scholarly journals Superoxide Radical-scavenging Activity v1 (protocols.io.bdaxi2fn)

protocols.io ◽  
2020 ◽  
Author(s):  
Jing Xu
Keyword(s):  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity

scholarly journals Radical scavenging activity of crude polysaccharides from Camellia sinensis

2011 ◽  
Vol 63 (3) ◽  
pp. 717-721
Author(s):  
Fan Yang ◽  
Zhiwei Yang ◽  
Jianbo Xiao
Keyword(s):  
Molecular Weight ◽  
Free Radical ◽  
Camellia Sinensis ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Concentration Dependent Manner ◽  
Molecular Weights

A preparation of crude polysaccharides (TPS) was isolated from Camellia sinensis by precipitation and ultrafiltration. TPS1, TPS2, and TPS3 had molecular weights of 240, 21.4, and 2.46 kDa, respectively. The radical scavenging activities of TPS were evaluated by DPPH free radical, hydroxyl radical and superoxide radical scavenging. These results revealed that TPS exhibited strong radical scavenging activity in a concentration-dependent manner. TPS3 with lowest molecular weight showed a higher radical scavenging activity.

scholarly journals Effect of Temperature, pH, Enzyme to Substrate Ratio, Substrate Concentration and Time on the Antioxidative Activity of Hydrolysates from Goat Milk Casein by Alcalase

2016 ◽  
Vol 20 (2) ◽  
pp. 29-38 ◽  
Author(s):  
Guowei Shu ◽  
Bowen Zhang ◽  
Qian Zhang ◽  
Hongchang Wan ◽  
Hong Li
Keyword(s):  
Substrate Concentration ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Goat Milk ◽  
Scavenging Activity ◽  
Antioxidant Peptides ◽  
Hydrolysis Time ◽  
Hydrolysis Temperature ◽  
Milk Casein

Abstract The effect of hydrolysis temperature (45, 50, 55, 60 and 65°C), pH (7.0, 7.5, 8.0, 8.5 and 9.0), enzyme to substrate (E/S) ratio (1.0, 1.5, 2.0, 2.5 and 3.0%), substrate concentration (2, 3, 4, 5 and 6%) and hydrolysis time (30-240min) on antioxidant peptides hydrolysated from goat’s milk casein by Alcalase was investigated using single factor experiment. In order to obtain high DPPH radical-scavenging activity, metal-chelating activity and superoxide radical scavenging activity, the optimal conditions were hydrolysis time of 150 min, temperature of 50°C, pH 8.0, E/S ratio of 2.0% and substrate concentration of 4.0%. The hydrolysis time, hydrolysis temperature, pH, E/S ratio and substrate concentration had a significant influence on degree of hydrolysis, metal-chelating activity, DPPH and superoxide radical scavenging activity on casein hydrolysate of goat milk by Alcalase, the results were beneficial for further provide theoretical basis for production of antioxidant peptides.

Isolation and identification of antioxidant peptides derived from whey protein enzymatic hydrolysate by consecutive chromatography and Q-TOF MS

2013 ◽  
Vol 80 (3) ◽  
pp. 367-373 ◽  
Author(s):  
Qiu-Xiang Zhang ◽  
Hui Wu ◽  
Yu-Fang Ling ◽  
Rong-Rong Lu
Keyword(s):  
Whey Protein ◽  
Superoxide Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Gel Filtration ◽  
Scavenging Activity ◽  
Antioxidant Peptides ◽  
Isolation And Identification ◽  
Tof Ms

To isolate and identify antioxidant peptides from enzymatically hydrolysed whey protein, whey protein isolate was hydrolysed by different protease (trypsin, pepsin, alcalase 2·4L, promatex, flavourzyme, protease N). The hydrolysate generated by alcalase 2·4L had the highest antioxidant activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, superoxide radicals and in a linoleic acid peroxidation system induced by Fe2+. The IC50 values of DPPH and superoxide radical scavenging activities of the hydrolysate decreased significantly (6·89 and 38·88%, respectively) after treatment with macroporous adsorption resin. Seven different peptides showing strong antioxidant activities were isolated from the hydrolysate using consecutive chromatographic methods including gel filtration chromatography and high-performance liquid chromatography. The molecular mass and amino acids sequences of the purified peptides were determined using a Quadrupole time-of-flight mass spectrometer (Q-TOF MS). One of the antioxidative peptides, Trp–Tyr–Ser–Leu, displayed the highest DPPH radical scavenging activity (IC50=273·63 μm) and superoxide radical scavenging activity (IC50=558·42 μm). These results suggest that hydrolysates from whey proteins are good potential source of natural antioxidants.

scholarly journals FREE-RADICAL SCAVENGING ACTIVITY LEAF EXTRACT OF LITSEA LAEVIGATA GAMBLE

2019 ◽  
pp. 96-103
Author(s):  
S. SUJATHA ◽  
T. SEKAR
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Leaf Extract ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Metal Chelating

Objective: In the present study, antioxidant activity in the leaf of the pet-ether, chloroform, acetone and methanolic extracts from Litsea laevigata Gamble. Leaf was investigated by employing established in vitro studies. L. laevigata belongs to the Lauraceae family. Methods: The capability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals were determined by in vitro antioxidant assays using 2,2-diphenyl-2-picrylhydrazyl free radical (DPPH.) scavenging, reducing power assay, superoxide radical (O2*-) scavenging activity, phosphomolybdenum assay, FRAP, ABT and metal chelating activity were performed to know the antioxidant potency of the plant extract of leaves of L. laevigata. Results: Results are evaluated higher in leaf extract of L. laevigata recorded total phenol, total flavonoid, and tannin. The present state of work was designed to evaluate the phytochemical, antioxidant in the plant leaf extracts of L. laevigata. The plant L. laevigata methanolic extract of leaf showed greater IC50 antioxidant activity of DPPH assay (5.264 µg/ml) and compare to other extract, higher phosphomolybdenum reduction (164.36 mg/g), better Reducing power activity leaf in methanol (0.711%), higher ferric reducing power (4060.66MmolFe(II)E/mg), and higher in superoxide radical scavenging activity in (78.12 mg/ml). However, the better metal chelating ability was shown by the water extracts of the leaf (5.145 EDTAE/100g) compared to other solvent extracts. Conclusion: The result indicates the total phenol and antioxidant activity potential of L. laevigata.

scholarly journals Effect Of Five Proteases Including Alcalase, Flavourzyme, Papain, Proteinase K And Trypsin On Antioxidative Activities Of Casein Hydrolysate From Goat Milk

2015 ◽  
Vol 19 (2) ◽  
pp. 65-74 ◽  
Author(s):  
Guowei Shu ◽  
Qian Zhang ◽  
He Chen ◽  
Hongchang Wan ◽  
Hong Li
Keyword(s):  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Casein Hydrolysate ◽  
Goat Milk ◽  
The Body ◽  
Proteinase K ◽  
Scavenging Activity ◽  
Dpph Scavenging ◽  
Casein Hydrolysates

Abstract Oxidation was related to the pathogenesis of human diseases. Adequate intake of antioxidant activity of food can reduce the levels of free radicals, prevent lipid peroxidation, and help the body against diseases. In the paper, casein from goat milk was hydrolyzed by five commercial proteases, namely, Alcalase, flavourzyme, papain, proteinase K and trypsin. The antioxidant activities of casein hydrolysates were assessed by evaluating hydrolysis degree, DPPH radical-scavenging activity, metal-chelating activity and superoxide radical scavenging activity. The results showed as follows: the DH of proteinase K, Alcalase, and trypsin were higher significantly than those of papain and flavourzyme. The Fe2+-chelation activity and superoxide radical scavenging activity of casein hydrolysates from goat milk by Alcalase was higher than the others, the DPPH scavenging activities of casein hydrolysates by Alcalase and papain was higher than the others and the DPPH scavenging activities by Alcalase and papain had no significant diffierence (p<0.05), so the optimal proteinase for hydrolysis casein from goat milk to produce antioxidant peptide was Alcalase.

Antioxidant Properties of R-Phycoerythrin from Red Alga Spyridia filamentosa (Wulfen) Harvey Collected on the Pudumadam Coast

2020 ◽  
Vol 12 (4) ◽  
pp. 489-498
Author(s):  
A. Brabakaran ◽  
S. Venkatesan ◽  
K. R. Jayappriyan ◽  
L. Selva Roselin ◽  
N. Thangaraju
Keyword(s):  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Fluorescence Emission ◽  
Scavenging Activity ◽  
Hydroxyl Radical Scavenging Activity ◽  
Uv Visible ◽  
Dpph Radical Scavenging ◽  
Spyridia Filamentosa

Natural antioxidants are found in algae, are important biologically active compounds that involve in protecting the cells from oxidation thereby used in defense mechanism on ageing processes and various diseases. In this study, R-Phycoerythrin (R-PE) was extracted from Spyridia filamentosa and was assessed for their antioxidant properties. The antioxidising property of decontaminated R-PE has been evaluated by measuring the free radical scavenging activity by DPPH radical scavenging assay, ABTS radical scavenging activity, hydroxyl radical scavenging activity, superoxide radical (O2) scavenging assay, nitric oxide scavenging activity and phosphomolybdenum reduction assay. The purified R-PE extract was subjected to structural analysis by UV-Visible spectroscopy, fluorescence emission spectroscopy and infrared spectroscopy. The UV-Visible spectrum showed a peak at 496 nm, 543 nm and 564 nm and fluorescence emission spectrum of R-PE was measured 575 nm which confirmed the presence of R-Phycoerythrin. The IR spectral data showed the presence of aromatic constituents containing amide group in the protein. DPPH radical scavenging assay showed the highest antioxidant potential with an IC50 value of 128.25 μg/ml. The radical scavenging activity of Nitric oxide showed an IC50 value of 87.85 μg/ml, Phosphomolybdenum reduction assay showed an RC50 value of 61.45 μg/ml. The hydroxyl radical scavenging activity showed IC50 value of 34.56 μg/ml and Superoxide radical (O2) scavenging assay showed IC50 value of 18.58 μg/ml. The ABTS radical scavenging activity showed the lowest antioxidant potential of 3.13 μg/ml. The free radical scavenging property of R-Phycoerythrin (R-PE) concentrate of Spyridia filamentosa demonstrate that it has high antioxidant potency and it could be considered as utilitarian nourishment with essential pharmaceutical and biological importance.

scholarly journals EVALUATION OF IN VITRO ANTIOXIDANT ACTIVITY OF A MEDICINAL HERB, WEDELIA CHINENSIS (OSBECK) MERRILL.

2018 ◽  
Vol 11 (10) ◽  
pp. 433
Author(s):  
Rehana Banu H ◽  
Nagarajan N
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Total Antioxidant ◽  
Dpph Radical Scavenging ◽  
In Vitro Antioxidant Activity

Objective: The objective is to evaluate the antioxidant activity of the methanolic leaf extract of Wedelia chinensis.Methods: In vitro antioxidant activity was evaluated by studying 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, superoxide radical scavenging activity, ferric reducing ability of plasma (FRAP) scavenging activity, hydroxyl radical scavenging activity, metal chelating activity, and total antioxidant activity. Ascorbic acid, BHT, quercetin, and EDTA were used as standards for the experiments.Results: Inhibition concentration50 values observed for DPPH radical scavenging, superoxide radical scavenging, and hydroxyl radical scavenging activities were determined to be 62.5, 769.23, and 617.28 μg/ml, respectively and that of FRAP scavenging, metal chelating, and total antioxidant activities were found to be 343 μmol Fe (II)/g, 79.56 mg EDTA/g extract, and 398.17 mg ascorbic acid eq/g extract, respectively.Conclusion: The results clearly indicate that methanolic leaf extract of the study species W. chinensis is effective in scavenging free radicals and has the potential to be a powerful antioxidant.

Sign in / Sign up

Export Citation Format

Share Document