Prevalence of Occult Hepatitis B Infection among HBsAg Negative Blood Donors in Golestan Province

2019 ◽  
Author(s):  
Rana Tabar Asad Laleh ◽  
Zohreh Sharifi ◽  
Akbar Pourfathollah ◽  
Shahram Samei
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Blood Donors ◽  
Hbv Dna ◽  
High Rate ◽  
Analytical Sensitivity ◽  
Occult Hepatitis ◽  
B Virus ◽  
Occult Hepatitis B ◽  
Golestan Province

Background and Aims: Occult hepatitis B virus infection (OBI) is known as an important source of hepatitis B virus (HBV) infection. It is categorized as Hepatitis B surface antigen (HBsAg) not being present and low DNA viral load in serum. In this study, an attempt was made to investigate the outbreak of anti-HBc and OBI among the HBsAg-negative donors in Golestan province. Materials and Methods: The present cross-sectional experiment was conducted on 3500 voluntary blood donors in Golestan province to examine the presence of human immunodeficiency viruses Ag-Ab, HBsAg, and hepatitis C virus Ab. Then, samples with negative results for the mentioned tests were screened for total HBc antibody (IgM-IgG) through ELISA technique. Afterward, HBV-DNA extraction and R-T PCR assay were conducted for all HBsAg negative samples by using Real ART HBV LC PCR kit on a Light Cycler instrument. Results: The study participants included 3255 (93%) male and 245 (7%) female. In general, 385 (11%) out of 3500 samples were anti-HBc positive. HBV-DNA results for every sample with either positive or negative anti-HBc were found to be negative. Conclusions: As the area under study has a high rate of anti-HBc outbreak (11%) without the presence of HBV-DNA, anti-HBc screening can cause blood donor deferrals and limit blood supply; therefore, the HBsAg test with high analytical sensitivity is recommended for HBV screening in this area. Regarding the cost analyses and also the status of HBV endemicity, HBsAg test along with ID-NAT is preferable, if possible, for improving blood safety.

scholarly journals Hepatitis B virus DNA in Blood Donors Positive of Anti-Hepatitis B Core Antibodies and Negative for Surface Antigen in Hawler Major Blood Bank, Kurdistan Region, Iraq

2018 ◽  
Vol 60 (1) ◽  
pp. 57-61
Author(s):  
Rasha N. Hassan ◽  
Ali H. Hussain
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Real Time ◽  
Real Time Pcr ◽  
Blood Donors ◽  
Hbv Dna ◽  
Occult Hepatitis ◽  
B Virus ◽  
Occult Hepatitis B ◽  
Hbs Ag

Background: Occult Hepatitis B virus infection (OBI) among blood donors is an important medical concern.Objectives: This study was done to detect the presence of occult hepatitis B virus infections among blood donors with negative hepatitis B surface antigen and positive total anti-hepatitis B core antibodies in Hawler Major Blood Bank in Hawler city/Kurdistan Region of Iraq.Methods: A total number of 12,185 blood donors in Hawler Major Blood Bank were screened for HBsAg and total anti-HBcAb using ELISA technique, and then positive results were retested by confirmatory technique by Chemiluminescence assay. All HBsAg-/HBcAb+ were selected as the study group; HBV DNA was tested among HBsAg-/HBcAb+ by conventional PCR and Real time-PCR. Clinical and demographic data of study group were recorded.Results: Among the 12,185 blood donors, HBsAg was positive in 27 (0.22%) donors using Chemiluminescence assay, the frequency of HBs Ag -/ HBc Ab+ was 276 (2.27%), and then the total prevalence of HBV infection in all blood donors was 2.49%. Among the 276 HBs Ag-/HBcAb+, occult hepatitis B virus infection (OBI) was positive in 39.1% (108/276) using conventional PCR and Real time-PCR techniques, while the prevalence among all blood donors (n=12,185) was 0.09%. Testing of HBV-DNA in HBs Ag -/ HBc Ab+ group for OBI was done by qualitative PCR (positive HBV-DNA=102/276) or by quantitative Real time-PCR (positive HBV-DNA=108/276).Conclusions: The OBI is frequently detected among blood donors in Hawler city especially those have HBsAg-/HBcAb+, and the total anti-HBcAb is an essential serological marker for screening HBV among blood donors. The risk factors for developing OBI among blood donors should be elucidated.   الحامض النووي  الريبوزي منقوص الأكسجين للفايروس الكبدي نوع ب في دم المتبرعين والموجب لأضداد اللب للفايروس وسالب للستضد السطحي للفايروس في مصرف الدم الرئيسي في اربيل/كردستان العراق  رشا نزار حسن علي حاتم حسين نظرة اولية: ان تواجد الاصابة  بالفايروس الكبدي نوع ب لدى المتبرعين بالدم يعتبر من الحالات الطبية التي يجب الانتباه اليها. الاهداف: تم اجراء هذه الدراسة على المتبرعين بالدم في مصرف الدم الرئيسي في مدينة اربيل/كردستان العراق , لغرض التحري عن وجود الاصابات الكبدية الفايروسية نوع ب المخفية لدى المتبرعين بالدم والذين يكون المستضاد السطحي الفايروسي الكبدي نوع ب (HBsAg) سالبا لديهم ويكون المضاد اللبي الكلي لنفس الفايروس(anti-HBcAb) موجبا. طريقة البحث: تم فحص اثنا عشر الفا ومائه وخمس وثمانين متبرع بالدم في مصرف الدم الرئيسي في مدينة اربيل لغرض الكشف عن (HBsAg) و  (anti-HBcAb) وان النتائج الموجبة تم اعادة فحصها بطرق فحص تأكيدية. ان كل المتبرعين ذوو النتائج (السالبة HBsAg ) / و (الموجبة anti-HBcAb) تم انتقئهم كمجموعة الدراسة وتم فحص وجود الحامض النووي  الريبوزي منقوص الأكسجين للفايروس الكبدي نوع ب لديهم في الدم بطريقتين: طريقة تفاعل البوليميراز المتسلسل التقليدية وطريقة تفاعل البوليميراز المتسلسل اللحظي. كما تم تسجيل البيانات الديموغرافية والسريرية لمجموعة الدراسة. النتائج:  لدى فحص المتبرعين البالغ عددهم  اثنا عشر الفا ومائه وخمس وثمانين متبرع تبين وجود سبعة وعشرون اصابة (0.02%)  بالمستضاد (HBsAg) اما عدد المتبرعين الذين ليس لديهم  / HBsAg والموجبة للمضاد anti-HBcAb  فقد كان 276 (2.27%) وقد تبين ان نسبة انتشار الخمج البدي الفايروسي نوع ب لدى المتبرعين كان 2.49%. عند فحص مجموعة الدراسة البالغ عددها 276 والذين كانت نتيجة فحصهم موجبة للمضاد /HBcAb,وسالبة للمستضاد HBsAg ,تبين وجود الخمج الفايروسي الكبدي نوع ب المخفي في 39.1% (108/276),  بينما كانت نسبة انتشار نفس الخمج لدى مجموع المتبرعين الكلي البالغ 12185 هو 0.09%. لقد كانت نتيجة فحص وجود الحامض النووي  الريبوزي منقوص الأكسجين للفايروس الكبدي نوع ب بالطريقة القليدية هي 102 متبرع من مجموعة الدراسة الباغة 276 اما النتيجة لنفس مجموعة الدراسة لنفس الحامض النووي بطريقة تفاعل البوليميراز المتسلسل اللحظي كانت 108. الاستنتاج: لقد بينت هذه الدراسة وجود وجود الخمج الفايروسي الكبدي نوع ب المخفي بصورة متكررة لدى المتبرعين بالدم في مدينة اربيل وخاصة الذين تكون حالتهم  موجبة للمضاد /HBcAb, وسالبة للمستضاد HBsAg كما بينت الدراسة ان فحص  المضاد اللبي للفايروس الكبدي نوع ب هو ضروري عند التحري عن وجود وجود الخمج الفايروسي الكبدي نوع ب لدى المتبرعين بالدم, كما انه من الضروري التقصي عن عوامل الخطورة التي تؤدي الى الاصابة بالخمج الفايروسي الكبدي نوع ب المخفي. الكلمات المفتاحية: المتبرعون بالدم, الخمج الفايروسي الكبدي نوع ب المخفي, مدينة اربيل, مصرف الدم, تفاعل البوليمراز المتسلسل التقليدي واللحظي

scholarly journals Occult Hepatitis B Virus Infection among HIV Positive Patients in Nigeria

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Oluyinka Oladele Opaleye ◽  
Adeolu Sunday Oluremi ◽  
Adetona Babatunde Atiba ◽  
Moses Olubusuyi Adewumi ◽  
Olatunji Victor Mabayoje ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Hbv Dna ◽  
Hiv Positive ◽  
Hepatitis B Virus Infection ◽  
Occult Hepatitis ◽  
B Virus ◽  
Occult Hepatitis B ◽  
Detectable Hbsag

HIV has been known to interfere with the natural history of hepatitis B virus (HBV) infection. In this study we investigate the prevalence of occult hepatitis B virus infection (OBI) among HIV-infected individuals in Nigeria. Overall, 1200 archived HIV positive samples were screened for detectable HBsAg using rapid technique, in Ikole Ekiti Specialist Hospital. The HBsAg negative samples were tested for HBsAg, anti-HBc, and anti-HCV by ELISA. Polymerase chain reaction was used for HBV DNA amplification and CD4 counts were analyzed by cytometry. Nine hundred and eighty of the HIV samples were HBsAg negative. HBV DNA was detected in 21/188 (11.2%) of patients without detectable HBsAg. CD4 count for the patients ranged from 2 to 2,140 cells/μL of blood (mean = 490 cells/μL of blood). HCV coinfection was detected only in 3/188 (1.6%) of the HIV-infected patients (P>0.05). Twenty-eight (29.2%) of the 96 HIV samples screened were positive for anti-HBc. Averagely the HBV viral load was <50 copies/mL in the OBI samples examined by quantitative PCR. The prevalence of OBI was significantly high among HIV-infected patients. These findings highlight the significance of nucleic acid testing in HBV diagnosis in HIV patients.

Occult Hepatitis B Virus Infection Among Blood Donors of North India

2007 ◽  
Vol 4 (4) ◽  
pp. 339-342
Author(s):  
R.N. Makroo ◽  
V. Raina ◽  
M. Chowdhry ◽  
M. Pathuppalli
Keyword(s):  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Blood Donors ◽  
North India ◽  
Hepatitis B Virus Infection ◽  
Occult Hepatitis ◽  
B Virus ◽  
Occult Hepatitis B

scholarly journals Occult hepatitis B virus infection among hemodialysis patients

2018 ◽  
Vol 91 (2) ◽  
Author(s):  
Rahil Nahid Samiei ◽  
Somayeh Shokri ◽  
Shahab Mahmoudvand ◽  
Manoochehr Makvandi ◽  
Heshmatollah Shahbazian ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Hbv Dna ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hepatitis B Infection ◽  
Nested Polymerase Chain Reaction ◽  
Occult Hepatitis ◽  
B Virus ◽  
The World ◽  
Occult Hepatitis B

Hepatitis B virus is a major public health impasse all over the world. Recently a new form of hepatitis B infection named Occult hepatitis B Infection (OBI) has appeared globally. The OBI is defined as the presence of HBV DNA in the liver and/or blood in the absence of detectable serum HBsAg with/without anti-HBc or anti-HBs. The prevalence of OBI has been reported in hemodialysis (HD) patients in different regions of the world. Thus, this study investigated the prevalence of OBI among HD patients. The cross-sectional study was carried out on 84 HD patients. These sera were checked for HBsAg, HBc-IgG assessment using Enzyme linked immunosorbent assay. The DNA was extracted from the sera samples and tested for HBVDNA detection using Nested Polymerase Chain Reaction (Nested PCR). The liver function tests including serum alanine aminotransferase and aspartate aminotransferase levels were carried out for all the HD individuals. 52/84(61.9%) of HD were males and 32/84 (38.1%) were females. The patient’s age ranged from 25 to 64 with a mean age of 52.4±15.2 years. HBsAg and HBc-IgG were detected in 1(1.1%) female. 2 (2.4%; a female and a male) patients were positive for HBsAg. 14/84 (16.7%; 6 female and 8 male) HD patients were positive for anti-HBc but negative for HBsAg, among them 4(28.6%; 2 female and 2 male) cases were positive for HBV DNA, indicating the presence of OBI in HD patients. Even distribution of OBI among the HD was found in 2(2.36%) male and 2(2.36%) female (P>.0.05). In the present study the moderate rate of 4.76% OBI has been observed in HD patients. The prevalence of seropositive OBI among the gender was 2(2.36%) male and 2(2.36%) female. The seronegative OBI have not been detected in the present study but requires further investigation. In this study the affliction of OBI in HD patients is not clear.

scholarly journals Erratum to: Occult hepatitis B in blood donors in Indonesia: altered antigenicity of the hepatitis B virus surface protein

2010 ◽  
Vol 4 (4) ◽  
pp. 788-788
Author(s):  
Meta D. Thedja ◽  
Martono Roni ◽  
Alida R. Harahap ◽  
Nurjati C. Siregar ◽  
Susan I. Ie ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Blood Donors ◽  
Surface Protein ◽  
Virus Surface ◽  
Occult Hepatitis ◽  
B Virus ◽  
Occult Hepatitis B

scholarly journals Prevalence of Occult Hepatitis B Virus Infections Among Kidney Transplant Patients in Khartoum State, Sudan

2020 ◽  
pp. 80-84
Author(s):  
Mashaer M Mustafa ◽  
Abdel Rahim M El Hussein ◽  
Isam M Elkhidir ◽  
Khalid A Enan
Keyword(s):  
Hepatitis B Virus ◽  
Renal Transplant ◽  
Hepatitis B ◽  
Hbv Dna ◽  
Competitive Elisa ◽  
Occult Hepatitis ◽  
Transplant Patients ◽  
B Virus ◽  
Occult Hepatitis B ◽  
Renal Transplant Patients

This study was carried out to detect occult hepatitis B virus (OHB) among renal transplant patients in Khartoum State, Sudan. Antigen capture enzyme linked immunosorbent assay (ELISA) competitive ELISA and polymerase chain reaction (PCR) were used to respectively detect hepatitis B surface antigen (HBsAg), ; Hepatitis B core antibody (HBcAb) and hepatitis B virus (HBV) DNA in 100 plasma samples collected from patients during the period from May to October 2018 . Out of the 100 patients sampled, 70 were males and 30 were females (age 15 to 65 years) and none of these patients showed signs of clinical hepatitis. The results showed that 3 out of the 100 samples were positive for HBsAg, and were subsequently excluded from the study. Out of the remaining HBsAg negative 97 samples, 39(40.2%) showed positive HBcAb and none (0%) tested positive to HBV DNA using competitive ELISA and PCR, respectively. These results indicated that more investigations including more patients from other transplant centers are needed to fully elucidate the situation of occult hepatitis B in renal transplant patients in Sudan.

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