scholarly journals Activation of MAP kinase pathways in Galleria mellonella infected with Bacillus thuringiensis.

2014 ◽  
Vol 61 (1) ◽  
Author(s):  
Iwona Wojda ◽  
Konrad Koperwas ◽  
Teresa Jakubowicz
Keyword(s):  
Heat Shock ◽  
Bacillus Thuringiensis ◽  
Map Kinase ◽  
Map Kinases ◽  
Galleria Mellonella ◽  
Fat Body ◽  
Greater Wax Moth ◽  
Fat Bodies ◽  
Map Kinase Pathways ◽  
Wax Moth

We followed changes in the level of phospho-MAP kinases in the greater wax moth Galleria mellonella after infection with Bacillus thuringiensis. We observed an enhanced level of phosphorylated p38 and JNK in fat bodies of the infected larvae. In hemocytes, injection of B. thuringiensis caused the highest increase in phospho-JNK, however, all pathways were activated after aseptic injection. We report that Galleria mellonella larvae exposed to heat shock before infection showed an enhanced level of phosphorylated JNK in fat body. This finding is relevant in the light of our previous reports, which submit evidence that pre-shocked animals are more resistant to infection.

Hormonal regulation of the expression of two storage proteins in the larval fat body of the greater wax moth (Galleria mellonella)

2003 ◽  
Vol 49 (6) ◽  
pp. 551-559 ◽  
Author(s):  
Jakub Godlewski ◽  
Barbara Kłudkiewicz ◽  
Krystyna Grzelak ◽  
Małgorzata Beręsewicz ◽  
Bronisław Cymborowski
Keyword(s):  
Hormonal Regulation ◽  
Galleria Mellonella ◽  
Storage Proteins ◽  
Fat Body ◽  
Greater Wax Moth ◽  
Wax Moth

scholarly journals The effect of cold shock on the immune response of the greater wax moth Galleria mellonella after infection with entomopathogenic bacteria Bacillus thuringiensis / Wpływ szoku zimna na odpowiedź immunologiczną barciaka większego Galleria mellonella po zakażeniu bakterią entomopatogenną Bacillus thuringiensis

2015 ◽  
Vol 69 (2) ◽  
Author(s):  
Iwona Wojda ◽  
Paulina Taszłow ◽  
Teresa Jakubowicz
Keyword(s):  
Immune Response ◽  
Bacillus Thuringiensis ◽  
Cold Shock ◽  
Galleria Mellonella ◽  
Multifunctional Protein ◽  
Entomopathogenic Bacteria ◽  
Greater Wax Moth ◽  
Short Period ◽  
Defence Proteins ◽  
Wax Moth

AbstractInsect immune system consists of only innate mechanisms relied on cellular and humoral branches. Many defence proteins and peptides exist or appear in response to infection in insect’s hemolymph. The interaction between the infected host and the entomopathogen occurs in the conditions of external environment. In this work the greater wax moth larvae of Galleria mellonella were subjected to a temperature of 120C for a short period of time, directly before infection with entomopathogenic bacteria Bacillus thuringiensis. It appeared that the induction of the immune response was higher in cold-shocked animals than in larvae permanently reared at the optimal temperature of 28 0C. This enhanced immune response was manifested as higher antibacterial and lysozyme-type activity detected in full hemolymph, and as a higher level of peptides of molecular weight below 10 kDa having antibacterial activity. Moreover, other changes in the contents of proteins in the hemolymph were observed. These changes concerned inter alia apolipophorin III, the multifunctional protein of immune significance. Its level was higher in the hemolymph of animals pre-exposed to cold shock than in nonshocked, infected ones. Altogether our results indicate that the interdependence mechanisms occur between cold shock and the immune response.

scholarly journals Isolation of Bacillus thuringiensis and its Insecticidal Effect against Galleria mellonella

2020 ◽  
pp. 96-102
Author(s):  
Jyoti Limbu ◽  
Bijay Kumar Shrestha ◽  
Jenish Shakya ◽  
Sabin Bahadur Khatri ◽  
Hemanta Khanal
Keyword(s):  
Bacillus Thuringiensis ◽  
Insecticidal Activity ◽  
Galleria Mellonella ◽  
Geographical Location ◽  
Insecticidal Effect ◽  
Greater Wax Moth ◽  
Effective Level ◽  
And Control ◽  
Geographical Locations ◽  
Wax Moth

Bacillus thuringiensis (Bt) synthesize a large diversity of crystal proteins (Cry and Cyt) during sporulation which exhibit insecticidal activity against insects and protozoa. The main aim of this study was to isolate Bacillus thuringiensis and study its insecticidal effect against Galleria mellonella. Soil samples from four different geographical locations of Koshi Zone viz. Itahari, Tarhara, Dharan and Vedetar of Eastern Nepal were collected. The isolation of Bt was done by acetate selection method. The insect bioassay of Bt isolates were performed against greater wax moth (G. mellonella) by feeding the third instar larvae by extracted crystal spores with three different concentrations. The overall distribution of Bt from the study sample was found to be 30% (30/100). Bt was isolated from all four geographical location with higher incidence; 9 (36%) in Tarhara region followed by Dharan (32%), Itahari (28%) and Vedetar (24%). However, the incidence of Bt with potent insecticidal activity against G. mellonella was reported to be 4% (4/100). The insecticidal activity of isolated Bt between test and control groups was found to be statistically significant (p<0.05). LC50 value of Bt from Tarhara (Tar1) was 388.29μg/mL, Dharan; Drn8 and Drn1 was 416.20μg/mL and 463.15μg/mL respectively and from Vedetar (Vd5) was 476.63μg/mL. In overall study the Bt isolated from Tarhara (Tar1) region exhibited greater incidence, Bt index, efficacy and effective level of LC50 against greater wax moth. Native Bt strains isolated from soil of Eastern Nepal possess effective insecticidal activity and hence can used as biocontrol agent in controlling honeycomb pest like G. mellonella.

Fat on Plastic: metabolic consequences of an LDPE diet in the fat body of the greater wax moth larvae (Galleria mellonella)

2021 ◽  
pp. 127862
Author(s):  
Bryan J. Cassone ◽  
Harald C. Grove ◽  
Nicholas Kurchaba ◽  
Paola Geronimo ◽  
Christophe MR. LeMoine
Keyword(s):  
Galleria Mellonella ◽  
Fat Body ◽  
Greater Wax Moth ◽  
Wax Moth
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