DETECTION OF PLUM POX VIRUS USING A SIMPLIFIED POLYMERASE CHAIN REACTION PROCEDURE

1995 ◽  
pp. 383-390 ◽  
Author(s):  
L.M. Corvo ◽  
M. Sousa Santos ◽  
G. Nolasco
Keyword(s):  
Polymerase Chain Reaction ◽  
Plum Pox Virus ◽  
Chain Reaction ◽  
Polymerase Chain Reaction Procedure ◽  
Polymerase Chain

scholarly journals Resistance of Transgenic Prunus domestica to Plum Pox Virus Infection

Plant Disease ◽  
1997 ◽  
Vol 81 (11) ◽  
pp. 1231-1235 ◽  
Author(s):  
M. Ravelonandro ◽  
R. Scorza ◽  
J. C. Bachelier ◽  
G. Labonne ◽  
L. Levy ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Coat Protein ◽  
Plum Pox Virus ◽  
Prunus Domestica ◽  
Chain Reaction ◽  
Cp Gene ◽  
Aphid Feeding ◽  
Polymerase Chain ◽  
Dormancy Cycles ◽  
Das Elisa

Transgenic plum trees (Prunus domestica) containing the plum pox potyvirus coat protein (PPV-CP) gene were inoculated with PPV by aphid feeding or chip budding. Infection was monitored by evaluation of virus symptoms, DAS-ELISA, and immunoblot assays. Based on observations and analyses over 3 years including two dormancy cycles, one out of five transgenic clones (C-5), was found to be resistant to infection whether inoculated by aphids or by chip budding. PPV could not be detected in any inoculated plants of the C-5 clone by immunoblot or immunocap-ture-reverse transcriptase-polymerase chain reaction assays. To our knowledge, this is the first P. domestica clone resistant to PPV infection produced by genetic engineering.

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