Identification TIMP-1 and TIMP-2 in Human Radicular Dentine - In Vitro Study
<strong>Aim</strong>: To identify TIMP – 1 and TIMP – 2 in human radicular dentine using confocal laser scanning microscopy. <strong>Materials and Methods</strong>: Thirty freshly extracted non carious human single rooted pre molars were obtained and stored in isotonic saline at -70°C prior to use. All the teeth were decoronated at the CEJ using a diamond. Teeth were divided into 2 groups (Group 1: TIMP-1 analysis n = 15; Group 2: TIMP-2 analysis n = 15). Teeth were sectioned using a hard tissue microtome, mounted and viewed under confocal laser scanning microscopy. <strong>Results</strong>: TIMP-1 and TIMP-2 were detected in radicular dentine and were seen to be distributed more towards the inner dentine layer closer to the pulp. <strong>Conclusion</strong>: Due to a shorter half life of TIMP-1 and 2 as compared to the MMP, there is a need to use MMP inhibitors prior to obturation of the root canal.