Making electro-competent cells v1

The present paper addresses anatomically resolved protein networks by using the Imaging Cycler Microscopy (ICM/TIS) [1,2]. ICM is capable of resolving protein networks in intact anatomical structures at a power of combinatorial molecular resolution of 65,553k , where k is the number of co-mapped proteins, e.g. 100 proteins [1-4]. This method provides insight into the laws of the spatial communication of large protein networks in health and disease, which is essential for new therapy options in diseases.

Download Full-text

Mapping of the pneumococcus chromosome: differences between recipient strains varying inhexproperty and the location of theopt-r2gene

Genetics Research ◽

10.1017/s0016672300018127 ◽

1979 ◽

Vol 33 (1) ◽

pp. 1-14 ◽

Cited By ~ 1

Author(s):

L. O. Butler ◽

G. Nicholas ◽

R. W. Grist

Keyword(s):

Linkage Studies ◽

Chromosome Map ◽

Competent Cells ◽

Chromosome Arm

SUMMARYTransformation studies in pneumococcus had shown that loci determining the resistance to erythromycin and streptomycin were unlinked when strain Cl3 was recipient but linked when strain SIII-I was recipient. This phenomenon also applies to other pairs of markers studied in these two recipients, no matter whether the transforming DNA was derived from strain Cl3 or strain SIII-I. Other differences between the two recipient strains were also revealed. Whereas competent cultures of strain Cl3 were composed of all competent cells, which was in agreement with previous reports of pneumococcal cultures, strain SIII-I normally gave a maximum average of 28% competent cells. Strain SIII-I was unstable, since on repeated sub-culturing the competence peak profile changed and the value of ‘fcq’ increased. These properties were reflected in the twohex−strains 401 and R6x which were found to be similar to the ‘altered SIII-I’ strain. The results from the linkage studies have been applied to the chromosome map and have placed theopt-r2gene in thestr-r41-containing chromosome arm.

Download Full-text

Improved long-term cryostorage of Escherichia coli competent cells using trehalose

Biotechnology Letters ◽

10.1023/b:bile.0000045659.41621.de ◽

2004 ◽

Vol 26 (20) ◽

pp. 1593-1594 ◽

Cited By ~ 1

Author(s):

Taeho Ahn ◽

Seong Su Kang ◽

Chul-Ho Yun

Keyword(s):

Escherichia Coli ◽

Competent Cells

Download Full-text

DNA ‘Velcro’ to Interface Electron Transfer-Competent Cells with Electrodes

ECS Meeting Abstracts ◽

10.1149/ma2021-02471415mtgabs ◽

2021 ◽

Vol MA2021-02 (47) ◽

pp. 1415-1415

Author(s):

Ariel L Furst

Keyword(s):

Electron Transfer ◽

Competent Cells

Download Full-text

Letters to the Editor

PEDIATRICS ◽

10.1542/peds.40.3.469a ◽

1967 ◽

Vol 40 (3) ◽

pp. 469-470

Author(s):

REBECCA H. BUCKLEY

Keyword(s):

Maternal Origin ◽

Host Reaction ◽

Letters To The Editor ◽

Competent Cells

Dr. Miller's point (suggested by the work of Kadowaki, et al.) is well taken that immunologically competent cells of maternal origin could likely survive in infants with thymic alymphoplasia and perhaps function as immunoglobulin-producing cells in the infant. We did not touch upon this point in our discussion because we had no karyotyping data on our patient and also because there was nothing present clinically to suggest a graft vs host reaction. In the case of Kadowaki, et al., the patient failed to grow after 3 months of age and was notably "runted."

Download Full-text