Nomenclature standards and DNA barcoding of apple varieties originated by VIR Crimean Experimental Breeding Station

Current pace of breeding technologies relies on a tighter control of quality and identity of breeding achievements. Thereby, standardised variety nomenclature becomes of particular relevance to store original genetic information and warrant authenticity of the breeding product. A nomenclature standard regulates the variety’s name and habitus. A herbarium specimen is most appropriate for standardisation, since it defines a set of strongly inherited morphological characters for a variety. Nomenclature standards must be permanently stored in a scientific herbarium collection. The N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) in alliance with national plant breeders initiated a first nomenclature standard collection of national-breeding cultivars. Aside to classical herbarising, the collection utilises molecular genetic techniques, particularly genotyping, to enable extended variety verification. The VIR team has developed the nomenclature standards of six apple varieties originated by the Crimean Experimental Breeding Station, a VIR Branch (Beloe Solntse, Zolotoy Potok, Kubanochka, Leto Krasnoe, Luchistoe and Shchedrost). The standards are supplemented with DNA barcodes obtained jointly with the N. I. Vavilov Institute of General Genetics. Fifteen microsatellite markers were used for barcoding. The herbarium nomenclature specimens adhered to the VIR-developed protocol in accordance with the International Code of Nomenclature for Cultivated Plants (ICNCP). The specimens are registered in the VIR Herbarium database and deposited in the World Crop Wild and Weed Relatives (WIR) Herbarium type collection.

Phenolics and Mineral Elements Composition in Underutilized Apple Varieties

(1) Background: The aim of this research is to study the importance of improving knowledge of old variety apples in terms of phytochemicals, antioxidant capacity and mineral elements. (2) Methods: Fifteen phenolic compounds (UHPLC-MS/MS techniques), eight mineral elements (Ca, K, Cu, Mn, Fe, Zn, Na and Mg), antioxidant capacity (DPPH%) and vitamin C content in four ancient apple varieties of Tuscany (‘Mora’, ‘Nesta’, ‘Panaia’ and ‘Ruggina’), with the ‘Golden Delicious’ cultivar as reference, were analyzed. (3) Results: Ancient cultivars exhibited a superior antioxidant capacity compared to commercial cultivars. ECTC and CGA were the polyphenols that mostly characterized the ancient apples. The contents of the elements of concern, Zn and Mn, were higher in the ancient cultivars ‘Panaia’ and ‘Ruggina’, while Na showed significant low concentration in ‘Nesta’, ‘Panaia’ and ‘Ruggina’ compared to ‘Golden Delicious’. The vitamin C content also indicated that ‘Mora’, ‘Panaia’ and ‘Ruggina’ old variety had an AsA content around 10 times higher than the ‘Golden Delicious’ apple. (4) Conclusions: Underutilized varieties could be an excellent source of bioactive phenolic phytochemicals, mineral nutrients and vitamins that may offer special nutraceutical benefits compared to other fruits.

BBAP amplification profiles of apple varieties

Several types of allergies are currently known and are characterized by an exaggerated response of the immune system to substances from various sources called allergens. One of them is a food allergy, which is becoming more common in the population. For this reason, it is necessary to describe the issue from several aspects including genomic variability of plant allergens. The objective of this study was to analyse intraspecific variability of Bet v 1 of 10 different varieties of apple species (Malus domestica Borkh.). BBAP technique for genomic determination of the presence of Bet v 1 homologs at the DNA level was performed. Degenerate primers that anneal a variable and conserved part of PR-10 protein homologues genes were used in the analyse. Amplicons were generated and formed relatively monomorphic profiles, indicating the stability of the given isoforms of Bet v 1 within the selected apple varieties. To evaluate the potential allergenicity of selected varieties further studies on another molecular level such as a comparison of gene expression of the PR-10 family members and their protein expression levels are needed.

Growth features of apple varieties and forms with natural and compact crown types

This research explores the compactness of apple varieties and forms with different types of crown. Orchards on semi-vigorous rootstocks with density and high density planting systems are cost-effective in modern horticulture. For making such gardens, it is necessary to have trees with special compact crown shape. They ensure the high-quality fruits production, meeting the requirements of world standards. To date the most perspective apple varieties and forms for domestic intensive gardening from the rich gene pool of the Selection and Genetic Center of Federal State Scientific Institution «I. V. Michurin Federal Scientific Center» are identified. Varieties of folk domestic and foreign breeding took part in the creation of new genotypes. Apple varieties and promising forms (more than 160 genotypes), including from near and far abroad selection, were used as materials for crown features studying. We conducted our study by the following indicators: tree height, crown volume, “degree of compactness” and the shoot-forming ability. We found that the genotypes Sholokhovskoye, Zvezda Artemyeva, 25-7(11), 40-9(8), 36-8(21), 40-9(6), 8-7(72), 40-9(7), 36-8(17), 62-5(140) have optimal crown parameters. We recommend their use for industrial horticulture and further breeding to create restrained growth, low-volume crown, and a high degree of compactness varieties.

Assessment of economically valuable traits of apple varieties and features of the main elements of the technology for their cultivation in an arid climate

The purpose of the research is to create winter-hardy, large-fruited, high-yielding, high-quality varieties of apple trees of different ripening periods and their growing technology (use of a biological product) in the South Urals. The research was carried out on the basis of Orenburg Experimental Station of Horticulture and Viticulture - a branch of the Federal State Budgetary Scientific Institution “Federal Scientific Selection and Technology Center for Horticulture and Nursery ” from 1999 to 2020. Research objects were summer apple varieties – “Orenburgskoe”, “Orenburgskoe krasnoe”, “Solntsedar” (K); winter – “Orenburgskoe pozdnee”, “Zimnee”, “Yuzhnouralskoe” (K). The counts and observations were carried out according to generally accepted methods. As a result of the studies, the data obtained showed that the property of short stature as a genetic trait of resistance is transmitted to the offspring during hybridization. The varieties “Orenburgskoe pozdnee”, “Zimnee” and “Yuzhnouralskoe” showed the greatest increase in productivity embodiment experience “Evrikor Forte + 7” 2.5 l / ha by 25.4%, 24.3% and 22.2% respectively. Thus, the application of “Eurikor-Forte + 7” increased both the number of fruits and their weight, and also increased the yield of the studied varieties.

Bacterial Endophyte Community Dynamics in Apple (Malus domestica Borkh.) Germplasm and Their Evaluation for Scab Management Strategies

The large genetic evolution due to the sexual reproduction-mediated gene assortments and propensities has made Venturia inaequalis (causing apple scab) unique with respect to its management strategies. The resistance in apple germplasm against the scab, being controlled for by more than fifteen genes, has limited gene alteration-based investigations. Therefore, a biological approach of bacterial endophyte community dynamics was envisioned across the apple germplasm in context to the fungistatic behavior against V. inaequalis. A total of 155 colonies of bacterial endophytes were isolated from various plant parts of the apple, comprising 19 varieties, and after screening for antifungal behavior followed by morphological, ARDRA, and sequence analysis, a total of 71 isolates were selected for this study. The alpha diversity indices were seen to fluctuate greatly among the isolation samples in context to microflora with antifungal behavior. As all the isolates were screened for the presence of various metabolites and some relevant genes that directly or indirectly influence the fungistatic behavior of the isolated microflora, a huge variation among the isolated microflora was observed. The outstanding isolates showing highest percentage growth inhibition of V. inaequalis were exploited to raise a bio-formulation, which was tested against the scab prevalence in eight apple varieties under controlled growth conditions. The formulation at all the concentrations caused considerable reductions in both the disease severity and disease incidence in all the tested apple varieties. Red Delicious being most important cultivar of the northwestern Himalayas was further investigated for its biochemical behavior in formulation and the investigation revealed different levels of enzyme production, chlorophyll, and sugars against the non-inoculated control.