Topographical mapping of sympathetic postganglionic innervation of mouse heart v1

Mapping Intimacies ◽

10.17504/protocols.io.b2ebqban ◽

2021 ◽

Author(s):

Ariege Bizanti ◽

Yuanyuan Zhang ◽

Kohlton Bendowski ◽

Jin Chen ◽

Mahyar Osanlouy

Keyword(s):

Tyrosine Hydroxylase ◽

Mouse Heart ◽

Topographical Organization ◽

Topographical Mapping ◽

Whole Mounts

This protocol describes the process of mapping the topographical organization of tyrosine hydroxylase immune reactive sympathetic postganglionic axons and terminals in the mouse heart. Hearts were removed and separated as whole mounts, then scanned using confocal or zeiss microscopy

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Mapping CGRP-IR innervation of male mice stomach with Neurolucida 360 v1

10.17504/protocols.io.bygmptu6 ◽

2021 ◽

Author(s):

Duyen not provided Nguyen ◽

Jichao Ma

Keyword(s):

Confocal Microscopy ◽

Calcitonin Gene Related Peptide ◽

Male Mice ◽

Muscular Layer ◽

Related Peptide ◽

Topographical Organization ◽

Calcitonin Gene ◽

Whole Mounts ◽

Immunoreactive Axons

This protocol describes the process of using Neurolucida 360 software to map the topographical organization of Calcitonin gene related peptide – immunoreactive axons and terminals in the muscular layer of mice stomach. Stomachs were removed, layers were separated and gone under immunohistochemistry as whole mounts, then scanned using confocal microscopy.

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Three-Dimensional Reconstruction Using Stereo Pairs from Serial Thick Sections

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100080249 ◽

1977 ◽

Vol 35 ◽

pp. 562-563

Author(s):

Robert Glaeser ◽

Thomas Bauer ◽

David Grano

Keyword(s):

Three Dimensional ◽

Dimensional Structure ◽

Serial Sections ◽

Thin Sections ◽

3 Dimensional ◽

Transmission Electron ◽

Freeze Dried ◽

Dimensional Reconstruction ◽

Stereo Imagery ◽

Whole Mounts

In transmission electron microscopy, the 3-dimensional structure of an object is usually obtained in one of two ways. For objects which can be included in one specimen, as for example with elements included in freeze- dried whole mounts and examined with a high voltage microscope, stereo pairs can be obtained which exhibit the 3-D structure of the element. For objects which can not be included in one specimen, the 3-D shape is obtained by reconstruction from serial sections. However, without stereo imagery, only detail which remains constant within the thickness of the section can be used in the reconstruction; consequently, the choice is between a low resolution reconstruction using a few thick sections and a better resolution reconstruction using many thin sections, generally a tedious chore. This paper describes an approach to 3-D reconstruction which uses stereo images of serial thick sections to reconstruct an object including detail which changes within the depth of an individual thick section.

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The Fine Structure of Irradiated Mouse Heart

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100090907 ◽

1976 ◽

Vol 34 ◽

pp. 184-185

Author(s):

Vivian V. Yang ◽

S. Phyllis Stearner

Keyword(s):

Microscopic Level ◽

Ultrastructural Changes ◽

Mouse Heart ◽

Blood Vessel Wall ◽

Histopathological Changes ◽

Light Microscopic Level ◽

Γ Rays ◽

Fission Neutrons ◽

Total Body

The heart is generally considered a radioresistant organ, and has received relatively little study after total-body irradiation with doses below the acutely lethal range. Some late damage in the irradiated heart has been described at the light microscopic level. However, since the dimensions of many important structures of the blood vessel wall are submicroscopic, investigators have turned to the electron microscope for adequate visualization of histopathological changes. Our studies are designed to evaluate ultrastructural changes in the mouse heart, particularly in the capillaries and muscle fibers, for 18 months after total-body exposure, and to compare the effects of 240 rad fission neutrons and 788 rad 60Co γ-rays.Three animals from each irradiated group and three control mice were sacrificed by ether inhalation at 4 days, and at 1, 3, 6, 12, and 18 months after irradiation. The thorax was opened and the heart was fixed briefly in situwith Karnofsky's fixative.

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