Wieland. Serodiagnosis of guinea pig serum tuberculosis and its significance for the experimental study of tuberculosis (Ztschr. F. Immunitätsf .. Bd 88, H. 1/2 193)

1937 ◽  
Vol 33 (5) ◽  
pp. 635-635
Keyword(s):  
Experimental Study ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
General Significance ◽  
Pig Serum

The author tried to resolve two questions: firstly, which of the serological reactions is most suitable for detecting changes in the blood of tuberculous guinea pigs, and, secondly, what is the general significance of serodiagnostics in this animal with tuberculosis.

scholarly journals EVIDENCE THAT THE L-ASPARAGINASE OF GUINEA PIG SERUM IS RESPONSIBLE FOR ITS ANTILYMPHOMA EFFECTS

1963 ◽  
Vol 118 (1) ◽  
pp. 99-120 ◽  
Author(s):  
J. D. Broome
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Serum Proteins ◽  
Deae Cellulose ◽  
Salt Precipitation ◽  
Asparaginase Activity ◽  
Pig Serum

A number of the properties of the L-asparaginase present in guinea pig serum have been examined and shown to be indistinguishable from those of the agent responsible for inhibiting cells of lymphoma 6C3HED in vivo. The patterns of instability of the enzyme to changes in temperature and pH were found to parallel closely those of the antilymphoma agent. L-Asparaginase activity was essentially absent from the serum of newborn guinea pigs and this failed to inhibit 6C3HED cells. On separating guinea pig serum proteins by salt precipitation, electrophoresis, and chromatography on DEAE cellulose, antilymphoma activity was found only in fractions which contained L-asparaginase.

scholarly journals FURTHER STUDIES ON TYPHUS FEVER

1936 ◽  
Vol 64 (5) ◽  
pp. 673-687 ◽  
Author(s):  
Hans Zinsser ◽  
Attilio Macchiavello
Keyword(s):  
Tissue Culture ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Horse Serum ◽  
Tissue Cultures ◽  
Practical Application ◽  
Typhus Fever ◽  
Culture Virus ◽  
Pig Serum

1. Guinea pigs can be actively immunized against European typhus fever with homologous formalinized Rickettsia tissue cultures, provided sufficient amounts are injected. The method is suggested for practical application in man. 2. Serovaccination against European typhus fever can be successfully applied to guinea pigs by a variety of methods, the simplest of which consists of the injection of mixtures of virulent defibrinated guinea pig blood and convalescent guinea pig serum taken from 3 to 5 days after defervescence. Similar results can be obtained with mixtures in which tissue culture virus, either with convalescent guinea pig serum or with antimurine horse serum, is used. There is no indication so far that such animals become carriers. Possible application of these methods to typhus epidemics is discussed.

scholarly journals THE RÔLE OF THE RETICULO-ENDOTHELIAL SYSTEM IN IMMUNITY

1926 ◽  
Vol 43 (6) ◽  
pp. 797-806 ◽  
Author(s):  
C. W. Jungeblut ◽  
J. A. Berlot
Keyword(s):  
Methylene Blue ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Full Extent ◽  
Intravenous Injections ◽  
India Ink ◽  
Normal Functional ◽  
Reticulo Endothelial System ◽  
Pig Serum

1. Intravenous injections of India ink into guinea pigs caused a decided drop in the complement titer which set in as early as 15 minutes after the injection, but did not reach its maximum for 3 hours. This drop was followed by a return to normal within the first 24 hours following the injection. 2. India ink mixed in vitro with guinea pig serum adsorbs the complement almost immediately to its full extent. 3. By means of reduction tests (methylene blue and nitroanthraquinone) it was shown that the respiration of the cells of the liver and spleen of guinea pigs was markedly impaired for the first 8 hours, following an intravenous injection of ink. Evidences of a return to normal functional vitality, however, became apparent by the end of the 1st day after the injection.

scholarly journals THE RELATION BETWEEN ANTIANAPHYLAXIS AND ANTIBODY BALANCE

1936 ◽  
Vol 64 (4) ◽  
pp. 641-655 ◽  
Author(s):  
Marion C. Morris
Keyword(s):  
Guinea Pig ◽  
Intravenous Injection ◽  
Anaphylactic Reaction ◽  
Guinea Pigs ◽  
Normal Serum ◽  
Intravenous Inoculation ◽  
Lethal Doses ◽  
Circulating Antibody ◽  
Pig Serum ◽  
Increased Susceptibility

1. Sensitized guinea pigs injected with normal rabbit or guinea pig serum previous to intravenous inoculation of antigen may be protected against a few lethal doses of antigen. The protection is greater with foreign than with homologous serum and appears to be related roughly to the amount of serum introduced. 2. Sensitized guinea pigs injected with antibody-containing serum preliminary to intravenous injection of antigen, show no greater refractoriness to anaphylaxis than do those injected with normal serum. 3. Moreover, in many instances, the injection of an excess of antibody into the circulation of sensitized guinea pigs, leads to an increased susceptibility of these animals to anaphylaxis. 4. These results indicate that an excess of circulating antibody is not responsible for a state of antianaphylaxis, but on the contrary, may contribute toward the anaphylactic reaction itself.

scholarly journals OBSERVATIONS ON THE RELEASE OF SERUM FIBRINOLYSIN BY SPECIFIC ANTIGEN, PEPTONE, AND CERTAIN POLYSACCHARIDES

1949 ◽  
Vol 90 (1) ◽  
pp. 39-51 ◽  
Author(s):  
Georges Ungar ◽  
Shirley H. Mist
Keyword(s):  
Hyaluronic Acid ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Specific Antigen ◽  
Anaphylactoid Reactions ◽  
Pneumococcal Polysaccharides ◽  
Pig Serum

Formation of fibrinolysin from its inactive precursor in serum was observed under the following conditions: (a) by adding the specific antigen to serum from sensitized guinea pigs; (b) by mixing normal guinea pig serum with peptone, agar, hyaluronic acid, chondroitinsulfuric acid, glycogen, pneumococcal polysaccharides, and heparin. Activation of profibrinolysin by these agents differs from chloroform or streptokinase activation in that it requires the presence of some serum constituent non-precipitable with the euglobulin fraction and destroyed by heating at 56°C. The bearing of these observations on the mechanism of anaphylactic and anaphylactoid reactions is discussed. The findings reported support the concept that proteolysis is part of the process determining the release of histamine and other toxic products. It is suggested that the presence of fibrinokinase may be responsible for the toxicity of serum induced in vitro by a number of agents.

scholarly journals Some observations on reversed anaphylaxis

1939 ◽  
Vol 39 (5) ◽  
pp. 471-497 ◽  
Author(s):  
M. van den Ende
Keyword(s):  
Guinea Pig ◽  
Intravenous Injection ◽  
Guinea Pigs ◽  
Immune Reaction ◽  
Serum Proteins ◽  
Systemic Blood Pressure ◽  
Serum Fractions ◽  
Bronchial Resistance ◽  
Pulmonary Arterial ◽  
Pig Serum

1. The symptoms and autopsy findings in guinea-pigs following intravenous injection of antisera prepared against guinea-pig serum or serum fractions are described. Two types of reaction were observed, acute and delayed, similar to those described in direct anaphylaxis.2. The alterations in systemic blood pressure, pulmonary arterial pressure, and bronchial resistance, were investigated and found to simulate closely those observable in ordinary anaphylactic shock.3. The antisera have the power of stimulating contraction of the isolated uterus of the guinea-pig, either in the presence or absence of excess guinea-pig serum. The reaction, like that observed in direct anaphylaxis, is therefore cellular.4. Antisera prepared against guinea-pig serum proteins contain, in addition to precipitins, agglutinins for the red cells of that species, and Forssmann antibody. Neither of the last two antibodies, however, is responsible for the shock phenomena here described. It appears that the potency of a serum to produce shock in guinea-pigs is dependent on several factors, of which the most important is the content in precipitins reacting with the guinea-pig serum proteins. These precipitins give rise to the reactions following intravenous injection into guinea-pigs, not merely as a result of humoral combination with homologous antigens, but largely, if not wholly, as the result of an immune reaction with antigens in the protoplasm of the tissue cells.

AN EXPERIMENTAL STUDY OF POST-PARTUM ENDOMETRIAL REPAIR IN THE GUINEA-PIG AND RAT

1952 ◽  
Vol 8 (2) ◽  
pp. 162-NP
Author(s):  
A. SHARMAN
Keyword(s):  
Experimental Study ◽  
Guinea Pig ◽  
Corpus Luteum ◽  
Guinea Pigs ◽  
Post Partum ◽  
The Third ◽  
Endometrial Regeneration

An experimental study was made of post-partum endometrial regeneration in twenty-one healthy guinea-pigs and in twenty healthy rats. To serve as controls, one guinea-pig was killed on the day of parturition and another 6½ days following parturition: three rats were killed on the day of parturition and one 6½ days following parturition. All the remaining animals were ovariectomized. Groups of three were killed at the following intervals after parturition, viz.: 22, 40, 58, 84 hr. and 6½ days. In each group, one animal was left uninjected, another injected with oestrogenic hormone and the third injected with corpus luteum hormone. Endometrial regeneration appeared to proceed normally in all the animals.

scholarly journals Heat labile and heat stable factors and antibodies in normal and immune sera against T-system bacteriophages

1960 ◽  
Vol 58 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Enver Tall Çetin
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Heat Stable ◽  
Heat Labile ◽  
Pig Serum ◽  
T System

1. Heat labile and heat stable inactivating factors against T-system phages are present in normal guinea-pig and rabbit sera. These may be antibodies.2. Sera of guinea-pigs immunized with phages T2, T3 and T4 contain heat labile and heat stable antibodies.3. The heat labile activity is absorbed from guinea-pig serum by phage more readily than the heat stable activity.4. Dilution of the sera reduces the heat labile activity more than the heat stable activity.5. Complement has no effect on the inactivation or neutralization of phages T2, T3 and T4 by normal or immune guinea-pig sera.

scholarly journals PROPERTIES OF GUINEA PIG 7S ANTIBODIES

1963 ◽  
Vol 117 (6) ◽  
pp. 937-949 ◽  
Author(s):  
Baruj Benacerraf ◽  
Zoltan Ovary ◽  
Kurt J. Bloch ◽  
Edward C. Franklin
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Analytical Ultracentrifugation ◽  
Antigenic Determinants ◽  
Protein Antigens ◽  
Intraperitoneal Route ◽  
Electrophoretic Mobilities ◽  
Single Protein ◽  
Pig Serum

Guinea pigs hyperimmunized with single protein antigens or hapten conjugates emulsified in complete adjuvants produced two types of precipitating antibodies with different electrophoretic mobilities. "Slow" migrating antibody generally appeared earlier and "fast" migrating antibody later in the course of immunization. Animals initially immunized by the intraperitoneal route with hapten conjugates without adjuvants produced primarily fast migrating antibody. Purified guinea pig antibodies were also separable into slow and fast migrating components by electrophoresis in supporting media. Using suitable antisera prepared in rabbits hyperimmunized with guinea pig serum, it was demonstrated that slow and fast antibodies have both common and distinct antigenic determinants. Analytical ultracentrifugation disclosed that both antibodies have sedimentation coefficients of approximately 7S. These antibodies have been designated guinea pig 7Sγ1 and 7Sγ2.

Effect of Psoraleae Gel on Vitiligo Animal Pathological Pattern

2013 ◽  
Vol 850-851 ◽  
pp. 1263-1266
Author(s):  
Ming San Miao ◽  
Dan Dan Liu ◽  
Jing Yi Qiao
Keyword(s):  
Animal Models ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Low Dose ◽  
Pathological Changes ◽  
Model Group ◽  
Moderate Dose ◽  
Pig Skin ◽  
Injection Methods ◽  
Pig Serum

Objective: Explore the effect of psoraleae gels external use on vitiligo guinea pig, to prove psoraleae gel can promote the formation of melanin guinea pig skin like psoraleae injection. Methods: Using chemical decolorization to establish vitiligo animal models, treatment by psoraleae gel, observation the effect of high, moderate and low dose of psoraleae gel external use in vitiligo guinea pig serum ChE activity and MDA level, skin pathological changes and the sum total of skin melanin granules. Results: Compared with model group, high, moderate dose psoraleae gel group can significantly improve the serum ChE activity, on vitiligo mode, High, moderate dose psoraleae gel group can significantly improve the serum ChE activity and significantly reduce serum MDA level, high, moderate and low dose psoraleae gel group were significantly promote the guinea pig skin melanin formation and the sum total of melanin granules increased significantly. Conclusion: Psoraleae gel treatments of vitiligo guinea pigs have a very good role; this experiment can show psoraleae gel can be used in the clinical treatment of vitiligo.

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