scholarly journals In Silico Prediction and Evaluation of E. coli Expressed Recombinant HA Protein of Avian Influenza Virus

2021 ◽  
Author(s):  
Sana Shakoor
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
In Silico ◽  
In Silico Prediction ◽  
E Coli ◽  
Ha Protein

scholarly journals Detection of avian influenza virus: a comparative study of the in silico and in vitro performances of current RT-qPCR assays

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Andrea Laconi ◽  
Andrea Fortin ◽  
Giulia Bedendo ◽  
Akihiro Shibata ◽  
Yoshihiro Sakoda ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Comparative Study ◽  
Avian Influenza Virus ◽  
In Silico ◽  
Influenza Virus A

scholarly journals H9N2 virus-derived M1 protein promotes H5N6 virus release in mammalian cells: Mechanism of avian influenza virus inter-species infection in humans

2021 ◽  
Vol 17 (12) ◽  
pp. e1010098
Author(s):  
Fangtao Li ◽  
Jiyu Liu ◽  
Jizhe Yang ◽  
Haoran Sun ◽  
Zhimin Jiang ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Mammalian Cells ◽  
Matrix Protein ◽  
H9n2 Virus ◽  
Virus Release ◽  
Mammalian Cell Lines ◽  
M1 Protein ◽  
Ha Protein

H5N6 highly pathogenic avian influenza virus (HPAIV) clade 2.3.4.4 not only exhibits unprecedented intercontinental spread in poultry, but can also cause serious infection in humans, posing a public health threat. Phylogenetic analyses show that 40% (8/20) of H5N6 viruses that infected humans carried H9N2 virus-derived internal genes. However, the precise contribution of H9N2 virus-derived internal genes to H5N6 virus infection in humans is unclear. Here, we report on the functional contribution of the H9N2 virus-derived matrix protein 1 (M1) to enhanced H5N6 virus replication capacity in mammalian cells. Unlike H5N1 virus-derived M1 protein, H9N2 virus-derived M1 protein showed high binding affinity for H5N6 hemagglutinin (HA) protein and increased viral progeny particle release in different mammalian cell lines. Human host factor, G protein subunit beta 1 (GNB1), exhibited strong binding to H9N2 virus-derived M1 protein to facilitate M1 transport to budding sites at the cell membrane. GNB1 knockdown inhibited the interaction between H9N2 virus-derived M1 and HA protein, and reduced influenza virus-like particles (VLPs) release. Our findings indicate that H9N2 virus-derived M1 protein promotes avian H5N6 influenza virus release from mammalian, in particular human cells, which could be a major viral factor for H5N6 virus cross-species infection.

Dual expression of the HA protein of H5N2 avian influenza virus in a baculovirus system

2006 ◽  
Vol 135 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Yu C. Hu ◽  
Yu L. Luo ◽  
Wen T. Ji ◽  
Julius L.C. Chulu ◽  
Poa C. Chang ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Baculovirus System ◽  
Ha Protein ◽  
Dual Expression

In-silico search of virus-specific host microRNAs regulating avian influenza virus NS1 expression

2015 ◽  
Vol 134 (1-2) ◽  
pp. 65-73 ◽  
Author(s):  
V. N. Muhasin Asaf ◽  
Amod Kumar ◽  
Ashwin Ashok Raut ◽  
Sandeep Bhatia ◽  
Anamika Mishra
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
In Silico ◽  
Specific Host ◽  
Ns1 Expression

scholarly journals Evaluation of the effect of simultaneous infection with E. coli O2 and H9N2 influenza virus on inflammatory factors in broiler chickens

2014 ◽  
Vol 4 (2) ◽  
Author(s):  
Habiballah Dadras ◽  
Saeed Nazifi ◽  
Marzieh Shakibainia
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Sialic Acids ◽  
Wing Vein ◽  
H9n2 Influenza Virus ◽  
E Coli ◽  
Amyloid A ◽  
Control Serum ◽  
Group 1

This study was conducted to evaluate the effect of experimental infection with <em>Escherichia</em> coli O2 and H9N2 influenza virus on inflammato- ry factors in broiler <em>chickens</em>. A total of 120 one-day-old Cobb broiler chicks were divided randomly to 6 groups. Inoculation program with 109 EID50/bird of the A/Chicken/Iran/772/1998 (H9N2) virus and 109 CFU/mL/bird of <em>E. coli</em> O2 was carried out as follows: the chicks in group 1 were inoculated with virus and bacteria simultaneously on day 26, group 2 received virus on day 26 and then bacteria 3 days later, group 3 were inoculated with bacteria on day 23 and then virus on day 26, group 4 received only bacteria on day 26, group 5 were inoculated with only virus on day 26 and group 6 served as control. Serum samples were collected from wing vein at days 20, 30, and 40. Sera were examined for inflammatory mediators (TNF-a and INF-γ), acute phase reactants (haptoglobin and serum amyloid A) and gangliosides (total, lipid-bound and protein-bound sialic acids) using validated standard procedures. Among the measured parameters, serum gangliosides showed significant differences between the challenged and control groups in different days post inoculation (P&lt;0.05). Significant increase in serum concentrations of serum sialic acids was observed on the 30th day in challenged groups. Elevations were found in the concentrations of serum gangliosides on day 40 compared to their first concentrations. The most obvious increase in serum sialic acids was observed in group 1 challenged with avian influenza virus and <em>E. coli</em> O2 simultaneously. Bacterial infected group showed more significant changes in comparison with viral infected one. These findings suggest that serum sialic acids may be a useful indicator of H9N2 avian influenza virus and avian pathogenic <em>E. coli</em> O2 co-infection.

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