scholarly journals Isolation and Characterization of Collagenase from Bacillus thuringiensis for Degrading Fish Skin Collagen of Cirata Reservoir Waste

2017 ◽  
Vol 2 (6) ◽  
pp. 172
Author(s):  
Emma Rochima ◽  
Rusky Intan Pratama ◽  
Yuli Andriani
Keyword(s):  
Bacillus Thuringiensis ◽  
Crude Extract ◽  
Protein Concentration ◽  
Clear Zone ◽  
Collagenase Activity ◽  
Isolation And Characterization ◽  
Skin Collagen ◽  
Optimum Ph ◽  
Optimum Production

The objective of this research was to isolate and characterize collagenase of Bacillus thuringiensis obtained from the collection of Aquatic Biotechnology Laboratory, Faculty of Fisheries and Marine Science, Padjadjaran University.  The substrate of collagen was derived from skin Tilapia waste of Cirata Reservoar. This study showed the presence of clear zone which is a sign of colagenolitik activity of B. thuringiensis. The optimum production time of collagenase was 24 hours of incubation. Collagenase of crude extract had collagenase activity of 0.181 units/ml with the protein concentration was 0.640 mg/ml. It was also found that the optimum temperature of collagenase derived from crude extract was 50° C and the optimum pH was 7-9. Keywords: collagenase; Bacillus thuringiensis;  skin, waste

Purification and partial amino acid sequence of thuricin S, a new anti-Listeriabacteriocin from Bacillus thuringiensis

2007 ◽  
Vol 53 (2) ◽  
pp. 284-290 ◽  
Author(s):  
Sonia Chehimi ◽  
François Delalande ◽  
Sophie Sablé ◽  
Mohamed-Rabeh Hajlaoui ◽  
Alain Van Dorsselaer ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Proteinase K ◽  
Terminal Sequence ◽  
Partial Amino Acid Sequence ◽  
Ph Values ◽  
Heat Stable ◽  
Isolation And Characterization ◽  
Terminal Amino ◽  
Edman Sequencing

We report the isolation and characterization of a new bacteriocin, thuricin S, produced by the Bacillus thuringiensis subsp. entomocidus HD198 strain. This antibacterial activity is sensitive to proteinase K, is heat-stable, and is stable at a variety of pH values (3–10.5). The monoisotopic mass of thuricin S purified by high perfomance liquid chromatography, as determined with mass spectrometry ESI-TOF-MS, is 3137.61 Da. Edman sequencing and NanoESI-MS/MS experiments provided the sequence of the 18 N-terminal amino acids. Interestingly, thuricin S has the same N-terminal sequence (DWTXWSXL) as bacthuricin F4 and thuricin 17, produced by B. thuringiensis strains BUPM4 and NEB17, respectively, and could therefore be classified as a new subclass IId bacteriocin.

scholarly journals Isolation and characterization of Bacillus thuringiensis strains active against Elasmopalpus lignosellus (Zeller, 1848) (Lepidoptera, Pyralidae)

2017 ◽  
Vol 39 (4) ◽  
pp. 417
Author(s):  
Janaina Zorzetti ◽  
Ana Paula Scaramal Ricietto ◽  
Fernanda Aparecida Pires Fazion ◽  
Ana Maria Meneguim ◽  
Pedro Manuel Oliveira Janeiro Neves ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Isolation And Characterization ◽  
Elasmopalpus Lignosellus ◽  
Lepidoptera Pyralidae

scholarly journals Isolation and characterization of Bacillus thuringiensis (Ernst Berliner) strains indigenous to agricultural soils of Mali

2015 ◽  
Vol 10 (28) ◽  
pp. 2748-2755 ◽  
Author(s):  
Kassogue Adounigna ◽  
Maiga Kadia ◽  
Traore Diakaridia ◽  
Hamadoun Dicko Amadou ◽  
Fane Rokiatou ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Agricultural Soils ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of Bacillus thuringiensis strains from different grain habitats in Turkey

2005 ◽  
Vol 21 (3) ◽  
pp. 285-292 ◽  
Author(s):  
Özgür Apaydin ◽  
A. Fazil Yenidünya ◽  
Şebnem Harsa ◽  
Hatice Güneş
Keyword(s):  
Bacillus Thuringiensis ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Lupeol from the Stem of Tapinanthus globiferus (A Rich.) and its Antimicrobial Assay

2020 ◽  
Vol 24 (6) ◽  
pp. 1015-1020
Author(s):  
V. Emaikwu ◽  
I.G. Ndukwe ◽  
R. Mohammed ◽  
O.R.A. Iyun ◽  
J.V. Anyam
Keyword(s):  
Spectral Analysis ◽  
Ethyl Acetate ◽  
Column Chromatography ◽  
Crude Extract ◽  
Solvent System ◽  
Pentacyclic Triterpenoid ◽  
Isolation And Characterization ◽  
Reported Data ◽  
Antimicrobial Assay

Lupeol, a pentacyclic triterpenoid, was isolated from hexane and ethyl acetate solvent system. In antiquity, the stem and leaf infusion of Tapinanthus globiferus has been used ethno-medicinally as a remedy for stomach ache, diarrhea, dysentery, and wounds. Lupeol isolation from this species was carried out by column chromatography after concentrating the crude extract using a rotary evaporator, and the structure was determined by analysis of the isolate by IR, 13CNMR, 1HNMR, HSQC, and HMBC spectral analysis as well as comparison with reported data. This is the first isolation of lupeol from the stem of this species. Keywords: Tapinanthus globiferus, Column chromatography, dysentery, Lupeol

Isolation and characterization of the large cyanogen bromide peptides from the α1- and α2-chains of pig skin collagen

FEBS Letters ◽  
1971 ◽  
Vol 16 (1) ◽  
pp. 63-67 ◽  
Author(s):  
W. Heinrich ◽  
P.M. Lange ◽  
T. Stirtz ◽  
C. Iancu ◽  
E. Heidemann
Keyword(s):  
Cyanogen Bromide ◽  
Pig Skin ◽  
Isolation And Characterization ◽  
Skin Collagen
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