scholarly journals Difficulties of life-time diagnosis of rabies. Clinical observation

2018 ◽  
Vol 35 (4) ◽  
pp. 88-93
Author(s):  
A. Kh. Mamunts ◽  
G. V. Batrakova ◽  
I. N. Trefilov ◽  
N. I. Ogorodova ◽  
M. A. Mamunts
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Observation ◽  
Clinical Symptoms ◽  
Life Time ◽  
Autopsy Material ◽  
Chain Reaction ◽  
Lethal Outcome ◽  
Virus Rna ◽  
Polymerase Chain ◽  
Time Diagnosis

The paper presents a case of rabies with lethal outcome in a fourteen-year-old adolescent. The child did not undergo postexposure prevention of rabies after a cat’s attack in connection with late addressing for medical care, just after occurrence of clinical symptoms of this disease. The child was not diagnosed “rabies” when was alive. Diagnosis was verified by detection of rabies virus RNA in autopsy material using polymerase chain reaction.

scholarly journals Peripheral Blood Leukocytes and Serum Nested Polymerase Chain Reaction Are Complementary Methods for Monitoring Active Cytomegalovirus Infection in Transplant Patients

2013 ◽  
Vol 24 (3) ◽  
pp. e69-e74 ◽  
Author(s):  
PD Andrade ◽  
MT Fioravanti ◽  
EBV Anjos ◽  
C De Oliveira ◽  
DM Albuquerque ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Positive Predictive Value ◽  
Predictive Value ◽  
Cytomegalovirus Infection ◽  
Clinical Symptoms ◽  
Peripheral Blood Leukocytes ◽  
Active Infection ◽  
Chain Reaction ◽  
Blood Leukocytes ◽  
Polymerase Chain

BACKGROUND: Human cytomegalovirus is an important cause of morbidity and mortality in immunocompromised patients. Qualitative polymerase chain reaction (PCR) has proven to be a sensitive and effective technique in defining active cytomegalovirus infection, in addition to having low cost and being a useful test for situations in which there is no need for quantification. Real-time PCR has the advantage of quantification; however, the high cost of this methodology makes it impractical for routine use.OBJECTIVE: To apply a nested PCR assay to serum (sPCR) and to evaluate its efficiency to diagnose active cytomegalovirus infection compared with PCR of peripheral blood leukocytes (L-PCR).METHODS: Samples of 37 patients were prospectively evaluated. An internal control was created and applied to sPCR to exclude false-negative results.RESULTS: In total, 21 patients (57%) developed active cytomegalovirus infection. After analyzing the two methods for the diagnosis of active infection, higher sensitivity and negative predictive value of the L-PCR versus sPCR (100% versus 62%), and higher specificity and positive predictive value of sPCR versus L-PCR (81% versus 50% and 72%, respectively) were observed. Discordant results were observed in 11 patients who were L-PCR-positive but sPCR-negative for active cytomegalovirus infection, five of whom developed clinical symptoms of cytomegalovirus. Clinical symptoms were observed in 14 patients, 12 of whom were diagnosed with active infection by nested L-PCR (P=0.007) and seven by nested sPCR (P=0.02). Higher specificity and a positive predictive value for sPCR were observed.CONCLUSION: Nested L-PCR and sPCR were considered to be complementary methods for the diagnosis and management of symptomatic cytomegalovirus infection.

scholarly journals Overestimation and underestimation of hepatitis C virus RNA levels in a widely used real-time polymerase chain reaction-based method

Hepatology ◽  
2007 ◽  
Vol 46 (1) ◽  
pp. 22-31 ◽  
Author(s):  
Stéphane Chevaliez ◽  
Magali Bouvier-Alias ◽  
Rozenn Brillet ◽  
Jean-Michel Pawlotsky
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Real Time ◽  
Hepatitis C Virus Rna ◽  
Chain Reaction ◽  
Virus Rna ◽  
Polymerase Chain ◽  
Rna Levels
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