scholarly journals Virulence surveys of wheat leaf rust in the Czech Republic and resistance genes in registered cultivars

2014 ◽  
Vol 50 (No. 3) ◽  
pp. 241-246 ◽  
Author(s):  
A. Hanzalová ◽  
P. Bartoš
Keyword(s):  
Czech Republic ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Wheat Cultivars ◽  
Wheat Leaf Rust ◽  
The Czech Republic ◽  
Technical Report ◽  
Wheat Leaf ◽  
Physiologic Races ◽  
The Given

The technical report presents a summary of results obtained in the leaf rust race surveys carried out in Czechoslovakia/Czech Republic in the years 1966–2001. The physiologic races were determined using the standard differentials Malakoff (Lr1), Carina (Lr2b), Brevit (Lr2c), Webster (Lr2a), Loros (Lr2c), Mediterranean (Lr3), Hussar (Lr11), Democrat (Lr3) and the additional differential Salzmünder Bartweizen (Lr26). Races 14, 77, 61, 53 and 2  successively dominated in the given period. They mostly appeared in two variants, avirulent and virulent to Lr26. Genes for virulence in the leaf rust races were compared with genes for resistance in the registered wheat cultivars listed in the report. Most frequent were the genes Lr3 and Lr26.

scholarly journals Physiologic Specialization of Wheat Leaf Rust (Puccinia triticina Eriks.) in theCzech Republic in 2001–2004

2011 ◽  
Vol 42 (No. 4) ◽  
pp. 126-131 ◽  
Author(s):  
A. Hanzalová ◽  
P. Bartoš
Keyword(s):  
Czech Republic ◽  
Leaf Rust ◽  
Puccinia Triticina ◽  
Field Trials ◽  
Wheat Leaf Rust ◽  
The Czech Republic ◽  
Wheat Leaf ◽  
Winter Wheat Cultivars ◽  
Physiologic Races ◽  
Different Parts

In 2001–2004 virulence of the wheat leaf rust population in the Czech Republic was studied on Thatcher near-isogenic lines with Lr1, Lr2a, Lr2b, Lr2c, Lr3a, Lr9, Lr10, Lr11, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28. Samples of leaf rust (180 in total) were obtained from different parts of the Czech Republic. Resistance genes Lr9 and Lr19 were effective to all tested isolates like in the previous years. Unlike the previous years virulence on Lr24 and Lr28 was observed. Relatively effective were also Lr1, Lr2a, Lr24, Lr28 and Lr2b. Other Lr genes were defeated by the majority of the tested samples. Our results transformed to the numbers of physiologic races indicate that race 61SaBa prevailed like in the previous years, followed by races 61, 2, 12SaBa, 2SaBa, 14, 77SaBa, 12, 57, 6, 53, 53SaBa, 77, and 14SaBa. Twenty-one winter wheat cultivars registered in 2001–2004 were tested with 8 leaf rust isolates. Out of them 15 showed resistance at least to one rust isolate. High resistance recorded in field trials for cvs Batis and Ilias, which were susceptible to all tested rust isolates at the seedling stage, demonstrates the importance of field (partial) resistance.  

scholarly journals Geographical Distribution of Physiologic Races of Puccinia triticina and Postulation of Resistance Genes in New Wheat Cultivars in Egypt

2012 ◽  
Vol 1 (1) ◽  
pp. 73-80 ◽  
Author(s):  
Nour E. K. Soliman ◽  
Ashraf M. M. Abdelbacki ◽  
Mohammad A.A. Najeeb ◽  
Reda I. Omara
Keyword(s):  
Leaf Rust ◽  
Geographical Distribution ◽  
Resistance Genes ◽  
Puccinia Triticina ◽  
Wheat Cultivars ◽  
Wheat Leaf Rust ◽  
Rust Disease ◽  
Lr Genes ◽  
Wheat Leaf ◽  
Physiologic Races

Knowledge of the geographical distribution for physiologic races of Puccinia triticina and identification of leaf rust resistance genes (Lr ,s) in the recent Egyptian wheat cultivars are essential for maximizing resistance in future-bred cultivars. The  aim   of   this   study   was   to   know   the   status  of resistance in Egyptian wheat cultivars against wheat leaf rust and the most frequent race distributed. Infected samples were collected from five Governorates, i.e.,  Dakahlia,  Kafr el-Sheikh,  Beheira,  Sharqia  and  Sohag comprised the wheat growing area in Egypt. These samples were isolated, purified and identified on the differential stes. Gene postulation was done using fifteen identified races on Egyptian wheat cultivars correlated with Lr genes. Thirty three races identified during three seasons 2009/2010, 2010/2011 and 2011/2012. The most frequent race was TK (10%) followed by race BB (7.58%), PK (6.55%), TT (4.82%), PT (3.79%) and MT (3.44%). Moreover, races; BB, TT and PT were present during three seasons while these races appeared in some Governorates and disappeared in other Governorates. On the other hand, the most frequently occurring gene in ten Egyptian wheat cultivars was Lr35 (70%), followed by Lr22 (60%), Lr27 (40%), Lr34 (30%), Lr19 (30%),Lr18 (10%),Lr36 (10%) and Lr46 (10%), eight out of sixteen Lr genes were not present in the tested cultivars. It is concluded that there was a good variation in Lr genes carried by wheat cultivars commercially grown in Egypt. Therefore, strategies for deploying resistance genes to prolong effective disease resistance are suggested to control wheat leaf rust disease.

scholarly journals The virulence spectrum of the wheat leaf rust population analyzed in the Czech Republic from 2002 to 2011

2014 ◽  
Vol 50 (No. 4) ◽  
pp. 288-292
Author(s):  
A. Hanzalová ◽  
P. Bartoš
Keyword(s):  
Czech Republic ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Research Report ◽  
Wheat Leaf Rust ◽  
The Czech Republic ◽  
Wheat Leaf ◽  
Investigation Period ◽  
Infection Types

The research report presents a summary of wheat leaf rust virulence surveys in the Czech Republic from 2002 to 2011. Determination of virulence was based on infection types on Thatcher near-isogenic lines (NILs) with the resistance genes Lr1, Lr2a, Lr2b, Lr2c, Lr3a, Lr9, Lr10, Lr11, Lr13, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28, respectively. Virulence for Lr9 and Lr 19 occurred only sporadically in the investigated period. On average, virulence for Lr2a, Lr2b, Lr24 and Lr28 was low. The highest frequency of virulence was found for Lr3a, Lr10, Lr11, Lr13, Lr15, Lr17, Lr21, Lr23 and Lr26. During the investigation period we recorded a shift from prevailing virulence for Lr2c and avirulence for Lr1 to avirulence for aLr2c and virulence for Lr1. Ther virulences in the leaf rust population were compared with matching resistance genes  in the registered wheat cultivars. The most frequent resistance gene was Lr37, while the genes Lr3a and Lr26 were less frequent.

scholarly journals Wheat leaf rust races/pathotypes in the Czech Republic in 1999–2000

2001 ◽  
Vol 37 (No. 1) ◽  
pp. 10-16 ◽  
Author(s):  
P. Bartoš ◽  
A. Hanzalová ◽  
E. Stuchlíková
Keyword(s):  
Leaf Rust ◽  
Low Frequency ◽  
Near Isogenic Lines ◽  
Wheat Cultivars ◽  
Wheat Leaf Rust ◽  
Time Data ◽  
The Czech Republic ◽  
Wheat Leaf ◽  
Winter Wheat Cultivars ◽  
First Time

In 1999–2000 virulence of the wheat leaf rust population was studied on Thatcher near isogenic lines (NILs) with  Lr1, Lr2a, Lr2b, Lr2c, Lr3, Lr9, Lr10 (only in 2000), Lr11, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28. On these NILs 11 pathotypes were determined in 1999, and 18 in 2000. All rust samples were avirulent on Lr9, Lr19, Lr24 and Lr28. There was a relatively low frequency of virulence on Lr1, Lr2a and Lr2b. Most rust samples were virulent on Lr2c, Lr11, Lr15, Lr17, Lr21, Lr23 and Lr26. The identified pathotypes conformed with races 61SaBa, 77SaBa, 2SaBa, 12SaBa, 57SaBa, 6SaBa, 28SaBa, 61and 6. Races 61SaBa, 2SaBa and 77SaBa prevailed in 1999, races 61SaBa and 12SaBa were prevalent in 2000. Race 28SaBa was found for the first time. Data of the reactions to 15 leaf rust isolates of the recently registered winter wheat cultivars Niagara, Vlasta, Sulamit, Record, Ludwig, Apache, Semper, Drifter, Complet, Corsaire, Sepstra and Rialto are presented.

Analysis of Wheat Leaf Rust Resistance Genes in 14 Wheat Cultivars or Lines

2012 ◽  
Vol 37 (12) ◽  
pp. 2158-2166 ◽  
Author(s):  
Ya-Ya HU ◽  
Na ZHANG ◽  
Lin-Mao LI ◽  
Wen-Xiang YANG ◽  
Da-Qun LIU
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Wheat Cultivars ◽  
Wheat Leaf Rust ◽  
Wheat Leaf ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

scholarly journals Pathotypes of wheat leaf rust (Puccinia triticina Eriks.) and resistance of registered cultivars in the Czech Republic in 2012–2015

2017 ◽  
Vol 53 (No. 3) ◽  
pp. 122-126 ◽  
Author(s):  
A. Hanzalová ◽  
P. Bartoš ◽  
T. Sumíková
Keyword(s):  
Czech Republic ◽  
Leaf Rust ◽  
Puccinia Triticina ◽  
Near Isogenic Lines ◽  
Wheat Leaf Rust ◽  
Chain Reaction ◽  
The Czech Republic ◽  
Wheat Leaf ◽  
Polymerase Chain ◽  
Different Parts

In 2012–2015 the virulence of the wheat leaf rust (Puccinia triticina Eriks.) population was studied on Thatcher near-isogenic lines with Lr1, Lr2a, Lr2b, Lr2c, Lr3a, Lr9, Lr10, Lr11, Lr13, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28. Samples of leaf rust were obtained from different parts of the Czech Republic. A total of 163 wheat leaf rust isolates were analysed. No virulence for the resistance gene Lr9 was found. Virulence for Lr19 was found only in one isolate in 2015. A lower frequency of virulence to Lr24, Lr2a, 2b, 2c and Lr28 was also observed. The presence of Lr10, Lr24, Lr26, Lr28 and Lr37 in registered cultivars was detected by polymerase chain reaction (PCR) molecular markers.

scholarly journals Molecular identification of wheat leaf rust resistance genes in sixty Chinese wheat cultivars

2018 ◽  
Vol 54 (No. 1) ◽  
pp. 1-8
Author(s):  
Z. Ren ◽  
Z. Li ◽  
L. Shi ◽  
X. Wang ◽  
L. Zhu ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Wheat Cultivars ◽  
Wheat Leaf Rust ◽  
Progress Curve ◽  
Wheat Leaf ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Common wheat (Triticum aestivum L.) is the major crop cultivated in Xinjiang and Anhui provinces of China. The climate in these two provinces is favourable for wheat leaf rust (Puccinia triticina) (Pt) infection. Here, we demonstrate a detailed investigation on the leaf rust resistance of 60 major wheat cultivars cultivated in these two regions. A mixture of high virulent Pt races (THTT, THTS, THTQ and PHPS) were used to phenotype all the collected wheat cultivars at an adult plant stage. Phenotypic disease severity (FDS) and the area under the disease progress curve (AUDPC) for each of these wheat cultivars were calculated. Among all the tested wheat cultivars, three cultivars (Xindong20, Xindong 29 and 99AR142-1) with the lowest FDS and AUDPC may carry major resistance genes. Twenty-seven cultivars (45% of the total tested ones) showed a relatively lower resistance with an average of 12.52% FDS and 126.3 AUDPC. Minor resistance or slow rusting genes may be present in this group of cultivars. Molecular markers for leaf rust resistance genes Lr1, Lr9, Lr19, Lr24, Lr26 and Lr34 were further used for the genotypic screening. Lr1, Lr19, Lr26 and Lr34 were detected in 19 (31.7%), 1 (1.7%), 12 (20%) and 6 (10%) wheat cultivars, respectively. Neither Lr9 nor Lr24 could be detected in any of the tested cultivars. These results will greatly improve wheat molecular breeding for leaf rust resistance in these areas.

scholarly journals Physiological specialization of wheat leaf rust (Puccinia triticina Eriks.) in the Czech Republic in 2009–2011

2013 ◽  
Vol 49 (No. 3) ◽  
pp. 103-108 ◽  
Author(s):  
A. Hanzalová ◽  
P. Bartoš ◽  
T. Sumíková
Keyword(s):  
Czech Republic ◽  
Leaf Rust ◽  
Puccinia Triticina ◽  
Wheat Leaf Rust ◽  
The Czech Republic ◽  
Wheat Leaf ◽  
Winter Wheat Cultivars ◽  
Almost All ◽  
Yield Trials ◽  
Different Parts

In 2009–2011 virulence of the wheat leaf rust population was studied on Thatcher near isogenic lines with Lr1, Lr2a, Lr2b, Lr2c, Lr3a, Lr9, Lr11, Lr13, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28. Samples of leaf rust were obtained in different parts of the Czech Republic. A total of 164 wheat leaf rust isolates were analysed. Resistance gene Lr9 was effective to 98% of all tested isolates. No virulence to Lr19 was found. Gene Lr24 was effective to 93% of isolates. A lower frequency of virulence to Lr2a, Lr2b and Lr28 was also observed. Recently registered cultivars were tested with six older and five most widespread leaf rust pathotypes at present. Winter wheat cultivars Carroll and Citrus were resistant to all tested older pathotypes at the seedling stage and they were also resistant to almost all pathotypes widespread at present. They displayed resistance also in official yield trials. Genes Lr34, Lr37, Lr10, Lr24 were determined in the tested cultivars by molecular markers.

scholarly journals Wheat leaf rust (Puccinia triticina Eriks.) virulence frequency and detection of resistance genes in wheat cultivars registered in the Czech Republic in 2016–2018

2020 ◽  
Vol 56 (No. 3) ◽  
pp. 87-92 ◽  
Author(s):  
Alena Hanzalová ◽  
Veronika Dumalasová ◽  
Ondřej Zelba
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Puccinia Triticina ◽  
Near Isogenic Lines ◽  
Wheat Cultivars ◽  
Wheat Leaf Rust ◽  
The Czech Republic ◽  
Lr Genes ◽  
Wheat Leaf ◽  
Virulence Frequency

In 2016–2018 virulence of the Czech wheat leaf rust population was studied on Thatcher near-isogenic lines, carrying different Lr genes, and 130 leaf rust isolates. Virulence to Lr9 was found only sporadically. Virulence frequency to Lr2a, Lr2b, Lr2c and Lr28 was lower than in previous years. All tested isolates were avirulent to Lr19. Lr24 conditioned resistance to majority of isolates. Nineteen recently registered Czech cultivars were tested with six isolates of the pathogen and Lr genes were postulated. Presence of genes Lr1, Lr10, Lr19, Lr24, Lr26, Lr28, Lr34 and Lr37 was tested by molecular markers. Lr37 prevailed, followed by Lr genes 10, 24, 28, 1 and 26; genes Lr19 and Lr34 were not determined.

scholarly journals Distribution of Pathotypes with Regard to Host Cultivars in French Wheat Leaf Rust Populations

2006 ◽  
Vol 96 (3) ◽  
pp. 264-273 ◽  
Author(s):  
Henriette Goyeau ◽  
Robert Park ◽  
Brigitte Schaeffer ◽  
Christian Lannou
Keyword(s):  
Leaf Rust ◽  
Bread Wheat ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Wheat Cultivars ◽  
Wheat Leaf Rust ◽  
Seedling Resistance ◽  
Wheat Leaf ◽  
Selection For

Isolates of wheat leaf rust collected from durum and bread wheat cultivars in France during 1999-2002 were analyzed for virulence on 18 Thatcher lines with single genes for leaf rust resistance (Lr genes). Sampling focused on the five most widely grown bread wheat cultivars (two susceptible and three resistant) to allow statistical comparison of diversity indexes between the cultivars. Leaf rust populations from durum and bread wheats were different. The diversity of the bread wheat leaf rust pathotypes, as measured by the Shannon index, ranged from 2.43 to 2.76 over the 4 years. Diversity for wheat leaf rust resistance was limited in the host since we postulated only seven seedling resistance genes in the 35 cultivars most widely grown during 1999-2002. Leaf rust populations were strongly differentiated for virulence within bread wheat cultivars, and diversity was higher on those that were resistant, mainly due to a more even distribution of virulence phenotypes than on susceptible cultivars. The pathogen population on the susceptible cv. Soissons was largely dominated by a single pathotype (073100), whereas all other pathotypes virulent on cv. Soissons either decreased in frequency or remained at a low frequency during the period studied. Several pathotypes including the most complex one were found only on resistant cultivars, even though most of them were virulent on the susceptible cv. Soissons. Specific interactions were necessary, but not always sufficient, to account for pathotype distribution and frequencies on the cultivars, suggesting that selection for virulence to host resistance genes is balanced by other selective forces including selection for aggressiveness.

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