Micropropagation of Scaevola —Australian native of ornamental horticulture

1998 ◽  
Vol 38 (4) ◽  
pp. 399 ◽  
Author(s):  
P. L. Bhalla ◽  
K. Sweeney
Keyword(s):  
Tissue Culture ◽  
Butyric Acid ◽  
High Survival ◽  
Root Initiation ◽  
Free Medium ◽  
Skoog Medium ◽  
Survival Percentage ◽  
Murashige And Skoog Medium ◽  
Ornamental Horticulture

Summary. A number of commercially available cultivars of Scaevola aemula, S. albida, S. phlebopetala, S. striata and material collected from the wild of S. glandulifera, S. hookeri and S. ramonissima were successfully propagated by tissue culture. Shoot segments 3–4 cm in length were multiplied in Murashige and Skoog medium without hormones. Addition of 25–150 µmol kinetin/L in the micropropagation medium of S. aemula and S. phlebopetala resulted in the formation of deformed shoots. Tissue cultured shoots rooted in hormone-free medium in 4–6 weeks. Indole-3-butyric acid (10–20 µmol/L) had an effect on rate of root initiation of S. phlebopetala but not on percentage of rooting. A high survival percentage (>95%) was obtained when plants were transferred to soil under glasshouse conditions indicating that micropropagation of Scaevola is feasible.

scholarly journals Studies on in Vitro Culture of the Australian Fan Flower, Scaevola

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 548B-548
Author(s):  
Prem L. Bhalla ◽  
Katherine Tozer
Keyword(s):  
In Vitro Culture ◽  
Stem Explants ◽  
High Survival ◽  
Shoot Induction ◽  
Free Medium ◽  
Direct Shoot Regeneration ◽  
Important Species ◽  
Survival Percentage ◽  
Regeneration Response

Plants of genus Scaevola (family, Goodeniaceae), commonly known as “fan flowers,” are mostly endemic to Australia. Commercially popular species are Scaevola aemula, S. albida, S. striata, and S. phlebopetala. These plants are used as ground covers in Australia and as hanging baskets, window boxes, and garden bed plants in Europe and America. Two aspects of in vitro culture of Scaevola are reported here; micropropagation and direct shoot regeneration. A number of commercially available cultivars of S. aemula, S. phlebopetala, S. striata and wild-collected S. phlebopetala, S. glandulifera, S. hookeri, and S. ramonissima were used for micropropagation experiments. Micropropagation medium contained salts, vitamins, L-cysteine, sucrose, and agar. Tissue-cultured shoots were rooted in hormone-free medium. A high survival percentage (>95%) was obtained when plants were transferred to soil under glasshouse conditions. Results on in vitro shoot induction and regeneration response of leaf, stem, root, node, and flower explants of two horticulturally important species of the Australian fan flower, Scaevola aemula and Scaevola striata arealso presented. Of all the explants tested, node explants of these species were the first to respond in tissue culture. Maximum number of shoot induction and regeneration was achieved from node explants of Scaevola aemula and node and stem explants of Scaevola striata. More than 95% of the regenerated shoots were rooted on the medium supplemented with 4 mg/L of IBA. The significance of above findings in assisting breeding program for new horticultural desirable cultivars of Australian fan flowers will be discussed.

scholarly journals In Vitro Propagation of Pandoreas

HortScience ◽  
2001 ◽  
Vol 36 (2) ◽  
pp. 348-350 ◽  
Author(s):  
S. Latha Kancherla ◽  
Prem L. Bhalla
Keyword(s):  
Tissue Culture ◽  
Butyric Acid ◽  
In Vitro Propagation ◽  
Shoot Formation ◽  
Nodal Segments ◽  
Shoot Induction ◽  
Indole Butyric Acid ◽  
Commercial Cultivars ◽  
Murashige And Skoog Medium

Pandoreas, Australian natives of horticultural significance, were successfully propagated using tissue culture. A protocol for rapid in vitro multiplication of commercial cultivars was developed using nodal segments cultured on Murashige and Skoog medium containing either BA or kinetin. Maximum shoot induction and number of shoots per explant for P. pandorana (Andrews) Steenis and P. jasminoides (Lindley) Schumann were on 8.8 μm BA and 4.6 μm kinetin. Higher levels of cytokinin in the medium inhibited shoot formation. Tissue-cultured shoots were rooted with IBA. This study demonstrates that Pandoreas can be successfully micropropagated. Chemical names used: 6-benzylaminopurine (BA); 3-indole butyric acid (IBA).

scholarly journals Micropropagation of Virginia Sweetspire (Itea virginica ‘Henry's Garnet’)

2003 ◽  
Vol 21 (4) ◽  
pp. 206-208
Author(s):  
Jon T. Lindstrom ◽  
Matthew C. Pelto
Keyword(s):  
Tissue Culture ◽  
Shoot Induction ◽  
Ex Vitro ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
One Year

Abstract The woody shrub, Itea virginica L., Virginia sweetspire, has recently increased in popularity due to its multiple seasons of interest in the landscape. In this study, we investigated micropropagation as a means to produce this plant. Combinations of BA (1, 4, and 10 μM) and NAA (0.01, 0.1, and 1.0 μM) were evaluated for in vitro shoot induction in Itea virginica L. ‘Henry's Garnet’ on a Murashige and Skoog medium. The best combination of BA and NAA (4 μM and 0.1 μM) yielded an average of 7.9 microshoots per explant for ‘Henry's Garnet’. When dipped in a common auxin-containing, commercial rooting formulation, microshoots rooted ex vitro within four weeks. Tissue-culture produced plantlets of I. virginica ‘Henry's Garnet’ flowered one year after removal from culture.

scholarly journals Vegetative propagation of freesia through callus cultures.

1974 ◽  
Vol 22 (3) ◽  
pp. 153-159
Author(s):  
Y.P.S. Bajaj ◽  
R.L.M. Pierik
Keyword(s):  
Callus Induction ◽  
Vegetative Propagation ◽  
Callus Cultures ◽  
Free Medium ◽  
Flower Buds ◽  
Flower Bud ◽  
Bud Formation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Stem Leaf

Isolated segments of corm, stem, leaf, flower bud and anther from flowering freesia plants, cv. Ballerina, were induced to form callus on a modified Murashige and Skoog medium supplemented with NAA and 6-(benzylamino)-9-(2-tetrahydropyranyl)-9H-purine (PBA). Callus induction was best with young flower buds kept at 25 deg C in darkness. Callus could be sub-cultured in darkness on a medium containing auxin and cytokinin. Complete plantlets were obtained either by transferring the callus to light on an auxin-free medium containing kinetin or PBA, or from young anthers. Adventitious organ formation in explants and callus was closely related to the auxin/cytokinin ratio in the medium, rooting being promoted by auxins and bud formation by cytokinins. Root and bud formation were greater in light than in darkness. (Abstract retrieved from CAB Abstracts by CABI’s permission)

Plantlet production from anthers of Eastern cottonwood (Populusdeltoïdes)

1988 ◽  
Vol 18 (7) ◽  
pp. 937-941 ◽  
Author(s):  
M. Rafique Uddin ◽  
Martin M. Meyer Jr. ◽  
J. J. Jokela
Keyword(s):  
Butyric Acid ◽  
Woody Plant ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Eastern Cottonwood ◽  
Skoog Medium ◽  
Plantlet Production ◽  
Murashige And Skoog Medium ◽  
Woody Plant Medium ◽  
2,4 Dichlorophenoxyacetic Acid

Plantlets were obtained by organogenesis from cultured anthers of Populusdeltoides (Bartr.). Anthers formed callus in the dark on modified Murashige and Skoog medium supplemented with 9.0 μM 2,4-dichlorophenoxyacetic acid and 4.7 μM kinetin. Anther calli were differentiated into shoots by sequential transfer in the light onto Murashige and Skoog medium containing 4.4 μM benzylamino purine and 1.1 μM naphthaleneacetic acid for 4 weeks, followed by several transfers to woody plant medium with 2.2 μM benzylamino purine and 1.1 μM naphthaleneacetic acid. The shoots that formed were rooted by excising and transferring to woody plant medium supplemented with 1.0 μM indole-3-butyric acid. A few of these plants were found to be haploid. Two plants developed male terminal inflorescences, but died shortly thereafter.

scholarly journals Effect of the Different Concentrations of Protective-stimulating Complex (HFC) on the Growth and Development of Grape Seedlings in the Tissue Culture at the Different Nutrient Levels

2021 ◽  
Author(s):  
Esraa M.M. Farahat ◽  
S.L. Belopukhov
Keyword(s):  
Tissue Culture ◽  
Growth And Development ◽  
In Vitro Propagation ◽  
Vital Role ◽  
Controlled Environment ◽  
Skoog Medium ◽  
Nutrient Levels ◽  
Murashige And Skoog Medium ◽  
Plant Seedlings

Background: Humic substances plays a vital role in the plant tissue culture as a growth hormone for in vitro propagation of many plant seedlings. The aim of this study was to investigate the effect of added humic-fulvate complex (HFC) at the various concentrations on the growth and development of grape seedlings in in vitro at the different nutrient levels. Methods: The cutting of khasansky grape were cultivated on ¼ Murashige and Skoog medium or ½ Murashige and Skoog medium either alone or supplemented with the humic-fulvate complex at the different concentrations at (0.1, 1 and 10 ml/l). Then, they were cultured for 4 weeks under a controlled environment. Result: The data observed that the low concentration of Murashige and Skoog medium (¼ MS) for in vitro rooting of grape cv. ‘Khasansky’ either alone or combined with HFC at the various concentrations significantly increased the rooting percentages and the total length of roots and stimulating the rate of vegetative growth compared with cultivated in ½ MS medium either alone or with supplemented with HFC. ¼ MS+ 10 ml/l HFC was the best treatment for improving the growth of khasansky grape seedlings.

scholarly journals Tissue Culture Propagation of Yam in Puerto Rico

1969 ◽  
Vol 67 (4) ◽  
pp. 419-428
Author(s):  
Amelia Cortés-Monllor ◽  
Lii-Jang Liu
Keyword(s):  
Tissue Culture ◽  
Puerto Rico ◽  
Indoleacetic Acid ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Dioscorea Rotundata ◽  
Skoog Medium ◽  
Rooting Medium ◽  
Murashige And Skoog Medium ◽  
Tissue Culture Propagation

Rapid propagation of yam (Dioscorea rotundata cv. Habanero) was obtained from nodal segments cultured in modified Murashige and Skoog medium containing indoleacetic acid (IAA) and kinetin as establishment medium, and naphthaleneacetic acid (NAA) as rooting medium. The proliferation cycle, which takes approximately 2-3 months, increased four times the production of yam plantlets. These plantlets were successfully transferred to potting mixture and soil. This procedure is extremely useful for regenerating virusfree plantlets suitable for producing healthy tubers for planting.

scholarly journals Plant Regeneration from Anther-derived Embryos of Apple and Pear

HortScience ◽  
2002 ◽  
Vol 37 (6) ◽  
pp. 962-965 ◽  
Author(s):  
Masanori Kadota ◽  
Dong-Sheng Han ◽  
Yoshiji Niimi
Keyword(s):  
Plant Regeneration ◽  
Butyric Acid ◽  
Callus Formation ◽  
Adventitious Shoots ◽  
Pyrus Pyrifolia ◽  
Chromosome Counts ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Half Strength ◽  
Apple Cultivars

Anthers of six apple [Malus ×domestica (L.) Borkh.], three Japanese pear (Pyrus pyrifolia N.) and two European pear (Pyrus communis L.) scion cultivars were cultured. Callus formation occurred from anthers of all cultivars and androgenic embryogenesis was observed from all except P. pyrifolia `Kosui' and P. communis `La France'. Regeneration of adventitious shoots from anther-derived embryos was shown from all apple cultivars and P. pyrifolia `Shinko'. Many of these shoots did not grow or died on half-strength Murashige and Skoog medium (1962) with 4.4 μm BA and 0.5 μm IBA, whereas several shoots of apple `Starking Delicious' grew to plantlets. Chromosome counts of shoot apical cells of four clones derived from embryos of `Starking Delicious' showed that three clones were diploids and one clone comprised diploid and haploid shoots, suggesting that at least one clone originated from a microspore. Chemical names used: 3-indolyl-butyric acid (IBA); N6-benzyladenine (BA).

scholarly journals The role of JrPPOs in the browning of walnut explants

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shugang Zhao ◽  
Hongxia Wang ◽  
Kai Liu ◽  
Linqing Li ◽  
Jinbing Yang ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Production Efficiency ◽  
Phenolic Content ◽  
Leaf Tissue ◽  
Limiting Factor ◽  
Mature Leaves ◽  
Survival Percentage ◽  
Highly Active ◽  
Young Leaves

Abstract Background Tissue culture is an effective method for the rapid breeding of seedlings and improving production efficiency, but explant browning is a key limiting factor of walnut tissue culture. Specifically, the polymerization of PPO-derived quinones that cause explant browning of walnut is not well understood. This study investigated explants of ‘Zanmei’ walnut shoot apices cultured in agar (A) or vermiculite (V) media, and the survival percentage, changes in phenolic content, POD and PPO activity, and JrPPO expression in explants were studied to determine the role of PPO in the browning of walnut explants. Results The results showed that the V media greatly reduced the death rate of explants, and 89.9 and 38.7% of the explants cultured in V media and A media survived, respectively. Compared with that of explants at 0 h, the PPO of explants cultured in A was highly active throughout the culture, but activity in those cultured in V remained low. The phenolic level of explants cultured in A increased significantly at 72 h but subsequently declined, and the content in the explants cultured in V increased to a high level only at 144 h. The POD in explants cultured in V showed high activity that did not cause browning. Gene expression assays showed that the expression of JrPPO1 was downregulated in explants cultured in both A and V. However, the expression of JrPPO2 was upregulated in explants cultured in A throughout the culture and upregulated in V at 144 h. JrPPO expression analyses in different tissues showed that JrPPO1 was highly expressed in stems, young leaves, mature leaves, catkins, pistils, and hulls, and JrPPO2 was highly expressed in mature leaves and pistils. Moreover, browning assays showed that both explants in A and leaf tissue exhibited high JrPPO2 activity. Conclusion The rapid increase in phenolic content caused the browning and death of explants. V media delayed the rapid accumulation of phenolic compounds in walnut explants in the short term, which significantly decreased explants mortality. The results suggest that JrPPO2 plays a key role in the oxidation of phenols in explants after branch injury.

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