scholarly journals Interaction of rifampicin embedded in phospholipid nanoparticles with blood plasma lipoproteins

2014 ◽  
Vol 60 (3) ◽  
pp. 348-353 ◽  
Author(s):  
M.A. Sanzhakov ◽  
O.M. Ipatova ◽  
V.N. Prozorovskiy ◽  
N.V. Medvedeva ◽  
T.I. Torkhovskaya
Keyword(s):  
Blood Plasma ◽  
Sodium Oleate ◽  
Protein Fraction ◽  
Plasma Lipoproteins ◽  
Ldl Receptors ◽  
Phospholipid Nanoparticles ◽  
Plasma Fractions ◽  
Plasma Protein Fraction ◽  
Blood Plasma Lipoproteins ◽  
Biomedical Chemistry

The drug formulations of antituberculous remedy rifampicin in nanoparticles less than 30 nm based on soy phosphatidylcholine and sodium oleate was elaborated in Institute of Biomedical Chemistry. The distribution of rifampicin in blood plasma fractions after incubation with this formulation and with free rifampicin was studied. This goal was stimulated by the literature data about activation of macrophages LDL receptors in cases of M. tuberculosis infection. Plasma was incubated 30 min with free rifampicin or rifampicin encapsulated into the nanoformulation followed by ultracentrifugation and subsequent rifampicin determination by HPLC in lipoprotein fractions. In the case of free rifampicin it appeared mainly in the plasma protein fraction and in HDL (41% and 38%, correspondentely). But after incubation of rifampicin in nanoparticles the drug redistribution was observed. Its proportion in these factions decreased 2-3-fold, and it was found mainly in LDL (60% as compared with 21% for free rifampicin). The increased association of rifampicin encapsulated into phospholipid nanoparticles with LDL is considered as facilitating factor for macrophages delivery and thus for antituberculosis efficiency as well

scholarly journals IMMUNOCHEMICAL STUDIES ON HUMAN PLASMA LIPOPROTEINS

1957 ◽  
Vol 105 (1) ◽  
pp. 49-67 ◽  
Author(s):  
Frederick Aladjem ◽  
Miriam Lieberman ◽  
John W. Gofman
Keyword(s):  
Human Plasma ◽  
Plasma Protein ◽  
Protein Fraction ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Plasma Lipoproteins ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Plasma Protein Fraction ◽  
Free Plasma

Low density human plasma lipoproteins Sf 17+, Sf 13, and Sf 6, high density lipoproteins 2 and 3, and a lipoprotein-free plasma protein fraction were isolated from human plasma by ultracentrifugal methods. It was found that human plasma lipoproteins are immunochemically distinct from the non-lipoprotein containing plasma protein fraction. Lipoprotein fractions of a given hydrated density, isolated from different individuals, were found to be immunochemically indistinguishable by qualitative absorption tests. Qualitative antigenic differences were shown to exist between low density lipoproteins and high density lipoproteins. Quantitative precipitin reactions showed that low density lipoproteins Sf 6 and Sf 13 were immunochemically very similar. However, they differed with respect to the amount of antigen nitrogen required for maximum precipitation. Agar diffusion analyses were performed; the results suggest heterogeneity of lipoproteins by this criterion.

Comparison of Methods for separating Blood Plasma Lipoproteins

Nature ◽  
1965 ◽  
Vol 206 (4980) ◽  
pp. 191-192 ◽  
Author(s):  
G. L. MILLS ◽  
C. E. TAYLAUR ◽  
PATRICIA A. WILKINSON
Keyword(s):  
Blood Plasma ◽  
Plasma Lipoproteins ◽  
Comparison Of Methods ◽  
Blood Plasma Lipoproteins

The Chemiluminescence Assay of Lipid Peroxidation Products in Human Blood Plasma Lipoproteins

1994 ◽  
Vol 20 (5) ◽  
pp. 279-288 ◽  
Author(s):  
Elena Driomina ◽  
Igor Polnikov ◽  
Victor Sharov ◽  
Ofelia Azizova ◽  
Yury Vladimirov
Keyword(s):  
Lipid Peroxidation ◽  
Blood Plasma ◽  
Human Blood ◽  
Plasma Lipoproteins ◽  
Human Blood Plasma ◽  
Lipid Peroxidation Products ◽  
Chemiluminescence Assay ◽  
Blood Plasma Lipoproteins
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