Lipidomic analysis of tissue culture cells, tissues, and purified organelles v1
Keyword(s):
Lipids are a class of molecules that have roles in energy storage, plasma membrane integrity, and signaling events. To gain more understanding of the functions and roles that lipids play in biology, researchers employ discovery analytical approaches, such as mass spectrometry (MS)-based lipidomics. The main objective of this protocol is to provide directive on how to extract lipids from plasma, cells, tissue, and purified organelles for analysis by liquid chromatography (LC)-MS. This analysis will typically yield quantitative data for more than 200 lipids, depending on the sample type analyzed, across a range of lipid classes: phospholipids, cardiolipins, sphingolipids, di- and triacylglyerols, and cholesteryl-esters.
1969 ◽
Vol 27
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pp. 376-377
1993 ◽
Vol 51
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pp. 342-343
1976 ◽
Vol 18
(3)
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pp. 1147-1150
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1982 ◽
Vol 47
(5)
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pp. 1530-1536
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1973 ◽
Vol 248
(19)
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pp. 6738-6743
1976 ◽
Vol 251
(21)
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pp. 6747-6756
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1979 ◽
Vol 254
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pp. 3935-3946
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1978 ◽
Vol 253
(12)
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pp. 4408-4418
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1978 ◽
Vol 253
(17)
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pp. 6125-6131
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