scholarly journals Comparison of direct total iron binding capacity by light MgCO3 and heavy MgCO3

2021 ◽  
Vol 9 (6) ◽  
pp. 1703
Author(s):  
Chapal Debnath ◽  
Niral G. Savaliya
Keyword(s):  
Binding Capacity ◽  
Magnesium Carbonate ◽  
Sample Volume ◽  
Complete Analysis ◽  
Iron Binding ◽  
Positive Bias ◽  
Patient Identification ◽  
Adsorption Measurement ◽  
Using Data ◽  
Iron Binding Capacity

Background: The aim of the study is focused to measure TIBC by calculated method (TIBC using the magnesium carbonate- MgCO3 adsorption), measurement of TIBC by calculatory method from automated UIBC and automated Iron measurement. Latter these two values are to be added and would give an additive result of TIBC. The experiment would be performed by using the light MgCO3 powder and the heavy MgCO3 powder and check for any differences in their respective values. Regression analysis, Bland Altman analysis or histogram analysis of TIBC results obtained by calculated methods in patient samples to find correlation between the two methods is being performed.Methods: Samples received for analysis of tests, regardless of patient identification with sample volume serum were used for the study. After complete analysis and reporting of the sample, the leftover serum was used. Serum was kept at room temperature. Then the calculated TIBC from UIBC and the IRON were compared after doing batch calibration for UIBC and lot calibration of Iron.Results: It was found that, this study had positive bias by the usage of the heavy MgCO3 powder. Positive bias in unsaturated iron binding capacity observed by both methods is studied and further investigated using data obtained during the experiment.Conclusions: There is a higher yield of serum obtained with the heavy MgCO3 powder, TIBC calculated from iron and unsaturated iron binding capacity as compared to TIBC measured directly using the light MgCO3 powder.

Ferrozine iron and total iron-binding capacity method adapted to the ABA-100 Bichromatic Analyzer.

1981 ◽  
Vol 27 (8) ◽  
pp. 1441-1444 ◽  
Author(s):  
L Mori ◽  
A Bekkering ◽  
J Traini ◽  
L Vanderlinden
Keyword(s):  
Binding Capacity ◽  
Magnesium Carbonate ◽  
Total Iron ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Standard Curve ◽  
Excess Iron ◽  
Color Reagent ◽  
Iron Binding Capacity ◽  
Capacity Method

Abstract A sensitive method (Clin. Chem. 26: 327--331, 1980) for serum iron, in which the color reagent Ferrozine is used, is modified and adapted to the Abbott ABA-100 discrete analyzer. The standard curve is linear to at least 10 mg/L and the method showed day-to-day precision (CV) of 2.4% for a 1.03 mg/L sample (n = 63) and 1.9% for a 2.13 mg/L sample (n = 63). Lower values were obtained than with the modified continuous-flow technique of Giovanniello et al., but the correlation was good (r = 0.98). Bilirubin and copper do not interfere; hemoglobin and gross lipemia interfere only slightly. The total iron-binding capacity, based on Ramsay's method, was evaluated with regard to the effect of adding various amounts of magnesium carbonate. Results led us to use a ratio of approximately 180 mg of magnesium carbonate to each 5 micrograms of excess iron added. Day-to-day, the method for total iron-binding capacity gave a CV of 3.1% for a 2.55 mg/L sample, 2.8% for a 3.63 mg/L sample.

An AutoAnalyzer Procedure for Serum Iron and Total Iron-Binding Capacity, with Use of Ferrozine

1971 ◽  
Vol 17 (9) ◽  
pp. 950-953 ◽  
Author(s):  
Hugh Y Yee ◽  
Anastasia Zin
Keyword(s):  
Serum Iron ◽  
Binding Capacity ◽  
Sample Volume ◽  
Total Iron ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Iron Binding Capacity

Abstract An AutoAnalyzer method has been developed in which the recently introduced reagent for iron, 3-(2-pyridyl)-5,6-bis(4-phenylsulfonic acid)-1,2,4-triazine ("ferrozine"), is used. With the Technicon colorimeter or Gilford 300-N spectrophotometer in use, sampling rates may be 30/h or 50/h, respectively. Sample volume is 0.8 ml, and measurements are made at 560 nm. A procedure for measuring total iron-binding capacity is also described.

Macro and Micro Methods for the Determination of Serum Iron and Iron-Binding Capacity

1963 ◽  
Vol 9 (2) ◽  
pp. 188-199 ◽  
Author(s):  
Wendell T Caraway
Keyword(s):  
Ammonium Acetate ◽  
Binding Capacity ◽  
Magnesium Carbonate ◽  
Iron Binding ◽  
Colored Complex ◽  
Iron Proteins ◽  
Excess Iron ◽  
Iron Binding Capacity ◽  
Clear Supernatant

Abstract Blood serum is incubated with ascorbic acid and hydrochloric acid to liberate protein-bound iron. Proteins are precipitated with trichloroacetic acid in the presence of chloroform. To an aliquot of clear supernatant is added a solution of tripyridyltriazine and sufficient ammonium acetate to adjust the ρH between 4 and 5. Absorbance of the blue-colored complex is measured at 590 m µ . Iron-binding capacity is measured by saturation of serum with ferric iron followed by removal of excess iron with magnesium carbonate. Measurement of total bound iron is completed as above. Macro and micro technics for either determination require 2 ml. and 0.1 ml. of serum, respectively. Reliability of the methods has been established by recovery experiments, replicate analyses, and variation of reagent concentrations and incubation times.

Use of an alumina column in estimating total iron-binding capacity.

1980 ◽  
Vol 26 (1) ◽  
pp. 156-158 ◽  
Author(s):  
R T Starr
Keyword(s):  
Regression Analysis ◽  
Binding Capacity ◽  
Magnesium Carbonate ◽  
Total Iron ◽  
Negative Bias ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Alumina Column ◽  
Excess Iron ◽  
Iron Binding Capacity

Abstract I describe a simple, rapid technique for saturating the transferrin in serum and then removing unbound (excess) iron. This technique involves use of an easily prepared column of basic chromatographic alumina and a saturating solution of ferric chloride in citric acid. This method, when compared with the magnesium carbonate method of Ramsay (Clin. Chim. Acta 2: 221, 1957) by regression analysis and tests of precision, showed a negative bias for results by the alumina technique with respect to the magnesium carbonate method.

Use of an alumina column in estimating total iron-binding capacity.

1980 ◽  
Vol 26 (1) ◽  
pp. 156-158 ◽  
Author(s):  
R T Starr
Keyword(s):  
Regression Analysis ◽  
Binding Capacity ◽  
Magnesium Carbonate ◽  
Total Iron ◽  
Negative Bias ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Alumina Column ◽  
Excess Iron ◽  
Iron Binding Capacity

Abstract I describe a simple, rapid technique for saturating the transferrin in serum and then removing unbound (excess) iron. This technique involves use of an easily prepared column of basic chromatographic alumina and a saturating solution of ferric chloride in citric acid. This method, when compared with the magnesium carbonate method of Ramsay (Clin. Chim. Acta 2: 221, 1957) by regression analysis and tests of precision, showed a negative bias for results by the alumina technique with respect to the magnesium carbonate method.

Sign in / Sign up

Export Citation Format

Share Document