The reaction of pear cultivars grafted on pear and quince rootstocks after artificial inoculation of Erwinia amylovora

2021 ◽  
pp. 321-328
Author(s):  
D. Aleksandrova ◽  
N. Dimitrova
Keyword(s):  
Erwinia Amylovora ◽  
Artificial Inoculation

scholarly journals Mapping of fire blight resistance in Malus ×robusta 5 flowers following artificial inoculation

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Andreas Peil ◽  
Christine Hübert ◽  
Annette Wensing ◽  
Mary Horner ◽  
Ofere Francis Emeriewen ◽  
...  
Keyword(s):  
Fire Blight ◽  
Erwinia Amylovora ◽  
Interval Mapping ◽  
Artificial Inoculation ◽  
Blight Resistance ◽  
Bacterial Disease ◽  
Phenotypic Data ◽  
Resistance Qtl ◽  
Common Path ◽  
Major Qtl

Abstract Background Although the most common path of infection for fire blight, a severe bacterial disease on apple, is via host plant flowers, quantitative trait loci (QTLs) for fire blight resistance to date have exclusively been mapped following shoot inoculation. It is not known whether the same mechanism underlies flower and shoot resistance. Results We report the detection of a fire blight resistance QTL following independent artificial inoculation of flowers and shoots on two F1 segregating populations derived from crossing resistant Malus ×robusta 5 (Mr5) with susceptible ‘Idared’ and ‘Royal Gala’ in experimental orchards in Germany and New Zealand, respectively. QTL mapping of phenotypic datasets from artificial flower inoculation of the ‘Idared’ × Mr5 population with Erwinia amylovora over several years, and of the ‘Royal Gala’ × Mr5 population in a single year, revealed a single major QTL controlling floral fire blight resistance on linkage group 3 (LG3) of Mr5. This QTL corresponds to the QTL on LG3 reported previously for the ‘Idared’ × Mr5 and an ‘M9’ × Mr5 population following shoot inoculation in the glasshouse. Interval mapping of phenotypic data from shoot inoculations of subsets from both flower resistance populations re-confirmed that the resistance QTL is in the same position on LG3 of Mr5 as that for flower inoculation. These results provide strong evidence that fire blight resistance in Mr5 is controlled by a major QTL on LG3, independently of the mode of infection, rootstock and environment. Conclusions This study demonstrates for the first time that resistance to fire blight caused by Erwinia amylovora is independent of the mode of inoculation at least in Malus ×robusta 5.

scholarly journals Response of Central European Pyrus Germplasm to Natural Fire Blight Infection and Artificial Inoculation

HortScience ◽  
1995 ◽  
Vol 30 (6) ◽  
pp. 1287-1291 ◽  
Author(s):  
Tom van der Zwet ◽  
Richard L. Bell
Keyword(s):  
Central Europe ◽  
Fire Blight ◽  
Erwinia Amylovora ◽  
Former Yugoslavia ◽  
Artificial Inoculation ◽  
Research Station ◽  
Natural Fire ◽  
Central European ◽  
Moderately Resistant

Of 133 Pyrus accessions (predominantly P. communis L.), collected in Central Europe and previously rated in the resistant U.S. Dept. of Agriculture (USDA) blight scores 10–6, only 77 (57.0%) remained in these scores after an additional 5 years of exposure to fire blight [Erwinia amylovora (Burr.) Winsl. et al.]. Of these, 24 originated from three states in former Yugoslavia. Following several years of severe blight epiphytotics, only 5 (10.4%) of 52 accessions released from quarantine since 1986 and planted at Appalachian Fruit Research Station scored 6 or above. All accessions were highly susceptible to artificial blossom inoculation, and only 10 accessions were at least moderately resistant to artificial shoot inoculations.

Application of commercial test-systems to identify gram-negative facultatively anaerobic bacteria

2016 ◽  
Vol 3 (1) ◽  
pp. 43-48 ◽  
Author(s):  
V. Patyka ◽  
L. Butsenko ◽  
L. Pasichnyk
Keyword(s):  
Erwinia Amylovora ◽  
Anaerobic Bacteria ◽  
Biochemical Properties ◽  
Test System ◽  
Phytopathogenic Bacteria ◽  
Gram Negative ◽  
Test Systems ◽  
Facultatively Anaerobic ◽  
Microbiological Methods

Aim. To validate the suitability of commercial API 20E test-system (bioMerieux) for the identifi cation and characterization of facultative gram-negative phytopathogenic bacterial isolates. Methods. Conventional mi- crobiological methods, API 20E test-system (bioMerieux) according to the manufacturer’s instructions. Re- sults. The identifi cation results for Erwinia amylovora, Pectobacterium carotovorum and Pantoea agglome- rans isolates were derived from the conventional and API 20E test systems, which, were in line with the literature data for these species. The API 20E test-system showed high suitability for P. agglomerans isolates identifi cation. Although not all the species of facultatively anaerobic phytopathogenic bacteria may be identi- fi ed using API 20E test-system, its application will surely allow obtaining reliable data about their physiologi- cal and biochemical properties, valuable for identifi cation of bacteria, in the course of 24 h. Conclusions. The results of tests, obtained for investigated species while using API 20E test-system, and those of conventional microbiological methods coincided. The application of API 20E test-system (bioMerieux) ensures fast obtain- ing of important data, which may be used to identify phytopathogenic bacteria of Erwinia, Pectobacterium, Pantoea genera.

Seasonal wound presence and susceptibility to Neonectria ditissima infection in New Zealand apple trees

2015 ◽  
Vol 68 ◽  
pp. 250-256 ◽  
Author(s):  
N.T. Amponsah ◽  
M. Walter R.M. Beresford ◽  
R.W.A. Scheper
Keyword(s):  
New Zealand ◽  
Artificial Inoculation ◽  
Early Winter ◽  
Leaf Fall ◽  
Apple Orchards ◽  
Apple Trees ◽  
Leaf Scar ◽  
Fruit Thinning ◽  
Different Types

Leaf scar wounds are important sites for Neonectria ditissima infection of apple trees Monitoring leaf fall in Scilate/Envy and Braeburn trees to estimate leaf scar wound presence showed maximum leaf scar incidence occurred in June (early winter) Wounds detected in New Zealand apple orchards were bud scale scars fruit thinning and picking wounds leaf scars and pruning cuts Picking wounds are caused during harvest where the pedicel is detached from the shoot Susceptibility of these different types of wounds was determined using artificial inoculation of N ditissima conidia during the season Pruning cut wounds were the most susceptible followed by fruit picking and thinning wounds and the least susceptible were leaf scar wounds No infections were observed when bud scale wounds were inoculated There was no difference in wound susceptibility between cultivars but overall Scilate/Envy wounds developed more lesions than Braeburn wounds

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