Mapping CGRP-IR innervation of male mice stomach with Neurolucida 360 v1

Male Mice ◽

Muscular Layer ◽

Related Peptide ◽

Topographical Organization ◽

Calcitonin Gene ◽

Whole Mounts ◽

This protocol describes the process of using Neurolucida 360 software to map the topographical organization of Calcitonin gene related peptide – immunoreactive axons and terminals in the muscular layer of mice stomach. Stomachs were removed, layers were separated and gone under immunohistochemistry as whole mounts, then scanned using confocal microscopy.

Demonstration of calcitonin gene-related peptide immunoreactive axons contacting dynorphin A(1–8) immunoreactive spinal neurons in a rat model of peripheral inflammation and hyperalgesia

Brain Research ◽

10.1016/0006-8993(88)90213-2 ◽

1988 ◽

Vol 475 (1) ◽

pp. 168-172 ◽

Cited By ~ 42

Author(s):

Osamu Takahashi ◽

Richard J. Traub ◽

M.A. Ruda

Keyword(s):

Rat Model ◽

Peripheral Inflammation ◽

Spinal Neurons ◽

Dynorphin A ◽

Related Peptide ◽

Calcitonin Gene ◽

Regrowth of calcitonin gene-related peptide (CGRP) immunoreactive axons from the chronically injured rat spinal cord into fetal spinal cord tissue transplants

Neuroscience Letters ◽

10.1016/0304-3940(89)90108-0 ◽

1989 ◽

Vol 103 (3) ◽

pp. 253-258 ◽

Cited By ~ 37

Author(s):

John D. Houle ◽

Paul J. Reier

Keyword(s):

Spinal Cord ◽

Spinal Cord Tissue ◽

Rat Spinal Cord ◽

Related Peptide ◽

Tissue Transplants ◽

Calcitonin Gene ◽

Ultrastructural demonstration of synaptic connections between calcitonin gene-related peptide immunoreactive axons and dynorphin A(1–8) immunoreactive dorsal horn neurons in a rat model of peripheral inflammation and hyperalgesia

Peptides ◽

10.1016/0196-9781(90)90157-z ◽

1990 ◽

Vol 11 (6) ◽

pp. 1233-1237 ◽

Cited By ~ 19

Author(s):

Osamu Takahashi ◽

Sadao Shiosaka ◽

Richard J. Traub ◽

M.A. Ruda

Keyword(s):

Dorsal Horn ◽

Rat Model ◽

Peripheral Inflammation ◽

Synaptic Connections ◽

Dynorphin A ◽

Dorsal Horn Neurons ◽

Related Peptide ◽

Calcitonin Gene ◽

Calcitonin gene-related peptide-and substance-P-immunoreactive axons in the nucleus gracilis of the rat

Neuroscience Research Supplements ◽

10.1016/0921-8696(86)90244-6 ◽

1986 ◽

Vol 3 ◽

pp. S117

Author(s):

Hiroshi Takagi ◽

Yumiko Morishima ◽

Yoshinori Kawai ◽

Shiro Mori

Keyword(s):

Substance P ◽

Nucleus Gracilis ◽

Related Peptide ◽

Calcitonin Gene ◽

Parabrachial Origin of Calcitonin Gene-Related Peptide-Immunoreactive Axons Innervating Meynert's Basal Nucleus

Experimental Neurology ◽

10.1006/exnr.1999.7050 ◽

1999 ◽

Vol 157 (2) ◽

pp. 268-276 ◽

Cited By ~ 3

Author(s):

Elizabeth Knyihár-Csillik ◽

István Boncz ◽

Gyula Sáry ◽

János Nemcsók ◽

Bertalan Csillik

Keyword(s):

Basal Nucleus ◽

Related Peptide ◽

Calcitonin Gene ◽

Second Place — Resident Basic Science Award 1994: Subcellular Innervation Patterns of the Calcitonin Gene-Related Peptidergic Efferent Terminals in the Chinchilla Vestibular Periphery

Otolaryngology ◽

10.1177/019459989411100402 ◽

1994 ◽

Vol 111 (4) ◽

pp. 385-395 ◽

Cited By ~ 18

Author(s):

Akira Ishiyama ◽

Ivan Lopez ◽

Phillip A. Wackym

Keyword(s):

Vestibular System ◽

Hair Cells ◽

Type I ◽

Type Ii ◽

Primary Afferent ◽

Related Peptide ◽

Calcitonin Gene ◽

Innervation Patterns ◽

We examined the ultrastructural distribution of calcitonin gene-related peptide immunoreactivity in the peripheral vestibular system of the chinchilla to study the Innervation patterns of this efferent neuropeptide. Immunoelectron microscopic localization of calcitonin gene-related peptide immunoreactive terminals in the maculae and cristae revealed an extensive innervation pattern on the afferent vestibular pathway. Calcitonin gene-related peptide immunoreactive terminals made synaptic contacts with the unmyelinated portions of the primary afferent vestibular dendrites innervating both type I and type II hair cells. Abundant synaptic contact between calcitonin gene-related peptide immunoreactive terminals and the chalices surrounding type I hair cells was observed. Direct contact between calcitonin gene-related peptide immunoreactive terminals and type II hair cells was observed. In addition, vesiculated efferent terminals without calcitonin gene-related peptide Immunoreactivity were seen synapsing on the chalices of type II hair cells and on the surrounding type I hair cells. The primary afferent somata in the vestibular ganglion of Scarpa did not contain calcitonin gene-related peptide Immunoreactivity. Unmyelinated calcitonin gene-related peptide immunoreactive axons passed among the primary afferent fibers in Scarpa's ganglion, and these fibers continued through the subepithelial regions of the vestibular end-organs. The calcitonin gene-related peptide immunoreactive axons ramified to produce numerous calcitonin gene-related peptide immunoreactive terminals throughout the neurosensory epithelium of the maculae and cristae. These data suggest that calcitonin gene-related peptide—mediated modulation of the afferent vestibular system is functionally important.