Effect of antioxidants agents on Dentinal Tubular Penetration of EndoREZ sealer on sodium hypochlorite-treated root canal dentin: A Scanning electron microscopic study v1

According to ISO, the canals were enlarged to a size 30, 6% taper with ProTaper gold (Dentsply Sirona, Ballaigues, Switzerland) by crown down technique (Singh et al., 2015).Irrigation was performed with Saline and 17% EDTA in Group I. After biomechanical preparation, groups II, III, IV, and V were irrigated with 5 mL-Sodium hypochlorite (5.25%) and 5 mL-EDTA (17%) for 1 minute. Irrigation was done on Groups III, IV, and V for an additional 10 minutes with 10% Ellagic acid, 5% Lycopene extract, and 5% Grape Seed Extract, respectively. After irrigation, the root canals were dried. The EndoREZ (Ultradent Products, Inc., South Jordan, Utah, USA) was used in the canals using a 25-size spreader (Mani Inc., Tochigi, Japan), followed by warm vertical condensation. Scanning Electron Microscope Analysis: The specimens were longitudinally sectioned. Then samples were immersed in 17% EDTA solution for 10 minutes, immersed in 5.25 percent sodium hypochlorite for 10 minutes, and lastly washed entirely with water to remove and smear the layer. Carbon sputtering was performed in the middle and apical thirds of the root canal to aid SEM (500x magnification) evaluation.