scholarly journals Wheat leaf rust (Puccinia triticina Eriks.) virulence frequency and detection of resistance genes in wheat cultivars registered in the Czech Republic in 2016–2018

2020 ◽  
Vol 56 (No. 3) ◽  
pp. 87-92 ◽  
Author(s):  
Alena Hanzalová ◽  
Veronika Dumalasová ◽  
Ondřej Zelba
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Puccinia Triticina ◽  
Near Isogenic Lines ◽  
Wheat Cultivars ◽  
Wheat Leaf Rust ◽  
The Czech Republic ◽  
Lr Genes ◽  
Wheat Leaf ◽  
Virulence Frequency

In 2016–2018 virulence of the Czech wheat leaf rust population was studied on Thatcher near-isogenic lines, carrying different Lr genes, and 130 leaf rust isolates. Virulence to Lr9 was found only sporadically. Virulence frequency to Lr2a, Lr2b, Lr2c and Lr28 was lower than in previous years. All tested isolates were avirulent to Lr19. Lr24 conditioned resistance to majority of isolates. Nineteen recently registered Czech cultivars were tested with six isolates of the pathogen and Lr genes were postulated. Presence of genes Lr1, Lr10, Lr19, Lr24, Lr26, Lr28, Lr34 and Lr37 was tested by molecular markers. Lr37 prevailed, followed by Lr genes 10, 24, 28, 1 and 26; genes Lr19 and Lr34 were not determined.

scholarly journals Physiologic specialization of wheat leaf rust (Puccinia triticina Eriks.) in the Slovak Republic in 2005, 2006 and 2008

2010 ◽  
Vol 46 (No. 3) ◽  
pp. 114-121 ◽  
Author(s):  
A. Hanzalová ◽  
J. Huszár ◽  
E. Herzová ◽  
P. Bartoš
Keyword(s):  
Leaf Rust ◽  
Slovak Republic ◽  
Puccinia Triticina ◽  
Near Isogenic Lines ◽  
Wheat Cultivars ◽  
Wheat Leaf Rust ◽  
Wheat Leaf ◽  
Winter Wheat Cultivars ◽  
Low Incidence ◽  
Different Parts

In 2005, 2006 and 2008 the virulence of wheat leaf rust population was studied on Thatcher near-isogenic lines with Lr1, Lr2a, Lr2b, Lr2c, Lr3a, Lr9, Lr10, Lr13, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28. Samples of leaf rust (141 in total) were obtained from different parts of Slovakia. Resistance gene Lr9 was effective to all tested isolates except three isolates from 2008. No virulence was found to Lr19 and genes Lr24 and Lr28 were also highly effective. Low incidence of virulence to Lr2a was observed. Sixty-five winter wheat cultivars registered in Slovakia were tested with seven leaf rust isolates in the greenhouse. Cultivar Bona Dea was the most resistant of all.

scholarly journals Geographical Distribution of Physiologic Races of Puccinia triticina and Postulation of Resistance Genes in New Wheat Cultivars in Egypt

2012 ◽  
Vol 1 (1) ◽  
pp. 73-80 ◽  
Author(s):  
Nour E. K. Soliman ◽  
Ashraf M. M. Abdelbacki ◽  
Mohammad A.A. Najeeb ◽  
Reda I. Omara
Keyword(s):  
Leaf Rust ◽  
Geographical Distribution ◽  
Resistance Genes ◽  
Puccinia Triticina ◽  
Wheat Cultivars ◽  
Wheat Leaf Rust ◽  
Rust Disease ◽  
Lr Genes ◽  
Wheat Leaf ◽  
Physiologic Races

Knowledge of the geographical distribution for physiologic races of Puccinia triticina and identification of leaf rust resistance genes (Lr ,s) in the recent Egyptian wheat cultivars are essential for maximizing resistance in future-bred cultivars. The  aim   of   this   study   was   to   know   the   status  of resistance in Egyptian wheat cultivars against wheat leaf rust and the most frequent race distributed. Infected samples were collected from five Governorates, i.e.,  Dakahlia,  Kafr el-Sheikh,  Beheira,  Sharqia  and  Sohag comprised the wheat growing area in Egypt. These samples were isolated, purified and identified on the differential stes. Gene postulation was done using fifteen identified races on Egyptian wheat cultivars correlated with Lr genes. Thirty three races identified during three seasons 2009/2010, 2010/2011 and 2011/2012. The most frequent race was TK (10%) followed by race BB (7.58%), PK (6.55%), TT (4.82%), PT (3.79%) and MT (3.44%). Moreover, races; BB, TT and PT were present during three seasons while these races appeared in some Governorates and disappeared in other Governorates. On the other hand, the most frequently occurring gene in ten Egyptian wheat cultivars was Lr35 (70%), followed by Lr22 (60%), Lr27 (40%), Lr34 (30%), Lr19 (30%),Lr18 (10%),Lr36 (10%) and Lr46 (10%), eight out of sixteen Lr genes were not present in the tested cultivars. It is concluded that there was a good variation in Lr genes carried by wheat cultivars commercially grown in Egypt. Therefore, strategies for deploying resistance genes to prolong effective disease resistance are suggested to control wheat leaf rust disease.

scholarly journals Wheat leaf rust races/pathotypes in the Czech Republic in 1999–2000

2001 ◽  
Vol 37 (No. 1) ◽  
pp. 10-16 ◽  
Author(s):  
P. Bartoš ◽  
A. Hanzalová ◽  
E. Stuchlíková
Keyword(s):  
Leaf Rust ◽  
Low Frequency ◽  
Near Isogenic Lines ◽  
Wheat Cultivars ◽  
Wheat Leaf Rust ◽  
Time Data ◽  
The Czech Republic ◽  
Wheat Leaf ◽  
Winter Wheat Cultivars ◽  
First Time

In 1999–2000 virulence of the wheat leaf rust population was studied on Thatcher near isogenic lines (NILs) with  Lr1, Lr2a, Lr2b, Lr2c, Lr3, Lr9, Lr10 (only in 2000), Lr11, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28. On these NILs 11 pathotypes were determined in 1999, and 18 in 2000. All rust samples were avirulent on Lr9, Lr19, Lr24 and Lr28. There was a relatively low frequency of virulence on Lr1, Lr2a and Lr2b. Most rust samples were virulent on Lr2c, Lr11, Lr15, Lr17, Lr21, Lr23 and Lr26. The identified pathotypes conformed with races 61SaBa, 77SaBa, 2SaBa, 12SaBa, 57SaBa, 6SaBa, 28SaBa, 61and 6. Races 61SaBa, 2SaBa and 77SaBa prevailed in 1999, races 61SaBa and 12SaBa were prevalent in 2000. Race 28SaBa was found for the first time. Data of the reactions to 15 leaf rust isolates of the recently registered winter wheat cultivars Niagara, Vlasta, Sulamit, Record, Ludwig, Apache, Semper, Drifter, Complet, Corsaire, Sepstra and Rialto are presented.

scholarly journals Pathotypes of wheat leaf rust (Puccinia triticina Eriks.) and resistance of registered cultivars in the Czech Republic in 2012–2015

2017 ◽  
Vol 53 (No. 3) ◽  
pp. 122-126 ◽  
Author(s):  
A. Hanzalová ◽  
P. Bartoš ◽  
T. Sumíková
Keyword(s):  
Czech Republic ◽  
Leaf Rust ◽  
Puccinia Triticina ◽  
Near Isogenic Lines ◽  
Wheat Leaf Rust ◽  
Chain Reaction ◽  
The Czech Republic ◽  
Wheat Leaf ◽  
Polymerase Chain ◽  
Different Parts

In 2012–2015 the virulence of the wheat leaf rust (Puccinia triticina Eriks.) population was studied on Thatcher near-isogenic lines with Lr1, Lr2a, Lr2b, Lr2c, Lr3a, Lr9, Lr10, Lr11, Lr13, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28. Samples of leaf rust were obtained from different parts of the Czech Republic. A total of 163 wheat leaf rust isolates were analysed. No virulence for the resistance gene Lr9 was found. Virulence for Lr19 was found only in one isolate in 2015. A lower frequency of virulence to Lr24, Lr2a, 2b, 2c and Lr28 was also observed. The presence of Lr10, Lr24, Lr26, Lr28 and Lr37 in registered cultivars was detected by polymerase chain reaction (PCR) molecular markers.

scholarly journals Wheat leaf rust races/pathotypes in the Czech Republic in 1997–1998

1999 ◽  
Vol 35 (No. 2) ◽  
pp. 51-56
Author(s):  
P. Bartoš ◽  
E. Stuchlíková
Keyword(s):  
Leaf Rust ◽  
Low Frequency ◽  
Near Isogenic Lines ◽  
Wheat Cultivars ◽  
Wheat Leaf Rust ◽  
Time Data ◽  
The Czech Republic ◽  
Wheat Leaf ◽  
Winter Wheat Cultivars ◽  
First Time

In 1997–1998 virulence of the wheat leaf rust population was studied on Thatcher near isogenic lines with Lr1, Lr2a, Lr2b, Lr2c, Lr3. Lr9, Lrll, Lrl5, Lrl7,  Lrl9,  Lr21, Lr23, Lr24, Lr26 and Lr28. On these NILs 11 pathotypes were determined in 1997, and 8 inl998. All rust samples were avirulent onLr9, Lr19, Lr24 and Lr28.There was a relatively low frequency of virulence on Lrl, Lr2a and Lr2b. Most rust samples were virulent on Lr2c, Lr11, Lr15, Lr17, Lr23 and Lr26. All samples were virulent on Lr3 and Lr21. The identified pathotypes conformed with races 61SaBa*, 77SaBa, 2SaBa, 12SaBa, 57SaBa, 6SaBa, 61*, 77 and 6. Races 61SaBa*, 77SaBa and 2SaBa prevailed in 1997, races 61 SaBa*, 77SaBa and 12SaBa were prevalent in 1998. Races 6 and 6SaBa were found for the first time. Data on Lr genes in recently registered winter wheat cultivars are presented.

scholarly journals Dissecting the first phased dikaryotic genomes of the wheat rust pathogen Puccinia triticina reveals the mechanisms of somatic exchange in nature

2019 ◽  
Author(s):  
Jing Qin Wu ◽  
Chongmei Dong ◽  
Long Song ◽  
Christina A. Cuomo ◽  
Robert F. Park
Keyword(s):  
Leaf Rust ◽  
Somatic Hybridization ◽  
Puccinia Triticina ◽  
Genomic Variation ◽  
Hybrid Wheat ◽  
Wheat Cultivars ◽  
Wheat Leaf Rust ◽  
Virulence Evolution ◽  
Rust Pathogen ◽  
Wheat Leaf

AbstractAlthough somatic hybridization (SH) has been proposed as a means of accelerating rust pathogen virulence evolution in the absence of sexual recombination, previous studies are limited to the laboratory and none have revealed how this process happens. Using long-read sequencing, we generated dikaryotic phased genomes and annotations for three Australian field-collected isolates of the wheat leaf rust pathogen (Puccinia triticina; Pt), including a putative asexual hybrid (Pt64) and two putative parental isolates (Pt104 and Pt53; 132-141 Mb,155-176 contigs, N50 of 1.9-2.1 Mb). The genetic dissection based on the high-quality phased genomes including whole-genome alignments, phylogenetic and syntenic analyses along with short-read sequencing of 27 additional Pt isolates convergently demonstrated that Pt64, which rendered several commercial hybrid wheat cultivars susceptible to leaf rust, arose from SH between isolates within the Pt53 and Pt104 lineages. Parentage analysis demonstrated the role of mitotic crossover in the derivation of both nuclei of Pt64. Within HD mating type genes, the distinct specificity regions in Pt64 and the distinct phylogenetic pattern of the remaining admixed isolates suggested high genetic variation in specificity-related regions on the b locus intrinsically associated with the SH. This study not only provided a fundamental platform for investigating genomic variation underlying virulence evolution in one of the most devastating wheat pathogens, but also offered an in-depth understanding of the mechanisms of naturally occurring SH. This asexual mechanism can be broadly exploited by any dikaryotic pathogen to accelerate virulence evolution, and understanding this process is both urgent and crucial for sustainable pathogen control.ImportanceStrategies to manage plant rust pathogens are challenged by the constant emergence of new virulence. Although somatic hybridization has been proposed as a means by which rusts could overcome host resistance rapidly and cause crop loss, there is very little evidence of this process in nature and the mechanisms underlying it are not known. This study generated and analysed the first dikaryotic phased genomes of the wheat leaf rust pathogen, identifying an isolate as a hybrid and for the first time unveiling parasexuality via mitotic crossover in a rust pathogen. The erosion of the resistance of several hybrid wheat cultivars in agriculture by the hybrid rust has important implications for breeding efforts targeting durable resistance and sustained rust control.

scholarly journals Physiologic Specialization of Wheat Leaf Rust (Puccinia triticina Eriks.) in theCzech Republic in 2001–2004

2011 ◽  
Vol 42 (No. 4) ◽  
pp. 126-131 ◽  
Author(s):  
A. Hanzalová ◽  
P. Bartoš
Keyword(s):  
Czech Republic ◽  
Leaf Rust ◽  
Puccinia Triticina ◽  
Field Trials ◽  
Wheat Leaf Rust ◽  
The Czech Republic ◽  
Wheat Leaf ◽  
Winter Wheat Cultivars ◽  
Physiologic Races ◽  
Different Parts

In 2001–2004 virulence of the wheat leaf rust population in the Czech Republic was studied on Thatcher near-isogenic lines with Lr1, Lr2a, Lr2b, Lr2c, Lr3a, Lr9, Lr10, Lr11, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28. Samples of leaf rust (180 in total) were obtained from different parts of the Czech Republic. Resistance genes Lr9 and Lr19 were effective to all tested isolates like in the previous years. Unlike the previous years virulence on Lr24 and Lr28 was observed. Relatively effective were also Lr1, Lr2a, Lr24, Lr28 and Lr2b. Other Lr genes were defeated by the majority of the tested samples. Our results transformed to the numbers of physiologic races indicate that race 61SaBa prevailed like in the previous years, followed by races 61, 2, 12SaBa, 2SaBa, 14, 77SaBa, 12, 57, 6, 53, 53SaBa, 77, and 14SaBa. Twenty-one winter wheat cultivars registered in 2001–2004 were tested with 8 leaf rust isolates. Out of them 15 showed resistance at least to one rust isolate. High resistance recorded in field trials for cvs Batis and Ilias, which were susceptible to all tested rust isolates at the seedling stage, demonstrates the importance of field (partial) resistance.  

scholarly journals Resistance of triticale to wheat leaf rust (Puccinia triticina)

2011 ◽  
Vol 47 (No. 1) ◽  
pp. 10-16 ◽  
Author(s):  
A. Hanzalová ◽  
P. Bartoš
Keyword(s):  
Czech Republic ◽  
Leaf Rust ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Near Isogenic Lines ◽  
The Czech Republic ◽  
Winter Triticale ◽  
Wheat Leaf ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Reactions of winter triticale cultivars mostly from central Europe to recent and old leaf rust isolates were tested in the greenhouse. In one trial 20 cultivars were tested with 8 leaf rust isolates, collected recently from official wheat and triticale trials in the Czech Republic and Slovakia. In another trial 15 cultivars were tested with 6 old leaf rust isolates, used to identify leaf rust resistance genes in wheat. The cultivars Cando, Hortenso and Tricolor, registered in the Czech Republic, were resistant to the majority of the recent isolates. The Hungarian cultivar Tatra was resistant to all the recent isolates. The old leaf rust isolates were virulent only to a few of the triticale cultivars. Randomly selected isolates from wheat and triticale were tested on triticale cultivars and on Thatcher near isogenic lines with different Lr genes. On average, leaf rust isolates from triticale were virulent to a higher number of triticale cultivars than isolates collected from wheat and vice versa.

scholarly journals Virulence surveys of wheat leaf rust in the Czech Republic and resistance genes in registered cultivars

2014 ◽  
Vol 50 (No. 3) ◽  
pp. 241-246 ◽  
Author(s):  
A. Hanzalová ◽  
P. Bartoš
Keyword(s):  
Czech Republic ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Wheat Cultivars ◽  
Wheat Leaf Rust ◽  
The Czech Republic ◽  
Technical Report ◽  
Wheat Leaf ◽  
Physiologic Races ◽  
The Given

The technical report presents a summary of results obtained in the leaf rust race surveys carried out in Czechoslovakia/Czech Republic in the years 1966–2001. The physiologic races were determined using the standard differentials Malakoff (Lr1), Carina (Lr2b), Brevit (Lr2c), Webster (Lr2a), Loros (Lr2c), Mediterranean (Lr3), Hussar (Lr11), Democrat (Lr3) and the additional differential Salzmünder Bartweizen (Lr26). Races 14, 77, 61, 53 and 2  successively dominated in the given period. They mostly appeared in two variants, avirulent and virulent to Lr26. Genes for virulence in the leaf rust races were compared with genes for resistance in the registered wheat cultivars listed in the report. Most frequent were the genes Lr3 and Lr26.

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