scholarly journals Soluble Expression of IFNα2-Tα1 Fusion Protein in Escherichia coli by N-terminal SUMO Fusion and its Anti-Proliferative Activity

2018 ◽  
Vol 50 (4) ◽  
Author(s):  
Muhammad Shahbaz Aslam ◽  
Iram Gull ◽  
Zaigham Abbas ◽  
Muhammad Amin Athar
Keyword(s):  
Escherichia Coli ◽  
Fusion Protein ◽  
Proliferative Activity ◽  
Soluble Expression ◽  
Sumo Fusion

Soluble Expression of a Human MnSOD and Hirudin Fusion Protein in Escherichia coli, and Its Effects on Metastasis and Invasion of 95-D Cells

2016 ◽  
Vol 26 (11) ◽  
pp. 1881-1890 ◽  
Author(s):  
Shanze Yi ◽  
Dewei Niu ◽  
Fang Bai ◽  
Shuaiguang Li ◽  
Luyuan Huang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fusion Protein ◽  
Soluble Expression ◽  
D Cells

High-level soluble expression of hIGF-1 fusion protein in recombinant Escherichia coli

2010 ◽  
Vol 45 (8) ◽  
pp. 1401-1405 ◽  
Author(s):  
Danping Zhang ◽  
Peilian Wei ◽  
Limei Fan ◽  
Jiazhang Lian ◽  
Lei Huang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fusion Protein ◽  
Soluble Expression ◽  
Recombinant Escherichia Coli ◽  
High Level

scholarly journals A fusion protein strategy for soluble expression of Stevia glycosyltransferase UGT76G1 in Escherichia coli

3 Biotech ◽  
2017 ◽  
Vol 7 (6) ◽  
Author(s):  
Liangliang Chen ◽  
Ping Sun ◽  
Yan Li ◽  
Ming Yan ◽  
Lin Xu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fusion Protein ◽  
Soluble Expression

scholarly journals Cloning and soluble expression of mature α-luffin from Luffa cylindrica inE. coli using SUMO fusion protein

2018 ◽  
Vol 42 ◽  
pp. 23-32 ◽  
Author(s):  
Shaghayegh NAMVAR ◽  
Farzaneh BARKHORDARI ◽  
Mozhgan RAIGANI ◽  
Hoda JAHANDAR ◽  
Leila NEMATOLLAHI ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Soluble Expression ◽  
Luffa Cylindrica ◽  
Sumo Fusion

Recombinant Nogo-66 via soluble expression with SUMO fusion in Escherichia coli inhibits neurite outgrowth in vitro

2015 ◽  
Vol 99 (14) ◽  
pp. 5997-6007 ◽  
Author(s):  
Xiaoyong Dai ◽  
Zhongqing Sun ◽  
Rui Liang ◽  
Yu Li ◽  
Huanmin Luo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Neurite Outgrowth ◽  
Soluble Expression ◽  
Sumo Fusion

scholarly journals Production of FMDV virus-like particles by a SUMO fusion protein approach in Escherichia coli

2009 ◽  
Vol 16 (1) ◽  
pp. 69 ◽  
Author(s):  
Chien-Der Lee ◽  
Yao-Pei Yan ◽  
Shu-Mei Liang ◽  
Ting-Fang Wang
Keyword(s):  
Escherichia Coli ◽  
Fusion Protein ◽  
Virus Like Particles ◽  
Sumo Fusion

Expression and partial purification of human pro-opiomelanocortin in Escherichia coli

1989 ◽  
Vol 3 (2) ◽  
pp. 105-112 ◽  
Author(s):  
T. S. Grewal ◽  
P. J. Lowry ◽  
D. Savva
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Fusion Protein ◽  
Western Blot ◽  
Blot Analysis ◽  
Expression Vectors ◽  
Partial Purification ◽  
Amino Acid Residues ◽  
E Coli ◽  
Dna Fragment

ABSTRACT A large portion of the human pro-opiomelanocortin (POMC) peptide corresponding to amino acid residues 59–241 has been cloned and expressed in Escherichia coli. A 1·0 kb DNA fragment encoding this peptide was cloned into the expression vectors pUC8 and pUR291. Plasmid pJMBG51 (a pUC8 recombinant) was found to direct the expression of a 24 kDa peptide. The recombinant pUR291 (pJMBG52) was shown to produce a β-galactosidase fusion protein of 140 kDa. Western blot analysis showed that both the 24 kDa and 140 kDa peptides are recognized by antibodies raised against POMC-derived peptides. The β-galactosidase fusion protein has been partially purified from crude E. coli cell lysates using affinity chromatography on p-aminobenzyl-1-thio-β-d-galactopyranoside agarose.

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