scholarly journals Optimization of the Production of Lovastatin from Aspergillus sclerotiorum PSU- RSPG178 under Static Liquid Culture using Response Surface Methodology

2019 ◽  
Vol 48 (1) ◽  
pp. 93-102
Author(s):  
Sudarat Suwannarat ◽  
Jutarut Iewkittayakorn ◽  
Yaowapa Sukpondma ◽  
Vatcharin Rukachaisirikul ◽  
Souwalak Phongpaichit ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Liquid Culture ◽  
Aspergillus Sclerotiorum

Optimization of Liquid Culture Conditions of Methamidophos Degrading Enzyme from Staphylococcus rosehbach

2013 ◽  
Vol 303-306 ◽  
pp. 2683-2686 ◽  
Author(s):  
Na Zhang ◽  
Qing Qi Guo ◽  
Xiaofei Liu ◽  
Kun Song ◽  
Shuang Wang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Enzyme Activity ◽  
Response Surface ◽  
Liquid Culture ◽  
Soybean Protein ◽  
Culture Conditions ◽  
Box Behnken Design ◽  
Degrading Enzyme ◽  
Culture Time ◽  
Degradation Ratio

The nutritional parameters of methamidophos degrading enzyme, which is isolated from soil from Daqing and identified as Staphylococcus Rosehbach, were studied by response surface methodology (RSM). Glucose, peptone and culture time were analyzed by Box-Behnken design for optimizing their concentrations in the medium and the effect of their mutual interaction on methamidophos degrading enzyme activity. Glucose and soybean protein were found in influencing the methamidophos degrading enzyme activity highly significant. Glucose and soybean protein also showed an effect on the methamidophos degrading enzyme activity in interaction with other variables. The optima values of the parameters obtained through RSM, 0.31% glucose, 0.09% peptone and culture time 72h were applied. Actual value of verifiable experimentation was that the degradation ratio of methamidophos degrading enzyme to methamidophos was 39.8% at the optima conditions.

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