scholarly journals Comparison of Effect of Piperine and Capsaicin with Tabata Exercise on Changes in Serum Nitric Oxide and Creatine Kinase of Kung Fu Boys

2021 ◽  
Author(s):  
Farah Nameni ◽  
Shahrzad Sadat Abbasabadi
Keyword(s):  
Nitric Oxide ◽  
Creatine Kinase ◽  
Oxidative Damage ◽  
Kinase Activity ◽  
Exercise Group ◽  
Creatine Kinase Activity ◽  
Kung Fu ◽  
Significant Difference ◽  
Bonferroni Test ◽  
Muscle Stress

Introduction: Tabata exercise programs can produce free radicals and muscle soreness and herbal supplements may be helpful as mediators in response to oxidative damage and muscle stress. Therefore, the purpose of this study was to compare the effects of these two supplements with Tabata exercise activity on nitric oxide and creatine kinase enzyme. Methods: The research was a quasi-experimental one and the participants were boys practicing Kung Fu. Among them, 33 boys (19-25 years old) were selected as a research sample by obtaining written consent and were randomly divided into three groups: Tabata exercise, Tabata exercise + capsaicin, Tabata exercise + piperine). During the eight weeks of piperine and capsaicin supplementation, all three groups participated in a Tabata training protocol. After the last session, blood sampling was taken in two basic stages immediately.The results were compared with one-way analysis of variance and Bonferroni test in the inferential section and SPSS version 16 software. Results: Creatine kinase activity in the two groups of supplement + exercise compared to the exercise group had a significant decrease (p = 0.004) and nitric oxide (p = 0.001) in the two groups of supplement + exercise compared to the exercise group had a significant increase. These changes were more pronounced in the Tabata exercise group with capsaicin supplementation. This significance was confirmed by Bonferroni test. There was no significant difference between the two groups of Tabata exercise and capsaicin and peprin consumption. Conclusion: Both piperine and capsaicin supplements decreased creatine kinase activity and increased nitric oxide. These two supplements were able to reduce some of the oxidative damage and muscle stress resulting from exercise by different mechanisms.

Observations on the Fluorometric Measurement of Creatine Kinase Activity

1968 ◽  
Vol 14 (7) ◽  
pp. 660-663 ◽  
Author(s):  
Sylvan M Sax ◽  
John J Moore
Keyword(s):  
Creatine Kinase ◽  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
High Background ◽  
Phosphatase Activities ◽  
Background Fluorescence ◽  
Significant Difference

Abstract Twenty patients’ serums have been analyzed by both the Sax-Moore (1) and Conn-Anido (2) creatine kinase methods. No significant difference in enzyme activity was found. In our hands, the Conn-Anido procedure, as originally described, gave variable results and high background fluorescence. The presence of high alkaline or acid phosphatase activities in specimens analyzed by the Sax-Moore procedure does not cause spurious elevations of creatine kinase.

Distribution of serum creatine kinase activity in young healthy persons

1999 ◽  
Vol 279 (1-2) ◽  
pp. 107-115 ◽  
Author(s):  
Eli I. Lev ◽  
Ilan Tur-Kaspa ◽  
Isaac Ashkenazy ◽  
Anat Reiner ◽  
David Faraggi ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
Serum Creatine Kinase ◽  
Healthy Persons

scholarly journals Ability of adenovirus 5 E1A proteins to suppress differentiation of BC3H1 myoblasts correlates with their binding to a 300 kDa cellular protein.

1992 ◽  
Vol 3 (10) ◽  
pp. 1107-1115 ◽  
Author(s):  
J S Mymryk ◽  
R W Lee ◽  
S T Bayley
Keyword(s):  
Creatine Kinase ◽  
Kinase Activity ◽  
Cellular Protein ◽  
Creatine Kinase Activity ◽  
Cellular Proteins ◽  
Deletion Mutants ◽  
Transcriptional Enhancers ◽  
Actin Mrna ◽  
Alpha Actin ◽  
Adenovirus 5

We have used deletion mutants to define the regions in Ad5 E1A proteins necessary to suppress differentiation of mouse BC3H1 myoblasts. We examined the differentiation of cells infected at a low multiplicity with viruses containing the E1A deletions and constructed so as to produce only the smaller of the two major E1A proteins. Only four of the mutant viruses containing deletions within the N-terminal 69 residues failed to suppress differentiation as judged by changes in morphology and in levels of muscle-specific alpha-actin mRNA and creatine kinase activity. The results were confirmed by analyses of lines of cells stably transfected with representative E1A mutants. The mouse cellular proteins to which mutant E1A proteins bound were identified by immunoprecipitating E1A proteins specifically from infected BC3H1 cells and by analyzing the precipitates on denaturing gels. Bands of proteins of 300, 130, 107, 105 (the retinoblastoma product), and 60 kDa (cyclin A) were distinguished. Failure to suppress differentiation correlated with loss of binding to the 300-kDa protein but not to any of the others. The regions of E1A defined in this way have been shown to be required for several other activities, including enhancer repression and transformation. One function of the 300-kDa protein appears to be to facilitate the action of transcriptional enhancers of differentiation-specific genes.

Skeletal muscle collagen content in humans after high-force eccentric contractions

2004 ◽  
Vol 97 (1) ◽  
pp. 197-203 ◽  
Author(s):  
Abigail L. Mackey ◽  
Alan E. Donnelly ◽  
Taina Turpeenniemi-Hujanen ◽  
Helen P. Roper
Keyword(s):  
Creatine Kinase ◽  
Kinase Activity ◽  
Staining Intensity ◽  
Creatine Kinase Activity ◽  
Serum Creatine Kinase ◽  
Muscle Contractions ◽  
Tissue Inhibitors Of Metalloproteinases ◽  
Eccentric Contractions ◽  
Knee Extensors ◽  
Type Iv

The purpose of this study was to investigate the effects of high-force eccentric muscle contractions on collagen remodeling and on circulating levels of matrix metalloproteinases (MMP) and tissue inhibitors of metalloproteinases (TIMP) in humans. Nine volunteers [5 men and 4 women, mean age 23 (SD 4) yr] each performed a bout of 100 maximum voluntary eccentric contractions of the knee extensors. Muscle biopsies were taken before exercise and on days 4 and 22 afterward. Image analysis of stained tissue sections was used to quantify endomysial collagen staining intensity. Maximum voluntary contractile isometric force was recorded preexercise and on days 1, 2, 3, 4, 8, 11, and 14 postexercise. Venipuncture blood samples were also drawn on these days for measurement of serum creatine kinase activity and concentrations of MMP-9, TIMP-1, TIMP-2, and the MMP-2/TIMP-2 complex. Maximum voluntary contractile force declined by 39 ± 23% (mean ± SD) on day 2 postexercise and recovered thereafter. Serum creatine kinase activity peaked on day 4 postexercise ( P < 0.01). Collagen type IV staining intensity increased significantly on day 22 postexercise to 126 ± 29% (mean ± SD) of preexercise values ( P < 0.05). Serum MMP-9 levels increased on day 8 postexercise ( P < 0.01), and serum TIMP-1 was also significantly elevated on days 1, 2, 3, 4, and 14 postexercise ( P < 0.05). These results suggest that a single bout of eccentric muscle contractions results in remodeling of endomysial type IV collagen, possibly via the MMP pathway.

Inhibition of creatine kinase activity by lysine in rat cerebral cortex

2009 ◽  
Vol 24 (2) ◽  
pp. 349-360 ◽  
Author(s):  
Anelise Miotti Tonin ◽  
Gustavo Costa Ferreira ◽  
Patrícia Fernanda Schuck ◽  
Carolina Maso Viegas ◽  
Ângela Zanatta ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Cerebral Cortex ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
Rat Cerebral Cortex

A "Reagentless" Fluorometric Method for Creatine Kinase Activity in Serum

1973 ◽  
Vol 19 (9) ◽  
pp. 1045-1048 ◽  
Author(s):  
Herbert K Y Lau ◽  
George G Guilbault
Keyword(s):  
Creatine Kinase ◽  
Silicone Rubber ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
Serum Creatine Kinase ◽  
Fluorometric Method ◽  
Nadh Fluorescence

Abstract A "reagentless" fluorometric method is described for the analysis of serum creatine kinase (CK) activity. The method is based on the use of silicone rubber pads, upon which are placed all the reagents for assay of CK. The rate of formation of NADH fluorescence at 460 nm is measured and equated to CK activity. The method is simple, rapid, inexpensive, and as little as 3 µl of serum is needed.

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