Immunohistochemical expression of P16 and Ki-67 in uterine cervical neoplasms

2021 ◽  
Vol 6 (3) ◽  
pp. 194-200
Author(s):  
Kafil Akhtar ◽  
Shafaque Zabin ◽  
Zehra Mohsin ◽  
Shahid A Siddiqui
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Uterine Cervix ◽  
Invasive Carcinoma ◽  
Large Cell ◽  
Carcinoma Cervix ◽  
Ki 67 ◽  
Early Detection Of Disease ◽  
Keratinizing Squamous Cell Carcinoma

To study the expression of Ki-67 and p16 in neoplastic lesions of uterine cervix and to evaluate the prognostic significance of tumour differentiation, histological type, stage and grade, depth of tumour invasion and lymphovascular invasion in women with neoplasia of uterine cervix. This study was performed on 50 biopsies and surgically resected specimens of uterine cervix, which were fixed in 10% formalin, processed, paraffin embedded and cut into 3-5 microns thickness, stained with hematoxylin and eosin stains and immunohistochemical staining by p16 and Ki-67 antibodies was performed and studied. Majority of cases of invasive carcinoma cervix were large cell non-keratinizing squamous cell carcinoma, 58 cases (48.2%). Majority of cases of invasive carcinoma cervix were seen in stage 2B, 40 (40.8%) cases followed by stage 3B, 24 (24.4%) cases. Out of 18 cases of large cell non-keratinizing squamous cell carcinoma, 12 (66.7%) showed 3+ positivity for Ki-67. 7 (53.8%) cases of stage 2A showed 3+ positivity, 3 (23.1%) cases each showed 2+ positivity for p16. 7 (36.8%) cases of moderately differentiated SCC showed 3+ positivity, 4 (21.1%) showed 2+ positivity and 5 (26.3%) showed 1+ positivity of Ki-67. Out of 10 stable patients, 4 (40.0%) showed negative p16 immunoexpression and all the 3 cases (100%) with recurrence showed 2+ positivity and 2 (100%) patients with metastasis showed 3+ positivity. Out of 10 stable patients, 6 (60%) showed negative Ki-67 immunoexpression and all the 3 (100%) cases with recurrence showed 3+ positivity and one each (50.0%) patient with metastasis showed 2+ positivity and 3+ positivity. p16 and Ki-67 immunomarkers are useful as a diagnostic and prognostic tool in cases with recurrence and metastasis, helping in early detection of disease progression.

scholarly journals Primary histiocytic sarcoma of the uterine cervix: an extremely rare entity

2017 ◽  
Vol 55 (1) ◽  
pp. 53-56 ◽  
Author(s):  
Fariba Binesh ◽  
Mojgan Karimi-Zarchi ◽  
Mohammad Reza Vahidfar ◽  
Zahra Kargar Hadgiabadi
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Uterine Cervix ◽  
Large Cell ◽  
Histiocytic Sarcoma ◽  
Pathological Examination ◽  
Keratinizing Squamous Cell Carcinoma ◽  
Post Menopausal ◽  
Cervical Tumors

Abstract Objective. Histiocytic sarcoma (HS) is an extraordinary rare tumor and it has an offensive clinical course. HS of the uterine cervix is a far uncommon tumor with just a few cases described so far. Here we presented a case of primary HS of the uterine cervix in a 62-year-old female initially misdiagnosed as large cell non-keratinizing squamous cell carcinoma. To the best of our knowledge, this is the first reported case of HS of the uterine cervix from Iran. Case report. The patient presented with post-menopausal vaginal bleeding. Further evaluations revealed the presence of a relatively large cervical mass that was sampled. Under the preliminary diagnosis of large cell non-keratinizing squamous cell carcinoma, the patient underwent radical surgery. Postoperative pathological examination confirmed the diagnosis of HS of the uterine cervix. The patient received chemotherapy. Conclusion. Clinical presentations of HS of the uterine cervix are very similar to more common cervical tumors; as a result its diagnosis relies on immunohistochemical methods. A correct diagnose could lead to the proper and timely treatment.

scholarly journals Advanced Composite of Large Cell Neuroendocrine Carcinoma and Squamous Cell Carcinoma: A Case Report of Uterine Cervical Cancer in a Virgin Woman

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Ryusuke Murakami ◽  
Iemasa Kou ◽  
Kenjiro Date ◽  
Hirofumi Nakayama
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Tumor Cells ◽  
Neuroendocrine Carcinoma ◽  
Squamous Cell ◽  
Uterine Cervix ◽  
Neuron Specific Enolase ◽  
Large Cell ◽  
Large Cell Neuroendocrine Carcinoma ◽  
Hpv Test

Large cell neuroendocrine carcinoma (LCNEC) of the uterine cervix is very rare and aggressive. The prognosis is very poor despite multimodal treatment. We report a virgin woman with FIGO stage 4b LCNEC of uterine cervix coexisting with squamous cell carcinoma. An early thirties virgin woman presented with 2-month history of abdominal pain. A chest X-ray showed multiple lung metastatic tumors. A vaginal smear showed malignant cells, and a biopsy specimen had features of LCNEC. The tumor showed trabecular patterns. Tumor cells possessed a moderate amount of cytoplasm, prominent nucleoli, and large nuclei. The tumor cells are stained positive for synaptophysin, chromogranin A, and neuron specific enolase (NSE). The invasive tumor cells in connection with cervical squamous epithelium were focally positive for 34bE12. We made a diagnosis of composite LCNEC and nonkeratinizing squamous cell carcinoma. High-risk HPV test was negative with hybridized captured method 2.

scholarly journals HISTOPATHOLOGICAL EVALUATION OF CERVICAL LESIONS IN TERTIARY BASED HOSPITAL - KUTCH REGION

2021 ◽  
pp. 26-28
Author(s):  
Shruti Solanki ◽  
Palak Lakhani ◽  
Jaimin Patel ◽  
Jigna Upadhyay
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Tertiary Care ◽  
Medical Science ◽  
Large Cell ◽  
Punch Biopsy ◽  
Common Finding ◽  
Cervical Lesions ◽  
Keratinizing Squamous Cell Carcinoma

Aim:To find out various cervical lesions with histopathological correlation and age. Method: This was a prospective study conducted in pathology department of a tertiary care teaching hospital over a period of two years. A total of 255 women were included in this study. All cases of gynecological lesions at Histopathology department of Gujarat adani institute of medical science,Bhuj for 2 years starting from May 2019 to May 2021 were retrieved.The demographic information such as nature of specimen which include Punch biopsy(21 cases) and total abdominal hysterectomy(234 cases) were extracted. Results: The most common finding was Nonspecific chronic cervicitis in 159 patients(62.35%) followed by normal stratified squamous epithelium was found in 42 patients(16.47%) and Papillary endocervicitis were 14 patients(5.49%).In benign tumor or tumor-like lesion,Cervical metaplasia were 12 cases(4.70%),Nabothian cyst were 6 cases(2.35%) and Endocervical polyp were 2 cases(0.78%). The premalignant cervical lesions were only 4 cases(1.56%). The malignant cervical lesions were Large cell non-keratinizing squamous cell carcinoma were 7 cases(2.74%), Large cell keratinizing squamous cell carcinoma were 4 cases(1.56%),Squamous cell carcinoma NOS were 3 cases(1.17%) ,Poorly differentiated carcinoma was only one case(0.39%),Basaloid squamous cell carcinoma was only one case(0.39%). Conclusion: Cancer cervix is an ideal malignancy for screening,can be detected early and the premalignant lesion can be treated to prevent the progress to invasive disease. Hence all women with unhealthy cervix should be subjected to punch biopsy to detect early cases of cancer & carcinoma in situ. Most common cervical lesion is Squamous Cell carcinoma and most common age group is 40-49 years.

An immunohistochemical analysis of lymphocytic infiltrations in canine skin cancers

2017 ◽  
Vol 20 (1) ◽  
pp. 141-147 ◽  
Author(s):  
J.A. Madej ◽  
J.P. Madej ◽  
S. Dzimira ◽  
M. Nowak
Keyword(s):  
Squamous Cell Carcinoma ◽  
Skin Cancer ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Immunohistochemical Analysis ◽  
Lymphocytic Infiltration ◽  
Sebaceous Gland Carcinoma ◽  
Ki 67 ◽  
Inflammatory Infiltration ◽  
Keratinizing Squamous Cell Carcinoma

Abstract Lymphocytic infiltrations located in the extracellular matrix often accompany canine skin cancer. They can be characterised as an inflammatory infiltration and/or a second tumour - lymphoma. The aim of this study was an immunohistochemical analysis of a lymphocytic infiltration which accompanies spontaneous skin cancer. Twenty basal cell carcinoma, 20 non-keratinizing squamous cell carcinoma, 20 keratinizing squamous cell carcinoma and 8 sebaceous gland carcinoma samples which were accompanied by a lymphocytic infiltration and/or secondary lymphatic follicles were verified histopathologically. The expression of bcl-2, CD3, CD79α, Ki-67, MCM-3 and MCM-7 in the lymphocytic infiltration was evaluated. Four types of lymphocytic infiltrations were found: I - diffuse bcl-2+, II - diffuse bcl-2-, III - follicular bcl-2+/- where the centre was bcl-2-, and the marginal zone of the follicles and the extrafollicular area were bcl-2+ and IV - aggregated bcl-2+, where the centre and periphery were bcl-2+. The I and IV type corresponds to lymphoma, II type is non-neoplastic immune response and III type suggest reactive follicular hyperplasia. The proliferation of lymphocytes which demonstrated the expression of neoplastic markers (I and IV), suggests preneoplastic phase (pseudolymphoma) or lymphoma - the second independent tumour. A high proliferative index of the follicular blc-2+/- follicular infiltration indicates an increased immunological response of the host against skin cancer.

scholarly journals p16-negative warty carcinoma of the uterine cervix with superficial invasion to the endometrium: A case report focusing on diagnostic pitfalls

2017 ◽  
Vol 4 (3) ◽  
pp. 12
Author(s):  
Hyun-Jung Kim ◽  
Chul Min Lee
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Uterine Cervix ◽  
Low Risk ◽  
Proliferation Index ◽  
Invasive Squamous Cell Carcinoma ◽  
Ki 67 ◽  
Hpv Dna ◽  
Diagnostic Pitfalls

Warty carcinoma of the uterine cervix is a very rare and specific variant of invasive squamous cell carcinoma, usually described as a hybrid of the features of both condyloma acuminatum and invasive squamous cell carcinoma. The diagnostic pitfalls of this lesion are: 1) the bland appearance of cytomorphology, 2) distinct koilocytotic atypia, and 3) p16 negativity. A case of warty carcinoma with a fungating mass on the uterine cervix of a 75-year-old woman is presented. The original diagnosis by punch biopsy was atypical squamous cells, undetermined for malignancy, adjunct with p16 negativity. The subsequent radical hysterectomy showed a protruding mass (5.7 cm in horizontal dimension) of the uterine cervix. Light microscopy revealed a protruding mass composed of exophytic papillae and inverted nests along the endocervical glands. There were multiple micro-invasive foci (< 1 mm) of tongue like projections at the nest base and slight involvement of the upper vagina and endometrial surface. The p16 immunohistochemical staining yielded negative results. The results of the HPV DNA chip test from paraffin-embedded tissue were 6 (low risk, +++) and 42 (low risk, +). The Ki-67 proliferation index was approximately 20%. The invasive foci and patient’s age were the determining differential factors for malignancy rather than benign condylomatous lesion.

scholarly journals Adenosquamous carcinoma of the uterine cervix displaying tumor-associated tissue eosinophilia

2019 ◽  
Vol 7 ◽  
pp. 2050313X1982823
Author(s):  
Nozomu Kurose ◽  
Seiya Mizuguchi ◽  
Yoshiiku Ohkanemasa ◽  
Manabu Yamashita ◽  
Mariko Nakano ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Tumor Cells ◽  
Squamous Cell ◽  
Uterine Cervix ◽  
Adenosquamous Carcinoma ◽  
Tissue Eosinophilia ◽  
Tumor Tissues ◽  
Keratinizing Squamous Cell Carcinoma ◽  
Endometrial Cytology

Background: Tumor-associated tissue eosinophilia is defined as an inflammatory response with the marked infiltration of eosinophils within tumor tissues. Tumor-associated tissue eosinophilia has been reported in various organs; however, no studies have examined the detailed cytopathological findings of tumor-associated tissue eosinophilia. Case Presentation: A 49-year-old woman presented with lower abdominal and back pain that had started 1 month earlier. A cervical biopsy revealed a diagnosis of non-keratinizing squamous cell carcinoma. A mildly increased number of eosinophils was observed in both cervical cytology and a biopsy. On pelvic computed tomography, a tumor mass measuring up to 5.5 cm in the largest diameter was seen in the uterine cervix. After 1 month, endometrial cytology was performed, and non-keratinizing squamous cell carcinoma together with normal endometrial glands was obtained in a background of marked eosinophil numbers. Tumor cells in an irregular-shaped solid nest had variable-sized hyperchromatic nuclei and light-green-stained cytoplasm. The number of eosinophils was obviously increased. Considering the possibility of tumor-associated tissue eosinophilia, we evaluated a peripheral blood sample and confirmed an increased number of eosinophils. Radical hysterectomy was performed, and the final pathological diagnosis was adenosquamous carcinoma. Although the number of eosinophils decreased after surgery, it increased again at the time of recurrence 1 year later. Chemo-irradiation was performed, but the patient died 1 year and 8 months after the operation. Conclusion: Cytopathologists should consider the presence of tumor-associated tissue eosinophilia by focusing on not only tumor cells but also the markedly eosinophilic background. The eosinophil count might be a useful marker of the disease activity.

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