Preparation of oleylethylene glycol-sreptavidin surfaces for SPR v2

2021 ◽  
Author(s):  
Dave Fernig ◽  
Dunhao Su
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Science ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Surface Coverage ◽  
Specific Binding ◽  
Gold Surface ◽  
Self Assembled Monolayer ◽  
Biomimetic Surfaces ◽  
Surface Functionalisation

Surface plasmon resonance uses gold surfaces for sensing. Manufacturers provide a range of pre-functionalised surfaces, but these are often prone to non-specific binding problems. In other surface science sensing techniques a range of surface functionalisation approaches have been described. Here, the preparation of a self-assembled monolayer (SAM) of a thiolated oleyl ethylene glycol, incorporating a defined mole % of biotinylated ligands , on a gold surface is described. This allows the formation of a streptavidin layer on the SAM with control over the average surface coverage of streptavidin. Biotinylated ligands can then be immobilised on the streptavidin. Such surfaces have proved to be very resistant to non-specific binding and they are easily implemented on the sensor surfaces of commercial (surface plasmon resonance) SPR instruments. This is adapted from a published method: Migliorini, E. et al. Well-defined biomimetic surfaces to characterize glycosaminoglycan-mediated interactions on the molecular, supramolecular and cellular levels. Biomaterials (2014) doi:10.1016/j.biomaterials.2014.07.017.

Localized and Propagating Surface Plasmon Resonance Sensors: A Study Using Carbohydrate Binding Protein

2005 ◽  
Vol 876 ◽  
Author(s):  
Chanda Yonzon ◽  
Richard P. Van Duyne
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Specific Binding ◽  
Gold Surface ◽  
Optical Biosensor ◽  
Localized Surface Plasmon ◽  
Carbohydrate Binding ◽  
Binding Studies ◽  
Assembled Monolayers

AbstractThis work encompasses a comparative analysis of the properties of two optical biosensor platforms: (1) the propagating surface plasmon resonance (SPR) sensor based on a planar, thin film gold surface and (2) the localized surface plasmon resonance (LSPR) sensor based on surface confined Ag nanoparticles fabricated by nanosphere lithography. The binding of Concanavalin A (ConA) to mannose-functionalized self-assembled monolayers (SAMs) is chosen to illustrate the similarities and the differences of these sensors. A comprehensive set of non-specific binding studies demonstrate that the single transduction mechanism is due to the specific binding of ConA to the mannose-functionalized surface. Finally, an elementary (2x1) multiplexed version of a LSPR carbohydrate sensing chip to probe the simultaneous binding of ConA to mannose and galactose-functionalized SAMs is also demonstrated.

scholarly journals Application of Different Lipid Surfaces to Monitor Protein–Membrane Interactions by Surface Plasmon Resonance Spectroscopy

2002 ◽  
Vol 16 (3-4) ◽  
pp. 271-279 ◽  
Author(s):  
Torsten Fischer ◽  
Ivan I. Senin ◽  
Pavel P. Philippov ◽  
Karl-Wilhelm Koch
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Gold Surface ◽  
Test System ◽  
Sensor Chip ◽  
Resonance Spectroscopy ◽  
Surface Plasmon Resonance Spectroscopy ◽  
Self Assembled Monolayer ◽  
Self Assembled

Planar lipid bilayers on sensor chip surfaces have become useful tools to study membrane bound processes by surface plasmon resonance spectroscopy. We immobilized phospholipids on sensor chips by different approaches. First, a self-assembled monolayer of octadecylmercaptan was formed on a blank gold surface and subsequent addition of phospholipids led to formation of a heterobilayer. Second, a self-assembled monolayer of mercaptoundecanoic acid was formed on a gold surface, the carboxy groups of mercaptoundecanoic acid were activated and covalently linked to phosphatidylethanolamine. Addition of phospholipids then led to a bilayer with phosphatidylethanolamine as the lower leaflet. Third, a hydrophobic sensor chip (L1, BIAcore) was used as a binding matrix for phospholipids. These lipid surfaces were tested, whether they are suitable to study proteinamembrane interactions. As biological test system we used the Ca2+-myristoyl-switch of the neuronal Ca2+-binding protein recoverin. All three surfaces were sufficiently stable to monitor the Ca2+-dependent binding of recoverin to membranes.

Grating-Coupled Surface-Plasmon-Resonance Biosensor Discs with a C-Type Fluidic Channel for Monitoring Growth of Self-Assembled Monolayer

2013 ◽  
Vol 284-287 ◽  
pp. 2069-2074 ◽  
Author(s):  
Jenq Nan Yih ◽  
Kuo Chi Chiu ◽  
Sheng Yu Chou ◽  
Chih Ming Lin ◽  
Yung Sung Lan ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Gold Surface ◽  
Measuring System ◽  
Response Curves ◽  
Self Assembled Monolayer ◽  
Fluidic Channel ◽  
Promising Tool ◽  
Self Assembled

In this paper, we present a grating-coupled surface plasmon resonance (SPR) biosensor with a c-type fluidic channel on a grating disc. For obtaining the kinetic information of molecular interaction, we combined a c-type fluidic channel on disc to drive testing solution forward by gravity via turning the grating disc. The disc biosensor can react with probe molecules on the different sensing surfaces in the following time. The repeatability testing shows the resolution of angle of the measuring system was enhanced by threefold comparing to that without the automation improvement. In order to identify the accuracy of angle changes of SPR due to the chemical reaction on surface, experiment for monitoring a growth self-assembled monolayer (SAM) immobilized on gold surface was performed. The measured response curves show that the saturation time of the SAM formation, which thickness is smaller than 2 nm, is roughly 5 hours when 1 mM MHDA growing on gold at room temperature. The demonstration reveals that the disc biosensor with c-type fluidic channels can be a promising tool for a kinetic analysis of bimolecular interaction without any external fluid pumping systems.

scholarly journals Molecular Monolayer Sensing Using Surface Plasmon Resonance and Angular Goos-Hänchen Shift

Sensors ◽  
2021 ◽  
Vol 21 (13) ◽  
pp. 4593
Author(s):  
Cherrie May Olaya ◽  
Norihiko Hayazawa ◽  
Maria Vanessa Balois-Oguchi ◽  
Nathaniel Hermosa ◽  
Takuo Tanaka
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Incident Beam ◽  
Expected Value ◽  
Beam Waist ◽  
Self Assembled Monolayer ◽  
Hydrophilic Nature ◽  
Small Change ◽  
Made In

We demonstrate potential molecular monolayer detection using measurements of surface plasmon resonance (SPR) and angular Goos-Hänchen (GH) shift. Here, the molecular monolayer of interest is a benzenethiol self-assembled monolayer (BT-SAM) adsorbed on a gold (Au) substrate. Excitation of surface plasmons enhanced the GH shift which was dominated by angular GH shift because we focused the incident beam to a small beam waist making spatial GH shift negligible. For measurements in ambient, the presence of BT-SAM on a Au substrate induces hydrophobicity which decreases the likelihood of contamination on the surface allowing for molecular monolayer sensing. This is in contrast to the hydrophilic nature of a clean Au surface that is highly susceptible to contamination. Since our measurements were made in ambient, larger SPR angle than the expected value was measured due to the contamination in the Au substrate. In contrast, the SPR angle was smaller when BT-SAM coated the Au substrate due to the minimization of contaminants brought about by Au surface modification. Detection of the molecular monolayer acounts for the small change in the SPR angle from the expected value.

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