BACK-TRANSMISSION OF APPLE STEM GROOVING VIRUS TO APPLE SEEDLINGS AND INDUCTION OF SYMPTOMS OF APPLE TOPWORKING DISEASE IN MITSUBA KAIDO (MALUS SIEBOLDII) AND KOBANO ZUMI (MALUS SIEBOLDII VAR. ARBORESCENS) ROOTSTOCKS

The reverse transcription polymerace chain reaction (RT-PCR) assay was successfully used for the detection of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in four apple cultivars of a 25 years old orchard. These two main pome fruit viruses were detected frequently in all tested apple cultivars. ASGV and ASPV occurred in as many as 16 trees (in the cultivar Spartan) and 13 trees (in the cultivar Idared) out of 20 tested trees, respectively. Mixed infection by ASGV and ASPV was found in all tested cultivars (as many as 9 out of 20 tested trees of the cultivar Spartan).

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Construction of full-length infectious cDNA clones of Apple stem grooving virus using Gibson Assembly method

Virus Research ◽

10.1016/j.virusres.2019.197790 ◽

2020 ◽

Vol 276 ◽

pp. 197790

Author(s):

Zheng-Nan Li ◽

Wilhelm Jelkmann ◽

Ping-ping Sun ◽

Lei Zhang

Keyword(s):

Full Length ◽

Cdna Clones ◽

Gibson Assembly ◽

Assembly Method ◽

Apple Stem Grooving Virus ◽

Apple Stem

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Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification

The Plant Pathology Journal ◽

10.5423/ppj.nt.06.2018.0108 ◽

2018 ◽

Vol 34 (6) ◽

pp. 575-579 ◽

Cited By ~ 1

Author(s):

Nam-Yeon Kim ◽

Jonghee Oh ◽

Su-Heon Lee ◽

Hongsup Kim ◽

Jae Sun Moon ◽

...

Keyword(s):

Reverse Transcription ◽

Recombinase Polymerase Amplification ◽

Specific Detection ◽

Apple Stem Grooving Virus ◽

Apple Stem

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Distribution of apple stem grooving virus and apple chlorotic leaf spot virus in infected in vitro pear shoots

Crop Protection ◽

10.1016/j.cropro.2010.08.003 ◽

2010 ◽

Vol 29 (12) ◽

pp. 1447-1451 ◽

Cited By ~ 17

Author(s):

L.P. Wang ◽

N. Hong ◽

G.P. Wang ◽

W.X. Xu ◽

R. Michelutti ◽

...

Keyword(s):

Leaf Spot ◽

Spot Virus ◽

Apple Stem Grooving Virus ◽

Apple Stem ◽

Chlorotic Leaf

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An Immunocapture RT-PCR Procedure Using Apple stem grooving virus Antibodies Facilitates Analysis of Citrus tatter leaf virus from the Original Meyer Lemon Host

Plant Disease ◽

10.1094/pdis-92-5-0746 ◽

2008 ◽

Vol 92 (5) ◽

pp. 746-750 ◽

Cited By ~ 6

Author(s):

Mark E. Hilf

Keyword(s):

Pcr Amplification ◽

Pairwise Alignment ◽

Magnetic Bead ◽

Genetic Distances ◽

Polyclonal Antiserum ◽

Apple Stem Grooving Virus ◽

Apple Stem ◽

Genomic Regions ◽

Leaf Virus ◽

Citrus Tatter Leaf Virus

A magnetic bead-based immunocapture system using polyclonal antiserum against Apple stem grooving virus (ASGV) successfully facilitated polymerase chain reaction (PCR) amplification of sequences from three Citrus tatter leaf virus (CTLV) isolates originally isolated from the citrus host Meyer lemon. Primers designed from a pairwise alignment of genomic sequences of CTLV isolates from lily and from kumquat amplified two nonoverlapping genomic regions of 625 and 1,165 bp (approximately 28% of the CTLV genome) which were cloned and sequenced. Despite being propagated separately in the glasshouse for more than 40 years, the CTLV sequences from separate Meyer lemon sources were identical but had only approximately 80% nucleotide identity with the homologous regions of CTLV genomes of isolates from lily and kumquat. Neighbor-joining phylogenetic analysis indicated the CTLV isolates from Meyer lemon were distinct from but more closely related to CTLV from kumquat than from lily, and these CTLV sequences showed equivalent genetic distances from two ASGV isolates.

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