scholarly journals Vegetative propagation of freesia through callus cultures.

1974 ◽  
Vol 22 (3) ◽  
pp. 153-159
Author(s):  
Y.P.S. Bajaj ◽  
R.L.M. Pierik
Keyword(s):  
Callus Induction ◽  
Vegetative Propagation ◽  
Callus Cultures ◽  
Free Medium ◽  
Flower Buds ◽  
Flower Bud ◽  
Bud Formation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Stem Leaf

Isolated segments of corm, stem, leaf, flower bud and anther from flowering freesia plants, cv. Ballerina, were induced to form callus on a modified Murashige and Skoog medium supplemented with NAA and 6-(benzylamino)-9-(2-tetrahydropyranyl)-9H-purine (PBA). Callus induction was best with young flower buds kept at 25 deg C in darkness. Callus could be sub-cultured in darkness on a medium containing auxin and cytokinin. Complete plantlets were obtained either by transferring the callus to light on an auxin-free medium containing kinetin or PBA, or from young anthers. Adventitious organ formation in explants and callus was closely related to the auxin/cytokinin ratio in the medium, rooting being promoted by auxins and bud formation by cytokinins. Root and bud formation were greater in light than in darkness. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals Vegetative propagation of Freesia through the isolation of shoots in vitro.

1976 ◽  
Vol 24 (4) ◽  
pp. 274-277
Author(s):  
R.L.M. Pierik ◽  
H.H.M. Steegmans
Keyword(s):  
Vegetative Propagation ◽  
Flower Buds ◽  
Skoog Medium ◽  
Golden Yellow ◽  
Murashige And Skoog Medium ◽  
Number Of Shoots

Shoots from the cvs Ballerina, Aurora, Rijnveld's Golden Yellow and Rose Marie, originating from excised flower-buds, were grown in vitro and were induced to form shoots on a modified Murashige and Skoog medium containing PBA and IAA. The greatest number of new shoots/excised shoot was obtained when the medium contained PBA at 5 mg/l and IAA at 0.1 mg/l. The number of shoots formed depended on the cv. tested. Subcultured shoots, grown on a medium with IAA but without PBA, could be rooted easily and viable plants were obtained. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals Factors influencing flower bud formation on the pear tree cultivar 'Doyenne du Comice'. I. Shoot growth and flower bud differentiation on trees sprayed with chlormequat and unsprayed ones

2013 ◽  
Vol 36 (1-2) ◽  
pp. 83-94 ◽  
Author(s):  
Franciszka Jaumień
Keyword(s):  
Shoot Growth ◽  
Apple Tree ◽  
Flower Buds ◽  
Flower Bud ◽  
Bud Formation ◽  
Pear Tree ◽  
Factors Influencing ◽  
Long Shoots ◽  
Flower Bud Differentiation ◽  
Flower Bud Formation

The growth of trees sprayed in spring with chlormequat is weaker, and their elongation growth ends 2 - 3 weeks earlier than that of unsprayed trees. Trees with growth inhibited by chlormequat set flower buds on the spurs and in the subapical part of long shoots. The course of flower bud differentiation starts in the second half of July and is similar to that in the apple tree.

Micropropagation of Scaevola —Australian native of ornamental horticulture

1998 ◽  
Vol 38 (4) ◽  
pp. 399 ◽  
Author(s):  
P. L. Bhalla ◽  
K. Sweeney
Keyword(s):  
Tissue Culture ◽  
Butyric Acid ◽  
High Survival ◽  
Root Initiation ◽  
Free Medium ◽  
Skoog Medium ◽  
Survival Percentage ◽  
Murashige And Skoog Medium ◽  
Ornamental Horticulture

Summary. A number of commercially available cultivars of Scaevola aemula, S. albida, S. phlebopetala, S. striata and material collected from the wild of S. glandulifera, S. hookeri and S. ramonissima were successfully propagated by tissue culture. Shoot segments 3–4 cm in length were multiplied in Murashige and Skoog medium without hormones. Addition of 25–150 µmol kinetin/L in the micropropagation medium of S. aemula and S. phlebopetala resulted in the formation of deformed shoots. Tissue cultured shoots rooted in hormone-free medium in 4–6 weeks. Indole-3-butyric acid (10–20 µmol/L) had an effect on rate of root initiation of S. phlebopetala but not on percentage of rooting. A high survival percentage (>95%) was obtained when plants were transferred to soil under glasshouse conditions indicating that micropropagation of Scaevola is feasible.

Effects of nitrogen addition on the population dynamics and flowering of Pulsatilla vernalis

1993 ◽  
Vol 71 (5) ◽  
pp. 732-736 ◽  
Author(s):  
Olle Kellner
Keyword(s):  
Population Dynamics ◽  
Key Words ◽  
Nitrogen Addition ◽  
Permanent Plots ◽  
Flower Buds ◽  
Flower Bud ◽  
Bud Formation ◽  
Single Fertilization ◽  
Flower Bud Formation ◽  
And Control

A population of Pulsatilla vernalis (L.) Mill, was studied in permanent plots for 8 years. Half the plots were fertilized with ammonium nitrate (15 g N/m2) at the start of the study. Mortality and natality in the ramet population were very low in both fertilized and control treatments. In contrast, there was a considerable between-year turnover of shoots within ramets in both treatments. The number of shoots increased threefold after fertilization and then gradually decreased to the original level. Flower-bud formation in unfertilized plots was lower in warm and dry years than in cooler, wetter ones. In fertilized plots, flower-bud formation was higher after fertilization irrespective of the climate at the time. The proportion of flower buds grazed was higher in fertilized plots. Even though the species is confined to nitrogen-poor habitats, a single fertilization did not adversely affect its performance. Key words: Pulsatilla vernalis, population dynamics, nitrogen, weather, forestry.

THE EFFECTS OF PHOTOPERIOD, TEMPERATURE, AND LIGHT INTENSITY ON THE GROWTH OF THE LOWBUSH BLUEBERRY (VACCINIUM ANGUSTIFOLIUM AIT.)

1961 ◽  
Vol 39 (7) ◽  
pp. 1733-1739 ◽  
Author(s):  
I. V. Hall ◽  
R. A. Ludwig
Keyword(s):  
Environmental Factors ◽  
Vegetative Growth ◽  
Photoperiodic Response ◽  
Day Length ◽  
Flower Buds ◽  
Flower Bud ◽  
Bud Formation ◽  
Lowbush Blueberry ◽  
Flower Bud Formation ◽  
Field And Laboratory Conditions

A study of the effect of environmental factors on the growth and development of the lowbush blueberry was carried out using clonally propagated plants. In a preliminary study a definite photoperiodic response was found. Under 8-hour days flower buds were formed and no vegetative growth occurred. Under 16-hour days vegetative growth resulted and no flower bud formation occurred. In a replicated greenhouse experiment, seven clones produced flower buds with 8-, 10-, and 12-hour days, but produced none with 14- or 16-hour days. One clone produced flower buds with 8-, 10,- 12-, and 14-, but none with 16-hour days. Two clones were able to produce flower buds under all five photoperiods. Under 8- and 10- hour photoperiods no vegetative growth occurred. Under 12, 14, and 16 hours progressively more vegetative growth occurred. In an experiment on the interaction of temperature and photoperiod, vegetative growth was significantly greater at 70° F than at 50° F with the differences being accentuated by day length. Flower bud formation occurred with 11- and 13-hour photoperiods regardless of temperature, but was more pronounced at the higher temperatures. At 70° F, 15-hour photoperiod, no flower buds were formed while at 50° F, 15 hours, three clones produced no flower buds and six clones produced an abnormal type of inflorescence. Similar abnormal inflorescences were produced by giving plants 2, 3, or 4 weeks of 8-hour days. Six weeks of 8-hour days was sufficient to initiate normal inflorescences. Shade, provided by two layers of cheesecloth, significantly reduced the number of flower buds compared with full sunlight. The growth of the lowbush blueberry under field and laboratory conditions is discussed in relation to environmental factors.

USE OF GIBBERELLIC ACID TO HASTEN FLOWERING IN RUTABAGA

1982 ◽  
Vol 62 (3) ◽  
pp. 823-826 ◽  
Author(s):  
A. ALI ◽  
V. SOUZA MACHADO
Keyword(s):  
Seed Production ◽  
Rapid Development ◽  
Cold Treatment ◽  
Flower Buds ◽  
Flower Bud ◽  
Breeding Programs ◽  
Bud Formation ◽  
Growth Room ◽  
Flower Bud Formation

In field conditions, rutabaga (Brassica napus ssp. Rapifera (Metzg.) Sinsk.) plants are biennials and require exposure to low temperature for completion of their life cycle to seed production state. When young rutabaga plants were thermoinduced (3–5 °C) for 8 wk and subsequently transferred to growth room conditions, formation of flower buds resulted in 6 wk. Flowering response was greatly enhanced if the plants were sprayed with GA3 (100 mg/L) prior to thermoinduction. Compared with untreated plants, the GA3-sprayed plants responded with flower bud formation after as little as 3 wk of cold treatment. A longer cold exposure (6–8 wk) of the GA3-sprayed plants resulted in rapid development and maturity of the inflorescence. This note emphasizes the pharmacological role of gibberellins as regulators of flowering and their usefulness to enhance seed production and plant breeding programs.

scholarly journals Early Defoliation Reduces Flower Bud Counts on Rabbiteye Blueberry

HortScience ◽  
1992 ◽  
Vol 27 (7) ◽  
pp. 783-785 ◽  
Author(s):  
Paul M. Lyrene
Keyword(s):  
Flower Buds ◽  
Flower Bud ◽  
Bud Formation ◽  
Flower Bud Formation ◽  
Commercial Field

Vigorous, upright shoots on mature V. ashei Reade cv. Aliceblue plants growing in a commercial field planting were used to study the effects of premature defoliation on flower bud formation. Three treatments (total shoot defoliation, alternate-node defoliation, and no defoliation) were applied on each of three dates (20 Aug., 17 Sept., and 15 Oct. 1987). For the August defoliation, the number of flower buds present per shoot on 6 Jan. of the following year averaged 1.3 for shoots that were totally defoliated, 3.7 for shoots on which alternate nodes had been defoliated, and 4.2 for control (nondefoliated) shoots. Shoots treated on 17 Sept. averaged 2.6 buds per shoot for total defoliation, 4.1 for alternate-node defoliation, and 4.8 for controls. Defoliation on 15 Oct. did not reduce flower bud formation. Reduction in flower bud formation due to defoliation was localized at the defoliated nodes. For shoots on which alternate nodes were defoliated on 20 Aug., 59.8% of the apical five nodes that were not defoliated produced flower buds compared with 1.4% of the defoliated nodes.

scholarly journals Flower Bud Formation of Sacred Lotus (Nelumbo nucifera Gaertn.): A Case Study of ‘Gyozankouren’ Grown in a Container

HortScience ◽  
2014 ◽  
Vol 49 (4) ◽  
pp. 516-518 ◽  
Author(s):  
Fumiko Ishizuna ◽  
Nobuhiro Tsutsumi
Keyword(s):  
Floral Development ◽  
Flower Buds ◽  
Flower Bud ◽  
Edible Plant ◽  
Abaxial Side ◽  
Bud Formation ◽  
Sacred Lotus ◽  
Foliage Leaf ◽  
Flower Bud Formation

The genus Nelumbo consists of two species, N. nucifera and N. lutea. N. nucifera is an ornamental and edible plant that is widely cultivated. Earlier studies of sacred lotus (N. nucifera) flowers focused mainly on morphology, phyllotaxis, leaf arrangements, and flower development. During the growing season, sacred lotus produces one foliage leaf at each node. Flower buds emerge from the abaxial side of the basal part of the foliage leaf. However, the number of blooming flowers is much less than the number of foliage leaves. Little is known concerning flower bud formation during lotus plant development. This is the first experimental study to reveal that every node has one flower bud even in the dormant shoot apex and that most of the formed flower buds aborted in the course of floral development. Our results suggest that flower bud formation of sacred lotus is independent of daylength. On the other hand, whether a formed bud reaches blooming may depend on environmental factors.

scholarly journals Future outlook of plant growth regulators application influencing callus induction from different type explant Hyoscyamus muticus L. in vitro

2018 ◽  
Vol 7 (3) ◽  
pp. 10-13
Author(s):  
Walla Abdelmaksood Abdelazeez ◽  
Landysh Zavdetovna Khusnetdinova ◽  
Olga Arnoldovna Timofeeva
Keyword(s):  
Callus Induction ◽  
Nutrient Medium ◽  
Callus Formation ◽  
Stem Explants ◽  
Hyoscyamus Muticus ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Naphthylacetic Acid ◽  
Egyptian Henbane

The article shows the results concerning the problem of the influence of the hormonal composition of the medium on callus induction in isolated from different explants of Egyptian henbane areas (on the example of Hyoscyamus muticus L.). The authors study 11 variants of Murashige and Skoog medium supplemented with different concentrations and combination of auxins and cytokinins. It was important to find nutrient medium modification of Murashige and Skoog for callus induction. The article describes the fact that callus formation from different explant types of Hyoscyamus muticus L. in vitro was observed on Murashige and Skoog medium fortified with benzylaminopurine and naphthylacetic acid. It shows that the maximum callus induction was observed from root explants on Murashige and Skoog's medium supplemented with 0.5 mg/l of benzylaminopurine and 1.0 mg/l of naphthylacetic acid. And minimal callus formation was observed in the area with benzylaminopurine. Callus induction of leaf and stem explants both on the hormone-free nutrient medium and with the benzylaminopurine only was not observed. Thus, the results show that the frequency of callus formation with culturing root segment is higher compared to leaf and stem segment explants (on the example of Egyptian henbane in culture in vitro ). This work aims to inducing callus formation from various explants of Egyptian henbane, which can be used for plant regeneration or as a source for in vitro production of secondary metabolites.

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