scholarly journals c-myc oncogene gene dosage, serum CEA and CA-15.3 antigen levels, and cellular DNA values in relation to ex vivo chemosensitivity of primary human breast cancer.

2000 ◽  
Vol 47 (1) ◽  
pp. 149-156 ◽  
Author(s):  
B Falkiewicz ◽  
C M Schlotter ◽  
U Bosse ◽  
K Bielawski ◽  
U Vogt
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Ex Vivo ◽  
Drug Combinations ◽  
Human Breast ◽  
Ca 15.3 ◽  
Myc Oncogene ◽  
Dna Values ◽  
Serum Cea ◽  
Primary Human Breast

A pilot study on relationships of selected molecular factors (c-myc oncogene average gene copy numbers (AGCN); serum CEA and CA 15.3 antigen levels; tumor cells' DNA values), to the ex vivo chemosensitivity of primary female human breast cancer in a modified adenosine triphosphate cell viability chemosensitivity assay (ATP-CVA), was performed. Four drug combinations were tested. A group of 75 cases of female primary breast cancer was assessed. Numerous correlations were found among molecular factors tested but none, with the exception of tumor grading, of these reflected ex vivo chemosensitivity of tumors tested. The results suggest that the parameters tested may not be important factors related to adjuvant chemoresponsiveness of primary human breast cancer to tested drug combinations.

Steroid hormone receptor activity of primary human breast cancer and pattern of first metastasis

1991 ◽  
Vol 18 (1) ◽  
pp. 27-32 ◽  
Author(s):  
P. G. Koenders ◽  
L. V. A. M. Beex ◽  
R. Langens ◽  
P. W. C. Kloppenborg ◽  
A. G. H. Smals ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Hormone Receptor ◽  
Human Breast Cancer ◽  
Steroid Hormone ◽  
Receptor Activity ◽  
Steroid Hormone Receptor ◽  
Human Breast ◽  
Primary Human Breast

Effects of Alternating Chemotherapy with Two Non-Cross-Resistant Drug Combinations on a Human Breast Cancer Xenografted into Nude Mice

2015 ◽  
pp. 258-262
Author(s):  
Masahide Fujita ◽  
Fumiko Fujita ◽  
Yasuo Sakamoto ◽  
Hiizu Inaba ◽  
Tetsuo Taguchi
Keyword(s):  
Breast Cancer ◽  
Nude Mice ◽  
Human Breast Cancer ◽  
Drug Combinations ◽  
Human Breast ◽  
Alternating Chemotherapy

scholarly journals Establishment of primary human breast cancer cell lines using “pulsed hypoxia” method and development of metastatic tumor model in immunodeficient mice

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Anna A. Nushtaeva ◽  
Anastasia A. Karpushina ◽  
Mikhail S. Ermakov ◽  
Ludmila F. Gulyaeva ◽  
Alexey V. Gerasimov ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Breast Cancer Cell ◽  
Human Breast Cancer ◽  
Tumor Model ◽  
Metastatic Tumor ◽  
Human Breast Cancer Cell ◽  
Breast Cancer Cell Lines ◽  
Human Breast ◽  
Immunodeficient Mice ◽  
Primary Human Breast

Assessment of early docetaxel response in an experimental model of human breast cancer using DCE-MRI, ex vivo HR MAS, and in vivo 1 H MRS

2009 ◽  
Vol 23 (1) ◽  
pp. 56-65 ◽  
Author(s):  
Line R. Jensen ◽  
Else M. Huuse ◽  
Tone F. Bathen ◽  
Pål E. Goa ◽  
Anna M. Bofin ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Experimental Model ◽  
Human Breast Cancer ◽  
Ex Vivo ◽  
Human Breast ◽  
Dce Mri

Relation between ER and EGFR in primary human breast cancer reports at variance

1990 ◽  
Vol 26 (2) ◽  
pp. 183
Author(s):  
P.G. Koendore ◽  
L.V.A.M. Beex ◽  
J.A. Foekens ◽  
Th.J. Benraad
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Human Breast ◽  
Primary Human Breast

scholarly journals Characterization of organoid cultured human breast cancer

2019 ◽  
Vol 21 (1) ◽  
Author(s):  
Nadine Goldhammer ◽  
Jiyoung Kim ◽  
Vera Timmermans-Wielenga ◽  
Ole William Petersen
Keyword(s):  
Breast Cancer ◽  
Primary Breast Cancer ◽  
Human Breast Cancer ◽  
Breast Cancers ◽  
Basal Cells ◽  
Human Breast ◽  
Contemporary Culture ◽  
Mucin 1 ◽  
Organoid Cultures ◽  
Primary Human Breast

AbstractOrganoid cultures are increasingly used to model human cancers experimentally with a view to tailoring personalized medicine and predicting drug responses. Breast cancer is no exception, but in particular, primary breast cancer poses some inherent difficulties due to the frequent presence of residual non-malignant cells in the biopsies. We originally developed an assay for the distinction between malignant and non-malignant structures in primary breast cancer organoid cultures (Petersen et al., Proc Natl Acad Sci (USA) 89(19):9064–8, 1992). Here, we apply this assay to assess the frequency of normal-like organoids in primary breast carcinoma cultures and the cellular composition as a consequence of passaging. We find that in consecutively collected samples of primary human breast cancers, residual non-malignant tissues were observed histologically in five out of ten biopsies. Based on relevant morphogenesis and correct polarization as recorded by expression in luminal epithelial cells of mucin 1 (Muc1), occludin, and keratin 19 (K19) and expression in basal cells of integrin β4, p63, and K14, non-malignant organoids were present in all primary human breast cancer-derived cultures. Furthermore, passaging in a contemporary culture medium was in favor of the selective expansion of basal-like cells. We conclude that organoid cultures of human breast cancers are most representative of the tissue origin in primary culture.

scholarly journals Antitumoral Effect of Laurinterol on 3D Culture of Breast Cancer Explants

Marine Drugs ◽  
2019 ◽  
Vol 17 (4) ◽  
pp. 201 ◽  
Author(s):  
Sara García-Davis ◽  
Ezequiel Viveros-Valdez ◽  
Ana Díaz-Marrero ◽  
José Fernández ◽  
Daniel Valencia-Mercado ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Crude Extract ◽  
Human Breast Cancer ◽  
Ex Vivo ◽  
3D Culture ◽  
Human Tumor ◽  
Individual Response ◽  
Human Breast ◽  
Antitumoral Effect ◽  
Wide Range

Macroalgae represent an important source of bioactive compounds with a wide range of biotechnological applications. Overall, the discovery of effective cytotoxic compounds with pharmaceutical potential is a significant challenge, mostly because they are scarce in nature or their total synthesis is not efficient, while the bioprospecting models currently used do not predict clinical responses. Given this context, we used three-dimensional (3D) cultures of human breast cancer explants to evaluate the antitumoral effect of laurinterol, the major compound of an ethanolic extract of Laurencia johnstonii. To this end, we evaluated the metabolic and histopathological effects of the crude extract of L. johnstonii and laurinterol on Vero and MCF-7 cells, in addition to breast cancer explants. We observed a dose-dependent inhibition of the metabolic activity, as well as morphologic and nuclear changes characteristic of apoptosis. On the other hand, a reduced metabolic viability and marked necrosis areas were observed in breast cancer explants incubated with the crude extract, while explants treated with laurinterol exhibited a heterogeneous response which was associated with the individual response of each human tumor sample. This study supports the cytotoxic and antitumoral effects of laurinterol in in vitro cell cultures and in ex vivo organotypic cultures of human breast cancer explants.

Prognostic Relevance of Microvessel Density (MVD) in primary human breast cancer and lymph node metastase

1997 ◽  
Vol 33 ◽  
pp. S194
Author(s):  
A. Grothey ◽  
A. Hasenburg ◽  
S Philippou ◽  
R. Voigtmann ◽  
K Quakernack
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Microvessel Density ◽  
Human Breast Cancer ◽  
Human Breast ◽  
Prognostic Relevance ◽  
Primary Human Breast
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