U3, respectively. In field tests in Kansas and Texas, KS92WGRC23 and KS92WGRC16 were highly resistant. KS93U3 was moderately resistant in Kansas but moderately resistant to moderately susceptible in Texas. KS96WGRC34 was moderately resistant in Kansas but moderately resistant to susceptible in Texas. Greenhouse adult-plant tests with race PBJL of Puccinia recondita f. sp. tritici indicated that KS92WGRC16, KS92WGRC23, and KS96WGRC34 were highly resistant, but KS93U3 gave a moderately resistant reaction. Growth-chamber studies in different environments (12, 16, 20, and 24°C) showed slight temperature effects on the expression of resistance in KS96WGRC34 but not in the other lines. Tests with nine races of P. recondita f. sp. tritici indicated that only KS92WGRC16 was resistant to all the races. Races PNML and PNMQ were virulent on KS92WGRC23, and race TFGL was virulent on both KS93U3 and KS96WGRC34. The genes in the four germplasm lines should be used in combination with other resistance genes to prolong their usefulness.

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Evaluation of Wheat Leaf Rust Resistance Genes in 10 Wheat Genotypes

Journal of Plant Diseases and Protection ◽

10.1007/bf03356536 ◽

2015 ◽

Vol 122 (2) ◽

pp. 91-99

Author(s):

Xuejun Wei ◽

Heshan Zhang ◽

Dongdong Du ◽

Wenxiang Yang ◽

Daqun Liu

Keyword(s):

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Wheat Leaf Rust ◽

Wheat Genotypes ◽

Wheat Leaf ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

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Alien genetic resources for wheat leaf rust resistance, cytogenetic transfer, and molecular analysis

Australian Journal of Agricultural Research ◽

10.1071/ar07315 ◽

2008 ◽

Vol 59 (3) ◽

pp. 197 ◽

Cited By ~ 24

Author(s):

B. S. Gill ◽

L. Huang ◽

V. Kuraparthy ◽

W. J. Raupp ◽

D. L. Wilson ◽

...

Keyword(s):

Leaf Rust ◽

Common Wheat ◽

Resistance Genes ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Wheat Leaf Rust ◽

D Genome ◽

Wheat Leaf ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

Wild relatives of wheat are useful sources of alien resistance genes for wheat breeding. The objective of this review is to document research on the evaluation, transfer, and molecular analysis of alien resistance to wheat leaf rust especially in Aegilops tauschii, the diploid D-genome donor of common wheat. Nine named resistance genes (Lr1, Lr2, Lr15, Lr21, Lr22, Lr32, Lr34, Lr39, and Lr42) occur in the D genome. Twelve new leaf rust resistance genes have been documented in Ae. tauschii. The south-west Caspian Sea region is the centre of genetic diversity for seedling resistance. Adult-plant resistance is widespread in all geographic regions and should be exploited more in the future. Lr1 and Lr21 have been cloned and are typical NBS-LRR genes. The recent documentation of cryptic introgressions of Lr57/Yr40 from Ae. geniculata and Lr58 from Ae. triuncialis offers exciting possibilities for transferring alien genes without linkage drag. Both Lr21 and Lr34 presumably arose during or following the origin of common wheat ~8000 years ago. Leaf rust resistance genes often are located towards the physical ends of wheat chromosomes. These regions are known to be high in recombination, and this may explain their rapid rate of evolution.

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Race and Virulence Dynamics of Puccinia triticina in China During 2000–2006

Plant Disease ◽

10.1094/pdis-06-10-0460-re ◽

2012 ◽

Vol 96 (11) ◽

pp. 1601-1607 ◽

Cited By ~ 11

Author(s):

T. G. Liu ◽

W. Q. Chen

Keyword(s):

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Puccinia Triticina ◽

Leaf Rust Resistance ◽

Code System ◽

Wheat Leaf Rust ◽

Wheat Leaf ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

Wheat leaf rust, caused by Puccinia triticina, is an important foliar disease of wheat in China. The dynamics of races and virulence in P. triticina populations in China during 2000 to 2006 were studied. Leaf rust samples were collected during surveys of wheat fields and trap nurseries in 16 provinces, and provided by coworkers throughout China. The virulence of single-pustule isolates was determined on near-isogenic Thatcher lines for leaf rust resistance genes Lr1, Lr2a, Lr2c, Lr3, Lr9, Lr16, Lr24, Lr26, Lr3ka, Lr11, Lr17, and Lr30, and races were denominated using the Prt code system. During 2000 to 2006, 79 races were identified from a total of 613 isolates. Races PHT (23.7%), THT (14.7%), PHJ (11.4%), and THJ (4.2%) were the four common races, all avirulent to Lr9 and Lr24. The frequency of isolates with virulence to Lr1, Lr2c, Lr3, Lr11, Lr16, Lr17, and Lr26 was over 80%, and these isolates were widely distributed in China, whereas the frequencies of virulence to Lr9, Lr19, Lr24, Lr25, Lr28, and Lr29 were 0.2 to 2.5%. The diversity of virulence phenotypes of Chinese P. triticina populations appeared to increase from 2000 to 2006. P. triticina races and virulences in China appear to be isolated from those in other countries.

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Virulence of wheat leaf rust in Slovakia in 1997–1998

Plant Protection Science ◽

10.17221/9704-pps ◽

1999 ◽

Vol 35 (No. 3) ◽

pp. 85-92

Author(s):

P. Bartoš ◽

J. Huszár ◽

E. Herzová

Keyword(s):

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Field Conditions ◽

Wheat Leaf Rust ◽

Wheat Leaf ◽

First Time ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

In 1997–1998 virulence of the leaf rust population was studied on near isogenic Thatcher lines with the genes for resistance Lr1, Lr2a, Lr2b, Lr2c, Lr3, Lr9, Lrll, Lr15, Lr/7, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28, and on the standard differentials Mala koff, Carina, Brevit, Webster, Loros, Mediterranean, Hussar, Democrat and the supplemental cultivar Salzmtinder Bartweizen. All 55 analyzed rust samples were avirulent on Lr9, Lr19, Lr24 and Lr28.On the standard differentials, races 61SaBa, 77SaBa, 77/57SaBa, 2SaBa, 77, 12SaBa, 62SaBa, 6, 6SaBa and 14 were determined. Races 61SaBa and 77SaBa (77/57SaBa) prevailed in both years. Races 6 and 6SaBa were found for the first time. The effectiveness of leaf rust resistance genes in registered cultivars under field conditions in variety trials is discussed.

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Identification of Leaf Rust Resistance Genes in Selected Wheat Cultivars and Development of Multiplex PCR

Open Life Sciences ◽

10.1515/biol-2019-0036 ◽

2019 ◽

Vol 14 (1) ◽

pp. 327-334 ◽

Cited By ~ 2

Author(s):

Agnieszka Tomkowiak ◽

Roksana Skowrońska ◽

Alicja Buda ◽

Danuta Kurasiak-Popowska ◽

Jerzy Nawracała ◽

...

Keyword(s):

Multiplex Pcr ◽

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Field Resistance ◽

Wheat Cultivars ◽

Pcr Conditions ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

AbstractTen leading wheat cultivars originating from the Plant Breeding and Acclimatization Institute (IHAR) - National Research Institute (Poland) and the Department of Gene Bank (Czech Republic) were used to establish a field experiment in 2017 and 2018 at the Dłoń Experimental Farm. The analyzed wheat genotypes were characterized by diversified field resistance to leaf rust. Jubilatka, Thatcher and Sparta were the most resistant cultivars in field conditions in both 2017 and 2018. The aim of the work was to identify the Lr11, L13, Lr16 and Lr26 genes encoding resistance to leaf rust using molecular SSR markers (wmc24, wmc261, Xgwm630, Xwmc764 and P6M12) and to develop multiplex PCR conditions to accelerate identification of these genes. Markers of three leaf rust resistance genes have been identified simultaneously in these cultivars. Jubilatka, Thatcher and Sparta cultivars may serve as a good source of the analyzed leaf rust resistance genes. In addition, multiplex PCR conditions have been developed for the simultaneous identification of the Lr11 and Lr16 and Lr11 and Lr26 gene pairs.

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