scholarly journals Enhanced Prokaryotic Expression of Dengue Virus Envelope Protein

2013 ◽  
Vol 16 (4) ◽  
pp. 609 ◽  
Author(s):  
Advaita Ganguly ◽  
Ravindra B. Malabadi ◽  
Dipankar Das ◽  
Mavanur R. Suresh ◽  
Hoon H Sunwoo
Keyword(s):  
Dengue Virus ◽  
Envelope Protein ◽  
Vaccine Development ◽  
Virus Envelope ◽  
E Coli ◽  
C Terminus ◽  
Virus Envelope Protein ◽  
Biological Functionality ◽  
Dengue Diagnostics

Purpose. To highlight the expression and purification of the recombinant dengue virus type-1 antigen exploiting the codon optimized full length envelope for increased yield in E. coli. Methods. A 6x His tag was inserted at the C terminus to facilitate purification. The purified protein was recognized in Western blot by Monoclonal antibody specific for the tag. The in vitro refolded recombinant protein was used to immunize mice for the development of hybridomas and also analyzed for its biological functionality with heparan sulfate binding assay. Results. The polyclonal anti-sera from the immunized mice were found to recognize the envelope protein thereby establishing the immunogenicity of the protein. Conclusion. The purified envelope protein could potentially be used towards dengue diagnostics and vaccine development efforts. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

scholarly journals Dengue Virus Envelope Protein Domain III Induces Nlrp3 Inflammasome-Dependent NETosis-Mediated Inflammation in Mice

2021 ◽  
Vol 12 ◽  
Author(s):  
Te-Sheng Lien ◽  
Der-Shan Sun ◽  
Shih-Che Hung ◽  
Wen-Sheng Wu ◽  
Hsin-Hou Chang
Keyword(s):  
Dengue Virus ◽  
Nlrp3 Inflammasome ◽  
Envelope Protein ◽  
Protein Domain ◽  
Severe Dengue ◽  
Cytokine Storm ◽  
Virus Envelope ◽  
Virus Envelope Protein ◽  
Domain Iii

Abnormal immune responses and cytokine storm are involved in the development of severe dengue, a life-threatening disease with high mortality. Dengue virus-induced neutrophil NETosis response is associated with cytokine storm; while the role of viral factors on the elicitation of excessive inflammation mains unclear. Here we found that treatments of dengue virus envelope protein domain III (EIII), cellular binding moiety of virion, is sufficient to induce neutrophil NETosis processes in vitro and in vivo. Challenges of EIII in inflammasome Nlrp3−/− and Casp1−/− mutant mice resulted in less inflammation and NETosis responses, as compared to the wild type controls. Blockages of EIII-neutrophil interaction using cell-binding competitive inhibitor or selective Nlrp3 inflammasome inhibitors OLT1177 and Z-WHED-FMK can suppress EIII-induced NETosis response. These results collectively suggest that Nlrp3 inflammsome is a molecular target for treating dengue-elicited inflammatory pathogenesis.

scholarly journals Production of tetravalent dengue virus envelope protein domain III based antigens in lettuce chloroplasts and immunologic analysis for future oral vaccine development

2019 ◽  
Vol 17 (7) ◽  
pp. 1408-1417 ◽  
Author(s):  
André Eerde ◽  
Johanna Gottschamel ◽  
Ralph Bock ◽  
Kristine Eraker Aasland Hansen ◽  
Hetron Mweemba Munang'andu ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Envelope Protein ◽  
Vaccine Development ◽  
Oral Vaccine ◽  
Protein Domain ◽  
Virus Envelope ◽  
Virus Envelope Protein ◽  
Domain Iii ◽  
Immunologic Analysis

Epitopes based drug design for dengue virus envelope protein: A computational approach

2017 ◽  
Vol 71 ◽  
pp. 152-160 ◽  
Author(s):  
Abdul Wadood ◽  
Aamir Mehmood ◽  
Huma Khan ◽  
Muhammad Ilyas ◽  
Ayaz Ahmad ◽  
...  
Keyword(s):  
Drug Design ◽  
Dengue Virus ◽  
Envelope Protein ◽  
Protein A ◽  
Computational Approach ◽  
Virus Envelope ◽  
Virus Envelope Protein

Dengue virus envelope protein domain III-elicited antibodies mediate cross-protection against Zika virus in a mouse model

2020 ◽  
Vol 278 ◽  
pp. 197882
Author(s):  
Yongchao Zhou ◽  
Dong Chen ◽  
Lan Yang ◽  
Weiwei Zou ◽  
Zhiliang Duan ◽  
...  
Keyword(s):  
Mouse Model ◽  
Dengue Virus ◽  
Zika Virus ◽  
Envelope Protein ◽  
Cross Protection ◽  
Protein Domain ◽  
Virus Envelope ◽  
Virus Envelope Protein ◽  
Domain Iii

Transient expression of dengue virus envelope protein in a plant system

2010 ◽  
Vol 150 ◽  
pp. 484-484
Author(s):  
C. Martinez ◽  
A.M Giulietti ◽  
J. Rodriguez Talou ◽  
H. Mason
Keyword(s):  
Dengue Virus ◽  
Transient Expression ◽  
Envelope Protein ◽  
Virus Envelope ◽  
Plant System ◽  
Virus Envelope Protein

scholarly journals Probing the Interaction of Dengue Virus Envelope Protein with Heparin:  Assessment of Glycosaminoglycan-Derived Inhibitors

2001 ◽  
Vol 44 (13) ◽  
pp. 2178-2187 ◽  
Author(s):  
Rory M. Marks ◽  
Hong Lu ◽  
Renuka Sundaresan ◽  
Toshihiko Toida ◽  
Atsushi Suzuki ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Envelope Protein ◽  
Virus Envelope ◽  
Virus Envelope Protein
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