Human embryonic gonad dissociation with Collagenase & Trypsin v2

protocols.io ◽  
2021 ◽  
Author(s):  
Carmen Sancho ◽  
Regina Hoo ◽  
Roser Vento-Tormo
Keyword(s):  
Embryonic Gonad

scholarly journals Choriocarcinoma metastatic to the skin: A rare occurrence associated with dismal outcome

Rare Tumors ◽  
2021 ◽  
Vol 13 ◽  
pp. 203636132110397
Author(s):  
Mousa ElKhaldi ◽  
Rakan Radi ◽  
Maysa Al-Hussaini
Keyword(s):  
Back Pain ◽  
Germ Cell ◽  
Lower Back Pain ◽  
Germ Cell Tumors ◽  
Cutaneous Metastasis ◽  
Hematogenous Metastasis ◽  
Rare Occurrence ◽  
Lower Back ◽  
Embryonic Gonad ◽  
Work Up

Germ cell tumors (GCTs) are a histologically heterogeneous group of tumors that arise from the primitive germ cell of the embryonic gonad. Choriocarcinoma is a variant of GCTs that is prone to hematogenous metastasis to the liver, lung, and brain. Cutaneous metastasis in choriocarcinoma is rarely encountered with only a few cases reported in literature. We report the case of a 28-year-old male presenting with lower back pain that, upon further work-up, was diagnosed with pure choriocarcinoma of the testes. Around 9 months after his initial presentation, he developed a cutaneous back lesion. Microscopic examination confirmed the presence of choriocarcinoma composed of mononuclear cytotrophoblasts which interweave with multinucleated syncytiotrophoblasts. The patient passed away 3 weeks after the onset of cutaneous metastasis.

Role of mammalian Y chromosome in sex determination

1988 ◽  
Vol 322 (1208) ◽  
pp. 63-72 ◽  
Keyword(s):  
Cell Differentiation ◽  
Sex Determination ◽  
Y Chromosome ◽  
Sertoli Cell ◽  
Sertoli Cells ◽  
Sex Reversal ◽  
Testicular Development ◽  
Chimeric Mouse ◽  
Embryonic Gonad

It has long been assumed that the mammalian Y chromosome either encodes, or controls the production of, a diffusible testis-determining molecule, exposure of the embryonic gonad to this molecule being all that is required to divert it along the testicular pathway. My recent finding that Sertoli cells in XX ↔ XY chimeric mouse testes are exclusively XY has led me to propose a new model in which the Y acts cell-autonomously to bring about Sertoli-cell differentiation. I have suggested that all other aspects of foetal testicular development are triggered by the Sertoli cells without further Y-chromosome involvement. This model thus equates mammalian sex determination with Sertoli-cell determination. Examples of natural and experimentally induced sex reversal are discussed in the context of this model.

scholarly journals Role of autonomous and non-autonomous sex determination signals in sexually dimorphic development of the Drosophila embryonic gonad

2007 ◽  
Vol 306 (1) ◽  
pp. 313 ◽  
Author(s):  
N.R. Crnkovich ◽  
T.J. DeFalco ◽  
S Le Bras ◽  
A.L. Casper ◽  
M.B. Van Doren
Keyword(s):  
Sex Determination ◽  
Sexually Dimorphic ◽  
Embryonic Gonad

scholarly journals The Rhox Homeobox Gene Family Shows Sexually Dimorphic and Dynamic Expression During Mouse Embryonic Gonad Development1

2008 ◽  
Vol 79 (3) ◽  
pp. 468-474 ◽  
Author(s):  
Hinda Daggag ◽  
Terje Svingen ◽  
Patrick S. Western ◽  
Jocelyn A. van den Bergen ◽  
Peter J. McClive ◽  
...  
Keyword(s):  
Gene Family ◽  
Homeobox Gene ◽  
Sexually Dimorphic ◽  
Embryonic Gonad ◽  
Dynamic Expression

Identification of genes controlling germ cell migration and embryonic gonad formation in Drosophila

Development ◽  
1998 ◽  
Vol 125 (4) ◽  
pp. 667-678 ◽  
Author(s):  
L.A. Moore ◽  
H.T. Broihier ◽  
M. Van Doren ◽  
L.B. Lunsford ◽  
R. Lehmann
Keyword(s):  
Cell Migration ◽  
Germ Cell ◽  
Germ Cells ◽  
Cell Types ◽  
Drosophila Embryo ◽  
Specific Cell ◽  
Embryonic Patterning ◽  
Phenotypic Analysis ◽  
Embryonic Gonad ◽  
Germ Cell Migration

Gonadogenesis in the Drosophila embryo is a complex process involving numerous cellular migratory steps and cell-cell interactions. The mechanisms guiding germ cells to move through, recognize and adhere to specific cell types are poorly understood. In order to identify genes that are required for these processes, we have conducted an extensive mutagenesis of the third chromosome and screened for mutations disrupting germ cell migration at any point in embryonic development. Phenotypic analysis of these mutants demonstrates that germ cell migration can be broken down into discrete developmental steps, with each step requiring a specific set of genes. Many of these genes are involved in the development of gonadal mesoderm, the tissue that associates with germ cells to form the embryonic gonad. Moreover, mutations that we isolated affecting embryonic patterning as well as germ cell migration suggest that the origin of gonadal mesoderm lies within the eve domain of the developing mesoderm.

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